Diagnosis.

Taxon classification

Fungi

Diaporthales

Diaporthaceae

Yang

Qin

Fan

Xin-Lei

Guarnaccia

Vladimiro

Tian

Cheng-Ming

High diversity of

Diaporthe

species associated with dieback diseases in China, with twelve new species described

MycoKeys179201820183997149

10.3897/mycokeys.39.26914

Diaporthe

cinnamomi

MB824709

C.M. Tian & Q. Yang

sp. nov.

Figure 10

Diagnosis.

Diaporthecinnamomi differs from its closest phylogenetic species D.discoidispora in ITS, his3 and tef1 loci based on the alignments deposited in TreeBASE.

Holotype.

CHINA. Zhejiang Province: Linan city, on symptomatic twigs of Cinnamomum sp., 22 Apr. 2017, Q. Yang (holotype: BJFC-S1482; ex-type culture: CFCC 52569).

Etymology.

Named after the host genus on which it was collected, Cinnamomum.

Description.

On PDA: Conidiomata pycnidial, globose, solitary or aggregated, deeply embedded in the substrate, erumpent, dark brown to black, 170–235 μm diam., whitish translucent to cream conidial drops exuding from the ostioles. Conidiophores 11–25 × 1.5–2 μm, cylindrical, hyaline, branched, straight or curved, tapering towards the apex. Alpha conidia hyaline, aseptate, ellipsoidal to oval, biguttulate, rounded at both ends, 5–7 × 2.5–3 μm (av. = 6 × 2.9 μm, n = 30). Beta conidia not observed.

Figure 10.

Diaporthecinnamomi (CFCC 52569) A Culture on PDAB Conidiomata C Alpha conidia D Conidiophores. Scale bars: 200 μm (B), 10 μm (C–D).

Culture characters.

Cultures incubated on PDA at 25 °C in darkness showed colony originally flat with white felty mycelium, developing petaloid mycelium after 7–10 d and turning yellowish at the centre and brownish at the marginal area after 15 d. Conidiomata erumpent at maturity.

Additional material examined.

CHINA. Zhejiang Province: Linan city, on symptomatic twigs of Cinnamomum sp., 22 Apr. 2017, Q. Yang, living culture CFCC 52570 (BJFC-S1483).

Notes.

Diaporthecinnamomi comprises strains CFCC 52569 and CFCC 52570 closely related to D.discoidispora in the combined phylogenetic tree (Fig. 1). Diaporthecinnamomi can be distinguished based on ITS, his3 and tef1 loci from D.discoidispora (4/460 in ITS, 17/448 in his3 and 38/339 in tef1).

Figure 1.

Phylogram of Diaporthe from a maximum likelihood analysis based on combined ITS, cal, his3, tef1 and tub2. Values above the branches indicate maximum likelihood bootstrap (left, ML BP ≥ 50%) and bayesian probabilities (right, BI PP ≥ 0.70). The tree is rooted with Diaporthellacorylina. Strains in the current study are in blue.