The PDF file includes:

• Fig. S1. Structural changes taking place and chemical shift restraints.
• Fig. S2. Strips from ¹H-¹H-NOESY-¹H-¹⁵N-HSQC spectra showing resonances emanating from residues 1074 and 1075 of the respective CID domains.
• Fig. S3. Strips from ¹H-¹H-NOESY ¹H-¹⁵N-HSQC spectra showing resonances emanating from residues 1076 and 1077 of the CID domains.
• Fig. S4. ¹⁵N relaxation data for the bound CID and NCBD domains and amide secondary chemical shifts of the complexes.
• Fig. S5. Backbone subnanosecond motions and ΔC_p for the three CID/NCBD complexes.
• Fig. S6. Frustration in the CID/NCBD complexes without electrostatics.
• Fig. S7. ¹H-¹⁵N-HSQC correlation spectra for bound and free CID and NCBD domains and the number of restraints in the structure determination as a function of amino acid residue.
• Fig. S8. Restraints used for structure calculations and refinement.
• Fig. S9. Urea and temperature denaturation experiments and Coulomb surfaces of the ancestral and extant CID/NCBD complexes.
• Legend for table S1

Download PDF

Other Supplementary Material for this manuscript includes the following:

• Table S1 (Microsoft Excel format). ITC parameters and intermolecular NOEs.