{"text_format":"2","workbook":"xlsx","n_sheets":6,"sheets":["RIP","ITGB1A and 1D","RT-PCR","Silencing sequences","vector construction","RNA-seq validation"],"not_rendered":{"charts":0,"images":0,"macros":0,"external_links":0,"defined_names":0,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"run_cost":"one","rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900"}

{"sheet":"RIP","index":0,"n_rows":9,"n_cols":3,"rows_kept":9,"cut":false,"hidden":false,"merged":[[0,0,0,2]]}
["RIP assays RT-PCR primers"]
[null,"F-sequence (5’®3’)","R-sequence (5’®3’)"]
["U1(rat)","TACTTACCTGGCAGGGGAGATAC","GAACGCAGTCCCCCACTAC"]
["U2(rat)","CTCGGCCTTTTGGCTAAGAT","ACTGCAATACCAGGTCGATG"]
["U4(rat)","TGCAGTGGCAGTATCGTAGC","AAAATTGCCAATGCCGACTA"]
["U5(rat)","CTCTGGTTTCTCTTCAGATCGT","GCTCAAGACAAGGCCTCAAA"]
["U6(rat)","TGCTTCGGCAGCACATATAC","AAAATATGGAACGCTTCACGA"]
["ITGB1-D(rat)","CGTGGTTGCCGGAATTGTT","ACCAGCTTTACGTCCATAGTTTG"]
["ITGB1-Pre-mRNA(human)","TGGCCTTGCATTACTGCTGA","AGCCACTTTGCTTTTTGGATG"]

{"sheet":"ITGB1A and 1D","index":1,"n_rows":8,"n_cols":3,"rows_kept":8,"cut":false,"hidden":false,"merged":[[0,0,0,2],[5,0,5,2]]}
["Primer sequences for ITGB1A and ITGB1D producted from the minigene"]
[null,"F-sequence (5’®3’)","R-sequence (5’®3’)"]
["mini-1A+1D (human)","TCATGACAGAAGGGAGTTTGCT","CCACAGTTGTTACGGCACTCTTA"]
[]
[]
["Primer sequences for endogenous ITGB1A and ITGB1D"]
[null,"F-sequence (5’®3’)","R-sequence (5’®3’)"]
["ITGB1-A+D (rat/mouse)","TTGTGGAGACTCCAGACTGTCCTACT","TCATTTTCCCTCATACTTCGGATT"]

{"sheet":"RT-PCR","index":2,"n_rows":18,"n_cols":3,"rows_kept":18,"cut":false,"hidden":false,"merged":[[0,0,0,2]]}
["RT-PCR primers"]
[null,"F-sequence (5’®3’)","R-sequence (5’®3’)"]
["PTBP1(rat)","GACGTGCTGCACCAGATCTTC","GCATACGGAGAGGCTGACATTA"]
["LRP6(rat/mouse/human)","GGCGGTGTGATGGGTTTACT","CTGTAGGATGGTGGTGGGTTC"]
["18s rRNA（rat）","GCAATTATTCCCCATGAACG","GGCCTCACTAAACCATCCAA"]
["integrin β1（rat）","AATGTTTCAGTGCAGAGCC","TTGGGATGATGTCGGGAC"]
["β-catenin(human)","CATCTACACAGTTTGATGCTGCT","GCAGTTTTGTCAGTTCAGGGA"]
["β-catenin(rat)","ATGCCGTTCGCCTTCATTA","GGACCCCTGCAGCTACTCTTT"]
["β-catenin(mouse)","ATGGAGCCGGACAGAAAAGC","CTTGCCACTCAGGGAAGGA"]
["GAPDH(human)","GAAGGTGAAGGTCGGAGTC","GAAGATGGTGATGGGATTTC"]
["GAPDH(rat)","AGTTCAACGGCACAGTCAAG","TACTCAGCACCAGCATCACC"]
["GAPDH(mouse)","AGGTCGGTGTGAACGGATTTG","TGTAGACCATGTAGTTGAGGTCA"]
["β-actin(rat)","ATGGATGACGATATCGCTG","ATGAGGTAGTCTGTCAGGT"]
["ITGB1D (rat)","CGTGGTTGCCGGAATTGTT","ACCAGCTTTACGTCCATAGTTTG"]
["Troponin T (mouse)","CCTGTGGTGCCTCCTTTGATT","TGCGGTCTTTTAGTGCAATGAG"]
["α-actin (mouse)","AGATTGTGCGCGACATCAAAG","GCAGACTCCATACCGATAAAGGA"]
["MLC3(mouse)","AGTGCTGACCAGATTGCCG","TCATGGGCAGAAACTGTTCAAA"]
["MyHC(mouse)","TGGAGGGTGAGGTAGAGAGTG","TTGGATAGATTTGTGTTGGATTG\n"]

{"sheet":"Silencing sequences","index":3,"n_rows":10,"n_cols":3,"rows_kept":10,"cut":false,"hidden":false,"merged":[[0,0,0,2]]}
["SiRNA Sequence"]
[null,"Sense 5'®3'","Antisense 5'®3'"]
["si-Lrp6 (rat)","GGCAGACACAGGAACAAAUdTdT","AUUUGUUCCUGUGUCUGCCdTdT"]
["si-Lrp6(human)","ACAUUGUUCUGCAGUUAGCdTdT","GCUAACUGCAGAACAAUGUdTdT"]
["si-β-catenin(human)","GCUGAAACAUGCAGUUGUAdTdT","UACAACUGCAUGUUUCAGCdTdT"]
["si-β-catenin(rat)","GAGACUGCCUUCAGAUCUUdTdT","AAGAUCUGAAGGCAGUCUCdTdT"]
["si-β-catenin(mouse)","GCACCAUGCAGAAUACAAAdTdT","UUUGUAUUCUGCAUGGUGCdTdT"]
["si-hnRNP-A1(rat)","GAAGAGUUGUGGAACCUAA dTdT","UUAGGUUCCACAACUCUUCdTdT"]
["si-Ptbp1(rat)","GCACAGUCCUGAAGAUCAUdTdT","AUGAUCUUCAGGACUGUGCdTdT"]
["Scramble siRNA","UUCUCCGAACGUGUCACGUdTdT","ACGUGACACGUUCGGAGAAdTdT"]

{"sheet":"vector construction","index":4,"n_rows":31,"n_cols":3,"rows_kept":31,"cut":false,"hidden":false,"merged":[[0,0,0,2]]}
["vector construction primers"]
["plasmids  name","primer  F-sequence (5’®3’)","primer  R-sequence (5’®3’)"]
["pcDNA3.1-flag-Lrp6-P1E1P2E2","CGGGATCCATGGGGGCCGTCCTGAGGAG","GCTCTAGAGACAATGCAGGTCTTCATGTCACTGATGA"]
["pcDNA3.1-flag-Lrp6-P3E3P4E4","CGGGATCCCCAGAGGCTTTCCTTTTGTTTT","GCTCTAGAACATGATAGCTCATCTTGAAGTAGAACC"]
["pcDNA3.1-flag-Lrp6-ICD","CGGGATCCGGAGAACCTCCAACATGTTCTCCT","GCTCTAGAGGAGGAGTCTGTACAGGGAGAGGG"]
["pcDNA3.1-flag-Lrp6","CGGGATCCATGGGGGCCGTCCTGAGGAG","GCTCTAGAGGAGGAGTCTGTACAGGGAGAGGG"]
["pCMV6-entry-U1 (rat)","GCCGCGATCGCGATACTTACCTGGCAGGGGAGATACC","CCGCTCGAGCAGGGGAGAGCGCGAACGCAGTC"]
["pCMV6-entry-U2 (rat)","GCCGCGATCGCGATCGCTTCTCGGCCTTTTGGCTAAG","CCGCTCGAGTGGTGCACCGTTCCTGGAGGTACT"]
["pCMV6-entry-U4 (rat)","GCCGCGATCGCGAACTTTGCAGTGGCAGTATCGT","CCGCTCGAGCAGTCTCCGTAGAGACAAAAATTGC"]
["pCMV6-entry-U5 (rat)","GCCGCGATCGCATACTCTGGTTTCTCTTCAGATCGTA","CCGCTCGAGAGCCTTGCTCAAGACAAGGCCTCA"]
["pCMV6-entry-U6 (rat)","GCCGCGATCGCGGTGCCTGCTTCGGCAGCACATATA","CCGCTCGAGAAAATATGGAACGCTTCACGAAT"]
["human pGL3-ITGB1 promoter","CCGGAGCTCTGAACAATGGTAAAACCGATACCTTTAAAATTCAA","CCGCTCGAGCTGCGCTTGCCCAGCCCGGCGGGAGACCGCA"]
["pCMV6-entry-NLS-GFP-ICDWT/LCDMut","GAGCTGTACAAGGGAGAACCTCCAACATGTTCTCCTCAGCAGTTT","GCGGCCGCTTTAGGAGGAGTCTGTACAGGGAGAGGGTGGCGGTGGG"]
["pCMV6-entry-mini-fragment-1","GCCGCGATCGCCATTATTACCTTCTTATTTTCCAGAGT","TGTGAGTTTTTGTTTTAAAGAAAGAAGCAG"]
["pCMV6-entry-mini-fragment-2","CTTTAAAACA AAAACTCACA GAACTATGTA","TCAAAACGGGTTATGAGGATTAGAGCTGAC"]
["pCMV6-entry-mini-fragment-3","ATCCTCATAACCCGTTTTGATTAGCAGGCG","CCGCTCGAGTCAATCGCTATAGAAATATCACTTAATCCA"]
["pCMV6-entry-mini","GCCGCGATCGCCATTATTACCTTCTTATTTTCCAGAGT","CCGCTCGAGTCAATCGCTATAGAAATATCACTTAATCCA"]
["pCMV6-entry-M1","ATGTTTCCTTTCTACGGACGTAAAGCT","TAGAAAGGAAACATGAAATTATTAATAGGACTCTTGT"]
["pCMV6-entry-M2","AATTTCAACAATCCAAACTACGGAC","GGATTGTTGAAATTATTAATAGGACTCTTGTA"]
["pCMV6-entry-M3","ATGGAAACTACGGACGTAAAGCTGGTC","GTCCGTAGTTTCCATTCTTGAAATTATTAATAGGA"]
["pCMV6-entry-M4","AACCAAACTACGGACGTAAAGCTGGTCT","GTCCGTAGTTTGGTTTCTTGAAATTATTAATAGGA"]
["pCMV6-entry-M5","AAGTAGGGAGGTAAAGCTGGTCTCTAAATTG","TTACCTCCCTACTTTGGATTCTTGAAATTAC"]
["pCMV6-entry-M6","AACTTCGGTCGTAAAGCTGGTC","TTACGACCGAAGTTTGGATTCTT"]
["pCMV6-entry-M7","AACAACGGACGAAAAGCTGGTC","TTTCGTCCGTTGTTTGGATTCTT"]
["pCMV6-entry-M8","AACTACCCACCTAAAGCTGGTCTCTAAATTG","TTAGGTGGGTAGTTTGGATTCTTGAAATTATT"]
["pCMV6-entry-Mut-reporter-1","AAAATCGAGTCCTATTAATAATTTCAAGA","GGACTCGATTTTCTTGCTAAAGTAAA"]
["pCMV6-entry-Mut-reporter-2","TTACAAAATAATTTCAAGAATCCAAA","ATTATTTTGTAAATCGGATTTTCTTG"]
["pCMV6-entry-Mut-reporter-3","CCTATTAGAATCCAAA CTACGGACG","GATTCTAATAGGACTCTTGTAAATCGGA"]
["pCMV6-entry-Mut-reporter-4","ATTTCACTACGGACGTAAAGCTG","CCGTAGTGAAATTATTAATAGGACTCTTG"]
["pCMV6-entry-Mut-reporter-5","TCCAAAAAAGCTGGTCTCTAAATT","AGCTTTTTTGGATTCTTGAAATTATTAAT"]
["pCMV6-entry-Mut-reporter-6","GGACGTTCTAAATTGCCGTTCGTAT","TTTAGAACGTCCGTAGTTTGGATTCTTG"]

{"sheet":"RNA-seq validation","index":5,"n_rows":8,"n_cols":3,"rows_kept":8,"cut":false,"hidden":false,"merged":[[0,0,0,2]]}
["PCR primers"]
[null,"F-sequence (5’®3’)","R-sequence (5’®3’)"]
["apla2(Rat)","ATGGACCAGTTTACCTCCTCCA","CCTTTTCTTCTGCACCAATCAG"]
["PLOD2(Rat)","GCGTGATAAGTTGGATCCCGAC","CTGCCAGAGGTCATTGTTGAGATG"]
["FN1-1(Rat)","GAAGTGGTTCATGCCGATCAGAG","CTCTGCCACATCCTCCTCGTTC"]
["MACF1(Rat)","GTAAAGGAGTGCTGGT","ACCACTGTAATACAGAGCTCGCC"]
["FN1-2(Rat)","CCGAGTACACCATCTATGTCATCGC","AGTGCCAACAGGTTGGCATGA"]
["Mbnl1(Rat)","CAGCAGAAGAACATGGCCATGC","GACCAACATTGGTGCAGTCGG"]