{"text_format":"2","workbook":"xlsx","n_sheets":3,"sheets":["plasmid constructs","antibodies","cell lines"],"not_rendered":{"charts":0,"images":0,"macros":0,"external_links":0,"defined_names":0,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"run_cost":"one","rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900"}

{"sheet":"plasmid constructs","index":0,"n_rows":21,"n_cols":3,"rows_kept":21,"cut":false,"hidden":false,"merged":[]}
["Expressed protein","Promotor","Description"]
["UBTOR-FLAG-HA","SV40"," C-terminal of UBTOR tagged with FLAG and HA"]
["UBTOR-EGFP","CMV"," C-terminal of UBTOR tagged with EGFP"]
["UBTOR1-851-EGFP","CMV"," C-terminal of UBTOR1-851 tagged with EGFP"]
["FLAG-UBTOR","CMV"," N-terminal of UBTOR tagged with FLAG"]
["FLAG-UBTOR1-895","CMV"," N-terminal of UBTOR1-895 tagged with FLAG"]
["FLAG-UBTOR1-851","CMV"," N-terminal of UBTOR1-851 tagged with FLAG"]
["FLAG-UBTOR1-467","CMV"," N-terminal of UBTOR1-467 tagged with FLAG"]
["UBTOR∆1-81-EGFP","CMV"," C-terminal of UBTOR∆1-81-EGFP tagged with EGFP"]
["UBTOR∆1-160-EGFP","CMV"," C-terminal of UBTOR∆1-160-EGFP tagged with EGFP"]
["FLAG-UBTOR∆468-773","CMV"," N-terminal of UBTOR∆468-773: tagged with FLAG"]
["GST-UBTOR1-467","tac"," N-terminal of UBTOR1-467 tagged with GST"]
["FLAG-DEPTOR","CMV"," N-terminal of DEPTOR tagged with FLAG"]
["HA-DEPTOR","CMV"," N-terminal of DEPTOR tagged with HA"]
["3HA-DEPTOR","CMV"," N-terminal of DEPTOR tagged with 3 X HA"]
["His/HA-DEPTOR","T7lac"," N-terminal of DEPTOR tagged with 6 X histamine and 2 X HA"]
["His/HA-2DEP","T7lac"," N-terminal of DEPTOR 2DEP domain tagged with 6 X histamine and 2 X HA"]
["His/HA-PDZ","T7lac"," N-terminal of DEPTOR PDZ domain tagged with 6 X histamine and 2 X HA"]
["FLAG-MLST8","CMV"," N-terminal of MLST8 tagged with FLAG"]
["HA-Ub","CMV"," N-terminal of ubiquitin tagged with HA"]
["EGFP-HRAS-G12V","CMV"," N-terminal of HRAS-G12V mutation tagged with EGFP"]

{"sheet":"antibodies","index":1,"n_rows":40,"n_cols":4,"rows_kept":40,"cut":false,"hidden":false,"merged":[]}
["antigen","source","vendor","catalog number"]
["FLAG","Mouse monoclonal","Abmart","M20008"]
["GFP","Mouse monoclonal","Roche",1181446]
["MYC","Rabbit polyclonal","Proteintech",16286]
["GST","Rabbit monoclonal","Cell Signaling Technology",5475]
["PDIA4","Rabbit polyclonal","Proteintech",641581]
["CANX","Rabbit polyclonal","Proteintech",10427]
["UBTOR","Rabbit polyclonal","Sigma-aldrich","HPA011545"]
["acetylated tubulin","Mouse monoclonal","Sigma-aldrich","T7451"]
["mTOR ","Rabbit polyclonal","Proteintech",20657]
["mTOR ","Rabbit monoclonal","Cell Signaling Technology",2983]
["Raptor","Rabbit monoclonal","Cell Signaling Technology",2280]
["Rictor","Rabbit monoclonal","Cell Signaling Technology",2114]
["MLST8","Rabbit monoclonal","Cell Signaling Technology",3274]
["DEPTOR","Rabbit polyclonal","Millipore","ABS222"]
["S6","Rabbit polyclonal","Abcam","ab40820"]
["p-S6(Ser235/236)","Rabbit monoclonal","Cell Signaling Technology",4858]
["S6K","Rabbit monoclonal","Cell Signaling Technology",2708]
["p-S6K","Rabbit monoclonal","Cell Signaling Technology",9234]
["AKT","Rabbit polyclonal","Cell Signaling Technology",9272]
["p-AKT(s473)","Rabbit monoclonal","Cell Signaling Technology",4060]
["ERK(1/2)","Mouse monoclonal","Cell Signaling Technology",9107]
["p-ERK(1/2)","Rabbit polyclonal","Cell Signaling Technology",9101]
["HA","Rabbit polyclonal","Proteintech",51064]
["ubiquitin","Mouse monoclonal","Millipore","ST1200"]
["Ki67","Mouse monoclonal","Cell Signaling Technology",9449]
["β-actin","Mouse monoclonal","Abmart","ab167453"]
["GAPDH","Rabbit polyclonal","Proteintech","M20010"]
[null,null,null,10494]
[]
["Anti-Digoxigenin-AP, Fab fragments","Sheep","Roche",11093274910]
["anti-mouse IgG (H+L), Secondary Antibody, Alexa Fluor 546conjugate","Goat","ThermoFisher","A-11003"]
["anti-Rabbit IgG (H+L), Secondary Antibody, Alexa Fluor 546 conjugate","Goat","ThermoFisher","A-11035"]
["anti-mouse IgG (H+L), Secondary Antibody, Alexa Fluor 488 conjugate","Goat","ThermoFisher","A-11001"]
["anti-Rabbit IgG (H+L), Secondary Antibody, Alexa Fluor 488 conjugate","Goat","ThermoFisher","A-11034"]
["anti-mouse IgG (H+L), Secondary Antibody, Alexa Fluor 647 conjugate","Goat","ThermoFisher","A-21235"]
["anti-Rabbit IgG (H+L), Secondary Antibody, Alexa Fluor 647 conjugate","Chicken","ThermoFisher","A-21443"]
["Anti-rabbit IgG, HRP-linked antibody","Goat","Cell Signaling Technology",7074]
["Anti-mouse IgG, HRP-linked antibody","Horse ","Cell Signaling Technology",7076]
["Clean-Blot Detection Reagent (HRP)",null,"Thermo Fisher Scientific",21232]

{"sheet":"cell lines","index":2,"n_rows":7,"n_cols":3,"rows_kept":7,"cut":false,"hidden":false,"merged":[]}
["Name","Source","Note"]
["ld-PC12","Chinese Academia of Sciences Cell Bank","a subline of PC-12, exhibits adherant phenotypes"]
["PC12","Chinese Academia of Sciences Cell Bank"]
["HEK293T","Chinese Academia of Sciences Cell Bank"]
["T24","Chinese Academia of Sciences Cell Bank"]
["HeLa","Chinese Academia of Sciences Cell Bank"]
["hT-RPE","gift from Dr. J Zhu"]