FungiDiaporthalesDiaporthaceaeYangQinFanXin-LeiGuarnacciaVladimiroTianCheng-MingHigh diversity of Diaporthe species associated with dieback diseases in China, with twelve new species describedMycoKeys17920182018399714910.3897/mycokeys.39.26914 Diaporthe unshiuensis F. Huang, K.D. Hyde & H.Y. Li, 2015Figure 17Description.

On PNA: Conidiomata pycnidial, globose or rostrated, black, erumpent in tissue, erumpent at maturity, 260–500 μm diam, often with translucent conidial drops exuding from the ostioles. Conidiophores 18–28.5 × 1.4–2.1 μm, cylindrical, hyaline, branched, septate, straight or curved, tapering towards the apex. Alpha conidia abundant in culture, hyaline, aseptate, ellipsoidal to fusiform, biguttulate, sometimes with one end obtuse and the other acute, 6.5–8.5 × 2.1–2.5 μm (av. = 7.8 × 2.3 μm, n = 30). Beta conidia not observed.

Diaportheunshiuensis (CFCC 52594) A Culture on PNA B Conidiomata C Alpha conidia D Conidiophores. Scale bars: 500 μm (B), 10 μm (C–D).

Culture characters.

Cultures incubated on PNA at 25 °C in darkness. Colony entirely white at surface, reverse with pale brown pigmentation, white, fluffy aerial mycelium.

Specimens examined.

CHINA. Jiangsu Province: Nanjing city, on non-symptomatic twigs of Caryaillinoensis, 10 Nov. 2015, Q. Yang, living culture CFCC 52594 and CFCC 52595 (BJFC-S1476).

Notes.

Diaportheunshiuensis was originally described from twigs of non-symptomatic Fortunellamargarita in Zhejiang Province, China (Huang et al. 2015). In the present study, two isolates from twigs of asymptomatic Caryaillinoensis were congruent with D.unshiuensis based on morphology and DNA sequences data (Fig. 1). We therefore describe D.unshiuensis as a known species for this clade.

Phylogram of Diaporthe from a maximum likelihood analysis based on combined ITS, cal, his3, tef1 and tub2. Values above the branches indicate maximum likelihood bootstrap (left, ML BP ≥ 50%) and bayesian probabilities (right, BI PP ≥ 0.70). The tree is rooted with Diaporthellacorylina. Strains in the current study are in blue.

HuangFUdayangaDWangXHouXMeiXFuYHydeKDLiHY (2015) Endophytic Diaporthe associated with Citrus: A phylogenetic reassessment with seven new species from China.Fungal Biology119: 331–347. https://doi.org/10.1016/j.funbio.2015.02.006