This PDF file includes:

• Fig. S1. Schematics of NS platform fabrication.
• Fig. S2. Photograph of NS cell culture reservoirs.
• Fig. S3. GFP and mCherry expression intensity after ratiometric cotransfection of GFP and mCherry by NES and LFN.
• Fig. S4. SEM of patterned NS platform.
• Fig. S5. Schematic of patterned NS fabrication.
• Fig. S6. Dosage control intracellular delivery of Cy5-tagged DNA oligomers.
• Fig. S7. Dosage control intracellular delivery of mCherry-tagged STIM1 protein fragment.
• Fig. S8. mCherry-tagged STIM1 protein fragment interaction with cell membrane protein Orai1.
• Fig. S9. GFP-tagged SpyCas9 RNP genome editing.
• Table S1. Cell culture media receipts.

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