This PDF file includes:

• Fig. S1. Quantification of all proteins expressed in FD-CF.
• Fig. S2. Fluorescent proteins expressed in the PURE and crude extract systems.
• Fig. S3. Quantitative analysis of fluorescent proteins.
• Fig. S4. Representative scanning electron microscopy images of hydrogel ultrastructures generated with FD-CF enzymes.
• Fig. S5. Schematic of RPA reaction.
• Fig. S6. Detailed steps for isolating genomic DNA from fruits for environmental sensing activity.
• Table S1. Library of proteins and toehold switches that enable visual, olfactory, and tactile outputs for educational engagement.
• Table S2. FD-CF reactions allow for inexpensive classroom synthetic biology education kits.

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