Ring finger protein 31–mediated atypical ubiquitination stabilizes forkhead box P3 and thereby stimulates regulatory T-cell function

Supporting Information

• RNF31 interacts with FOXP3 and induces atypical multi-mono-ubiquitination to FOXP3 and promotes its protein stability - Supplemental FIGURE 1. LUBAC members interact with FOXP3; Supplemental FIGURE 2. The domain mapping between FOXP3 and RNF31; Supplemental FIGURE 3. RNF31 mediates atypical ubiquitin conjugation of FOXP3; Supplemental FIGURE 4. Characterization of lysine residues of FOXP3 targeted by RNF31; Supplemental FIGURE 5. Commassie blue staining of purified proteins as indicated; Supplemental FIGURE 6. (A, B, C, D) The statistical analysis related to Fig. 7C, D, G, I respectively