This PDF file includes:

• Fig. S1. Overview of single-particle cryo-EM for hTRPV6-CaM.
• Fig. S2. Comparison of cryo-EM densities for hTRPV6, hTRPV6-CaM, and rTRPV6-CaM.
• Fig. S3. Cryo-EM density for hTRPV6-CaM.
• Fig. S4. Structures of CaM bound to ion channel fragments.
• Fig. S5. Overview of single-particle cryo-EM for rTRPV6-CaM.
• Fig. S6. Cryo-EM density for rTRPV6-CaM.
• Fig. S7. Comparison of hTRPV6-CaM and rTRPV6-CaM.
• Fig. S8. Sequence alignment of TRPV subunits.
• Fig. S9. Superposition of the C termini in different TRPV6 subunits.
• Fig. S10. hTRPV6 selectivity filter from different gating states.
• Table S1. Cryo-EM data collection, refinement, and validation statistics.
• Reference (42)

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