This PDF file includes:

• Fig. S1. Principle of FD-based AFM to probe HV binding to living animal cells.
• Fig. S2. Characterization of MuHV-4 viral particles and validation of tip and surface functionalization.
• Fig. S3. Attachment and infection of GAG⁺ and GAG⁻ CHO cells.
• Fig. S4. Exploring a wide range of LRs with FD-based AFM.
• Fig. S5. Control experiments showing the specificity of MuHV-4 virus binding toward immobilized heparin.
• Fig. S6. Binding force distribution and multipeak Gaussian fit of different LR sets.
• Fig. S7. Multipeak Gaussian fit of average rupture forces of gp150⁻ MuHV-4 binding extracted for discrete LRs.
• Fig. S8. Principle of combined optical and FD-based AFM imaging of animal cells to extract topography images and quantitative multiparametric maps.
• Fig. S9. Consecutive mapping of WT virus binding to CHO cells shows similar results.
• Fig. S10. Investigation of virus binding to cells using flow cytometry.

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