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{"sheet":"scRNA-seq protocols","index":0,"n_rows":52,"n_cols":10,"rows_kept":52,"cut":false,"hidden":false,"merged":[]}
["Name","Reference","Format","Amplification","UMI","Cellbarcodes","Coverage","Note","Multi-omics","Commercial system"]
["Tang protocol","Tang et al. 2009","microwellplates/tubes","PCR","no","no","3' biased"]
["STRT-seq","Islam et al. 2011","microwellplates/tubes","PCR","no","6 bp","5' ends"]
["Smart-seq","Ramsköld et al. 2012","microwellplates/tubes","PCR","no","no","full-length"]
["CEL-seq","Hashimshony et al. 2012","microwellplates/tubes","IVT","no","8 bp","3' ends"]
["Digital RNA sequencing","Shiroguchi et al., 2012","microwellplates/tubes","?","20 bp","no","?","designed UMI sequences"]
["Quartz-Seq","Sasagawa et al., 2013","microwellplates/tubes","PCR","no","no","3' ends"]
["DP-seq","Barghava et al., 2013","microwellplates/tubes","PCR","no","no","partial"]
["Smart-seq2","Picelli et al., 2013","microwellplates/tubes","PCR","no","no","full-length"]
["Smart-seq/C1","Pollen et al., 2014","microfluidic device","PCR","no","no","full-length",null,null,"Fluidigm C1"]
["TIVA","Lovatt et al., 2014","microwellplates/tubes","IVT","no","no","?",null,"spatial information"]
["STRT-UMI","Islam et al. 2014","microfluidic device","PCR","5 bp","6 bp","5' ends"]
["SCRB-seq","Soumillon et al., 2014","microwellplates/tubes","PCR","10 bp","6 bp","3' ends",null,null,"Wafergen\tICELL8"]
["CEL-seq UMI","Grün et al., 2014","microwellplates/tubes","IVT","4 bp","8 bp","3' ends"]
["MARS-seq","Jaitin et al., 2014","microwellplates/robotic platform","IVT","6 bp","8 bp","3' ends"]
["Drop-seq","Macosko et al., 2015","microdroplets","PCR","8 bp","12 bp","3' ends",null,null,"Dolomite Single Cell RNA-Seq System"]
["InDrops","Klein et al., 2015","microdroplets","IVT","6 bp","~19 bp","3' ends",null,null,"1CellBio InDrops"]
["Cyto-seq","Fan et al., 2015","nanowells","PCR","8 bp","24 bp","3' ends"]
["SC3-seq","Nakamura et al., 2015","microwellplates/tubes","PCR","no","6 bp","3' ends"]
["BAT-seq","Velten et al., 2015","microwellplates/tubes","IVT","6 bp","6 bp","3' ends"]
["SUPeR-seq","Fan et al., 2015","microwellplates/tubes","PCR","no","no","full-length","total RNA analysis"]
["DR-seq","Dey et al., 2015","microwellplates/tubes","IVT","4 bp","8 bp","3' ends",null,"WGS from same cell"]
["G&T-seq","Macaulay et al., 2015","microwellplates/tubes","PCR","no","no","full-length",null,"WGS from same cell"]
["Hi-SCL","Rotem et al., 2015","microdroplets","PCR","no","10 bp","3' ends"]
["SORT-seq","Muraro et al., 2016","microwellplates","IVT","4 bp","8 bp","3' ends","miniaturized reactions"]
["CEL-seq2","Hashimshony et al. 2016","microwellplates or microfluidics","IVT","6 bp","8 bp","3' ends"]
["Patch-seq","Cadwell et al., 2016","microwellplates/tubes","PCR","no","no","full-length",null,"elecrophysiology measurement"]
["Patch-seq","Fuzik et al., 2016","microwellplates/tubes","PCR","5 bp","6 bp","5' ends",null,"elecrophysiology measurement"]
["Smart-seq2-nuclei","Krishnaswami et al., 2016","microwellplates","PCR","no","no","full-length","single nuclei"]
["FRISCR","Thomsen et al., 2016","microwellplates/tubes","PCR","no","no","full-length","fixed cells"]
["Div-Seq","Habib et al., 2016","microwellplates","PCR","no","no","full-length","single, dividing neurons"]
["LCM-seq","Nichterwitz et al., 2016","microwellplates/tubes","PCR","no","no","full-length","LCM cell isolation"]
["scTrio-seq","Hou et al., 2016","microwellplates/tubes","PCR","no","no","full-length",null,"methylation + genome from same cell"]
["scM&T-seq","Angermueller et al., 2016","microwellplates/tubes","PCR","no","no","full-length",null,"methylation data from same cell"]
["MASC-seq","Vickovic et al., 2016","array slides","IVT","9 bp","18 bp","3' ends",null,"allows imaging"]
["Spatial transcriptomics","Stahl et al, 2016","array slides","IVT","9 bp","18 bp","3' ends",null,"Spatial information by spatial barcoding on an array (200µm resolution)"]
["SureCell","Illumina 2016","microdroplets","PCR","yes","yes","3' ends","direct tagmentation",null,"Illumina/BioRad ddSEQ"]
["MATQ-seq","Sheng et al., 2017","microwellplates","PCR","6 bp","no","full-length","MALBAC-RT"]
["10x Genomics","Zheng et al., 2017","microdroplets","PCR","10 bp","14 bp","3' ends",null,null,"10x Genomics Chromium"]
["Seq-Well","Gierahn et al., 2017","nanowells","PCR","8 bp","12 bp","3' ends"]
["SPLiT-seq","Rosenberg et al., 2017","Split/pool in-situ barcoding","PCR","10 bp","24 bp","3' ends"]
["sci-RNA-seq","Cao et al., 2017","Split/pool in-situ barcoding","PCR","10 bp","10 bp","3' ends"]
["DroNc-seq","Habib et al., 2017","microdroplets","PCR","8 bp","12 bp","3' ends","nuclei sequencing"]
["sNucDrop-Seq","Hu et al., 2017","microdroplets","PCR","8 bp","12 bp","3' ends","nuclei sequencing"]
["STRT-2i","Hochgerner et al., 2017","nanowells","PCR","6 bp","13 bp","5' ends"]
["snoRNA-seq","Faridani et al., 2017","microwellplates/tubes","PCR","8 bp","no","full-length","small RNA transcriptome"]
["Quartz-Seq2","Sasagawa et al., 2017","microwellplates","PCR","8 bp","15 bp","3' ends"]
["GEO-seq","Chen et al., 2017","microwellplates/tubes","PCR","no","no","full-length","LCM cell isolation"]
["REAP-seq","Peterson et al., 2017","microdroplets","PCR","10 bp","14 bp","3' ends",null,"protein abundance from same cell (barcoded antibodies)"]
["CITE-seq","Stoeckius et al., 2017","microdroplets","PCR","8 bp","12 bp","3' ends",null,"protein abundance from same cell (barcoded antibodies)"]
["mcSCRB-seq","Bagnoli et al., 2017","microwellplates","PCR","10 bp","6 bp","3' ends"]
["Niche-seq","Medaglia et al., 2017","microwellplates","IVT","6 bp","8 bp","3' ends","scRNA-seq is MARS-seq","Spatial information by photoactivatable tag"]