This PDF file includes:

• Table S1. Sequence details for the gene circuits.
• Fig. S1. Biofilm formation of the control E. coli/pYYDT and light-responsive E. coli/pYYDT-RB strains in biofilm flow cell.
• Fig. S2. Inactivation of exogenous 3OC6-HSL by E. coli/(pYYDT-RB + pAiiO) cells and the cell-free supernatant.
• Fig. S3. Inactivation of exogenous 3OC6-HSL by E. coli/(pYYDT-RB + pAiiO) cells incubated in the dark, under NIR light and blue light.
• Fig. S4. Coculture biofilm formation by the mCHERRY-tagged control E. coli (QQ negative) and YFP-tagged P. stewartii.
• Fig. S5. CLSM images of mCHERRY-tagged light-responsive QQ E. coli biofilm and ConA-stained polysaccharide in the flow cell.
• Fig. S6. CLSM images of dead P. stewartii (YFP tagged) on preformed light-responsive QQ E. coli biofilm.

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