This PDF file includes:

• Fig. S1. Expression and purification of LmrA proteins.
• Fig. S2. Peptide coverage maps from the Mascot LC-MS/MS database search results.
• Fig. S3. Ion binding sites in LmrA.
• Fig. S4. Binding sites for Na⁺ and Cl⁻ in example proteins.
• Fig. S5. Expression and purification of LmrA-N137A mutant protein.
• Fig. S6. Conservation of residue N137 in ABC multidrug transporters.
• Table S1. Mascot search results for mass spectrometry data for purified LmrA-WT.
• Table S2. Mascot search results for mass spectrometry data for purified LmrA-ΔK388.
• Table S3. Speciation of HEPES at pH 6.5 as a function of the HEPES concentration.
• Data analysis S1. Determination of E_rev values and ion stoichiometry.
• Data analysis S2. Comparisons of ion transport models.

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