This PDF file includes:

• Fig. S1. Correlations between methylation and gene expression levels in subcutaneous adipose tissue and skeletal muscle at baseline and in response to acute sleep loss in humans.
• Fig. S2. Insulin sensitivity is adversely affected, and cortisol levels are significantly elevated following acute sleep loss in healthy young men without changes to protein levels of mitochondrial complexes.
• Fig. S3. Gene expression and protein levels correlate at baseline but not in response to sleep loss in subcutaneous adipose tissue and skeletal muscle in humans.
• Fig. S4. Loading controls for Western blots used throughout the manuscript.
• Table S1. Data for the 15 participants that were included in the study.
• Table S2. DMRs and enriched biological pathways based on methylation changes in subcutaneous adipose tissue in response to acute sleep loss.
• Table S3. Differentially expressed genes in skeletal muscle and subcutaneous adipose tissue in response to acute sleep loss.
• Table S4. Altered pathways and transcription factors based on RNA-seq data from skeletal muscle and subcutaneous adipose tissue in response to acute sleep loss compared with normal sleep.
• Table S5. Enriched pathways based on proteomic analyses of skeletal muscle tissue in response to acute sleep loss compared with normal sleep.
• Table S6. Changes in serum, skeletal muscle, and subcutaneous adipose tissue metabolites in response to acute sleep loss.
• Supplementary Methods
• References (62–82)

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