This PDF file includes:

• Fig. S1. Molecular screening of transgenic plants.
• Fig. S2. Efficacy of the genome editing constructs in vitro and in vivo.
• Fig. S3. Screening of predicted off-target cleavage sites for GBSSsgRNA4 and PTSTsgRNA2.
• Fig. S4. Morphology of glasshouse-cultivated gbss and ptst lines.
• Fig. S5. CLDs in storage root starch of gbss and ptst lines.
• Fig. S6. Flow cytometry analysis of starch granule size in gbss and ptst lines.
• Fig. S7. Scanning electron microscopy of purified gbss and ptst starch granules.
• Fig. S8. Physicochemical properties of gbss and ptst starch.

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