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[]
["Supplementary Table S1: Average Nucleotide Identity (ANI) (%) based on whole genome alignments of Acidithiobacillus spp.. "]
["ANI values are colored red \naccording to historical species \ncutoff value (≥96%).","Acidithiobacillus thiooxidans A02","Acidithiobacillus thiooxidans DXS-W","Acidithiobacillus albertensis DSM 14366 ","Acidithiobacillus thiooxidans ZBY","Acidithiobacillus thiooxidans strain JYC-17","Acidithiobacillus thiooxidans strain CLST","Acidithiobacillus thiooxidans Licanantay","Acidithiobacillus thiooxidans GD1-3","Acidithiobacillus thiooxidans BY-02","Acidithiobacillus thiooxidans DMC","Acidithiobacillus thiooxidans ATCC 19377","Acidithiobacillus thiooxidans A01","Acidithiobacillus caldus DX","Acidithiobacillus caldus S1","Acidithiobacillus caldus ATCC 51756","Acidithiobacillus caldus SM-1","Acidithiobacillus caldus ZJ","Acidithiobacillus caldus strain MTH-04","Acidithiobacillus caldus ZBY.fna","Acidithiobacillus ferrooxidans ATCC 23270","Acidithiobacillus ferrooxidans ATCC 53993","Acidithiobacillus sp. GGI-221","Acidithiobacillus ferrooxidans Hel18","Acidithiobacillus ferrooxidans CCM 4253","Acidithiobacillus ferrooxidans YQH-1","Acidithiobacillus ferrooxidans DLC-5","Acidithiobacillus ferrooxidans IO-2C","Acidithiobacillus ferridurans JCM 18981","Acidithiobacillus sp. UBA2486","Acidithiobacillus sp. NORP59","Acidithiobacillus ferrivorans YL15","Acidithiobacillus ferrivorans SS3","Acidithiobacillus ferrivorans PRJEB5721","Acidithiobacillus ferrivorans PQ33","Acidithiobacillus ferrivorans CF27","Acidithiobacillus ferrooxidans BY0502","Acidithiobacillus ferrivorans 21-59-9","Acidithiobacillus sp. SH"]
["Acidithiobacillus thiooxidans A02","*",98.14,96.75,99.88,99.93,97.06,96.57,98.14,99.93,99.95,96.41,99.8,68.97,69.01,68.77,69.5,68.75,69.75,68.84,74.24,74.24,70.62,74.67,74.67,74.64,75.62,74.29,74.34,68.26,63.45,69.62,70.16,70,69.82,70,70.66,70.25,79.03]
["Acidithiobacillus thiooxidans DXS-W",97.81,"*",96.32,97.81,97.8,97.05,96.24,99.93,97.81,97.81,96,97.7,69.14,68.84,68.91,70.42,69.14,70.34,69.23,74.02,74.02,70.65,74.47,74.49,74.49,74.91,73.91,74.28,68.2,62.96,69.7,69.9,69.81,70.04,69.81,70.06,70.39,78.72]
["Acidithiobacillus albertensis DSM 14366 ",97.22,97.31,"*",97.24,97.23,97.15,97.22,97.31,97.23,97.22,96.8,97.21,68.4,68.76,68.41,69.53,68.44,69.87,68.51,73.77,73.77,70.27,73.82,73.83,73.75,74.36,74.01,74,68.23,62.93,69.26,69.33,69.5,69.47,69.5,69.98,69.95,78.62]
["Acidithiobacillus thiooxidans ZBY",99.9,98.18,96.65,"*",99.94,97.11,96.69,98.18,99.94,99.96,96.38,99.87,68.8,68.89,68.6,69.63,68.75,69.87,68.86,70.37,70.37,70.27,70.36,70.36,70.29,70.78,70.11,70.23,70.64,63.13,69.95,70.44,70.39,70.33,70.39,71.13,70.44,79]
["Acidithiobacillus thiooxidans JYC-17",99.9,98.04,96.64,99.9,"*",97.07,96.64,98.03,99.93,99.93,96.4,99.83,69.1,68.89,68.71,69.29,69.08,68.86,69.13,74.06,74.06,70.27,74.61,74.61,74.6,75.54,74.11,74.23,68.3,62.97,73.71,73.74,73.31,73.78,73.31,74.04,73.56,79.05]
["Acidithiobacillus thiooxidans CLST",97.19,97.58,96.59,97.19,97.18,"*",98.35,97.57,97.15,97.16,96.35,97.16,68.37,68.61,68.22,68.47,68.37,68.22,68.44,74.38,74.38,70.27,74.69,74.75,74.73,75.74,74.45,74.48,68.35,63.14,72.97,73.44,72.91,73.3,72.91,73.03,73.37,78]
["Acidithiobacillus thiooxidans Licanantay",96.71,96.94,96.73,96.74,96.72,98.26,"*",96.96,96.73,96.72,96.4,96.63,68.63,68.85,68.59,68.81,68.62,68.6,68.64,74.2,74.2,70.27,74.76,74.9,74.82,75.01,74.61,74.53,68.39,63,73.22,73.87,73.14,74.03,73.14,73.47,73.74,78.4]
["Acidithiobacillus thiooxidans GD1-3",97.87,99.93,96.41,97.86,97.86,97.13,96.34,"*",97.84,97.87,96,97.74,69.22,68.93,69.02,69.4,69.22,68.85,69.21,73.77,73.77,73.9,73.82,73.83,73.75,74.36,74.01,74,68.23,62.93,73.25,73.22,73,73.66,73,73.7,73.43,78.81]
["Acidithiobacillus thiooxidans BY-02",99.9,98.16,96.65,99.92,99.96,97.08,96.64,98.17,"*",99.96,96.43,99.82,69.14,68.84,68.91,69.4,69.14,68.76,69.17,74.03,74.03,74.23,74.5,74.53,74.53,75,73.92,74.22,68.31,63.34,73.24,73.27,73.3,73.69,72.95,73.61,73.42,79.06]
["Acidithiobacillus thiooxidans DMC",99.89,98.1,96.64,99.92,99.93,97.06,96.63,98.11,99.93,"*",96.31,99.86,68.96,69,68.75,69.26,68.92,68.94,68.94,74.27,74.27,74.73,74.68,74.65,74.63,75.71,74.34,74.4,68.31,63.16,73.72,73.79,73.28,73.89,73.28,73.96,73.69,79.12]
["Acidithiobacillus thiooxidans ATCC 19377",97.47,97.16,97.29,97.44,97.46,97.34,97.41,97.16,97.47,97.46,"*",97.44,68.96,68.96,68.69,69.15,68.96,68.78,68.95,74.24,74.24,74.61,74.86,74.87,74.86,75.64,74.31,74.43,68.41,62.84,73.65,73.68,73.41,73.8,73.3,73.93,73.61,79.14]
["Acidithiobacillus thiooxidans A01",99.87,98.11,96.69,99.9,99.91,97.06,96.57,98.12,99.9,99.92,96.38,"*",68.75,68.83,68.53,68.73,68.75,68.48,68.74,73.9,73.9,74.24,74.49,74.55,74.54,74.4,74.18,74.46,68.38,63.61,73.22,73.69,73.08,73.99,73.41,73.37,73.6,78.46]
["Acidithiobacillus caldus DX",68.68,69.08,68.38,68.69,68.68,68.43,68.32,69.06,69.08,68.73,68.73,68.56,"*",97.93,98.22,97.75,99.93,97.65,99.96,70.14,70.27,70.4,70.23,70.26,70.25,70.6,70.01,70.1,70.39,63.38,69.45,69.68,69.51,69.8,69.51,70.17,69.89,69.06]
["Acidithiobacillus caldus S1",69,69.23,68.93,69,69.02,68.74,68.89,69.25,69.23,69.04,69.05,68.94,98.25,"*",98.09,97.74,98.24,98.04,98.22,70.42,70.64,70.83,70.57,70.56,70.53,71.07,70.33,70.46,71.1,63.42,69.82,69.88,69.85,70.18,69.85,70.11,70.19,69.23]
["Acidithiobacillus caldus ATCC 51756",68.62,69.01,68.57,68.65,68.68,68.39,68.55,69.05,69.01,68.67,68.68,68.62,98.56,98.06,"*",97.85,98.53,98.21,98.49,69.94,69.83,70.46,69.96,69.94,69.89,70.68,69.8,69.78,70.16,63.31,69.44,69.43,69.35,69.54,69.35,69.96,69.59,68.97]
["Acidithiobacillus caldus SM-1",69.5,70.42,69.53,69.63,69.62,68.78,69.15,70.49,70.41,69.71,69.66,69.12,97.75,97.74,97.85,"*",97.79,98.97,97.75,70.07,70.05,70.39,70.91,70.91,70.72,71.25,69.97,70.33,70.45,63.35,69.76,69.93,69.7,70.02,69.7,70.91,70.15,69.53]
["Acidithiobacillus caldus ZJ",68.75,69.14,68.44,68.75,68.78,68.44,68.45,69.15,69.14,68.79,68.76,68.62,99.92,97.9,98.19,98,"*",97.63,99.94,70.4,70.53,70.58,70.41,70.75,70.42,71.08,70,70.25,70.75,63.06,69.5,69.75,69.48,69.48,69.82,70.19,70.01,69.53]
["Acidithiobacillus caldus MTH-04",69.75,70.34,69.87,69.87,69.89,68.81,69.39,70.42,70.34,69.95,69.87,69.25,98.09,98.16,98.38,99.57,98.15,"*",98.1,69.76,69.69,70.2,69.88,70.41,69.93,70.26,69.63,69.62,70.59,62.94,69.83,69.91,69.64,69.64,69.84,70.85,69.93,69.53]
["Acidithiobacillus caldus ZBY",68.84,69.23,68.51,68.86,68.85,68.42,68.41,69.24,69.23,68.86,68.89,68.68,99.93,97.99,98.24,98.01,99.91,97.72,"*",70.41,70.54,70.64,70.47,70.79,70.43,71.11,70.03,70.28,70.78,62.97,69.68,70.03,69.7,69.7,70.07,70.44,70.02,69.53]
["Acidithiobacillus ferrooxidans ATCC 23270",74.74,74.7,74.26,70.54,74.76,74.75,74.54,74.71,74.8,74.76,74.57,74.57,70.4,70.46,69.95,70.34,70.4,69.76,70.41,"*",99.67,98.98,99.51,99.52,99.51,98.62,94.52,94.49,68.86,63.12,83.28,83.84,83.53,86.62,86.46,83.86,86.6,73.82]
["Acidithiobacillus ferrooxidans ATCC 53993",74.74,74.7,74.26,70.54,74.76,74.75,74.54,74.71,74.8,74.76,74.57,74.57,70.53,70.33,69.64,70.33,70.53,69.69,70.54,99.64,"*",99.46,99.49,99.49,99.5,98.69,94.54,94.4,69.14,63.11,83.47,83.88,83.56,86.72,86.55,83.86,86.63,73.82]
["Acidithiobacillus sp. GGI-221",70.62,70.65,70.27,70.51,70.75,70.99,71.23,71.47,70.62,70.62,70.62,70.62,70.62,70.65,70.27,70.51,70.58,70.2,70.64,98.51,98.91,"*",98.41,98.44,98.43,96.53,94.18,93.62,69.45,63.92,83.3,83.77,83.52,87.03,87.34,83.71,87.32,73.95]
["Acidithiobacillus ferrooxidans Hel18",74.91,74.92,74.34,70.47,74.88,74.94,74.95,74.34,74.94,74.92,74.9,74.51,70.43,70.27,69.78,70.51,70.41,69.88,70.47,99.04,99.24,98.48,"*",99.96,99.99,97.73,94.19,93.93,68.87,62.69,83.42,83.88,83.56,86.88,86.63,83.65,86.84,73.78]
["Acidithiobacillus ferrooxidans CCM 4253",75.18,75.33,74.76,70.79,75.19,75.3,75.15,74.76,75.37,75.23,75.17,74.87,70.74,70.67,70.09,71.09,70.75,70.41,70.79,98.93,99.11,98.33,99.97,"*",99.97,97.52,94.1,93.95,69.01,63.37,83.48,83.8,83.61,86.9,86.63,83.96,86.82,74.23]
["Acidithiobacillus ferrooxidans YQH-1",74.77,74.81,74.04,70.43,74.74,74.79,74.82,74.04,74.85,74.78,74.7,74.22,70.38,70.28,69.74,70.52,70.42,69.93,70.43,99.1,99.16,98.52,99.97,99.96,"*",97.64,94.17,93.79,68.86,63.28,83.36,83.93,83.54,86.87,86.61,83.65,86.86,73.56]
["Acidithiobacillus ferrooxidans DLC-5",75.14,74.87,74.15,71.11,75.13,75.15,74.57,74.15,74.89,75.16,75.13,74.26,71.11,70.86,70.35,71.48,71.08,70.26,71.11,97.49,97.7,96.46,97.09,97.1,97.09,"*",92.99,92.95,69.75,63.59,83.37,84.34,83.45,88.21,87.83,83.99,87.77,73.93]
["Acidithiobacillus ferrooxidans IO-2C",74.26,74.21,74.21,70.03,74.27,74.26,74.65,74.21,74.23,74.29,74.26,74.03,69.98,70.21,69.76,69.87,70,69.63,70.03,94.81,94.88,95.1,94.76,94.79,94.8,94.21,"*",98.92,69.1,63.37,83.17,83.25,83.57,86.39,86.57,83.61,86.69,73.53]
["Acidithiobacillus ferridurans JCM 18981",74.44,74.49,74.13,70.28,74.43,74.46,74.42,74.13,74.5,74.46,74.45,74.32,70.27,70.16,69.61,70.26,70.25,69.62,70.28,94,94.11,93.85,93.83,93.83,93.83,93.56,98.18,"*",69.03,62.98,83.31,83.85,83.59,86.97,86.7,83.52,86.78,73.51]
["Acidithiobacillus sp. UBA2486",68.39,68.48,68.48,70.78,68.44,68.44,68.41,68.48,68.49,68.41,68.41,68.5,70.76,70.9,70.49,70.66,70.75,70.59,70.78,69.08,69.08,69.58,69.14,69.13,69.11,70.04,69.1,69.09,"*",63.68,68.77,69.03,68.79,84.65,84.59,68.89,85.11,68.75]
["Acidithiobacillus sp. NORP59",62.87,62.91,62.82,62.97,62.89,62.86,62.9,62.82,62.92,62.86,62.88,63.02,62.95,63.16,62.91,62.86,63.06,62.94,62.97,62.97,62.96,63.24,62.96,62.94,62.96,63.03,62.91,62.94,63.37,"*",62.95,63.14,63.01,79.05,79.05,62.82,79.05,62.83]
["Acidithiobacillus ferrivorans YL15",69.62,69.7,69.26,69.95,73.71,72.97,73.22,73.25,73.24,73.72,73.65,73.22,69.62,69.7,69.26,69.95,69.62,69.36,69.65,83.4,83.54,83.52,83.54,83.55,83.51,83.92,83.26,83.43,68.66,63.37,"*",96.28,97.99,96.46,97.99,91.87,92.1,73.19]
["Acidithiobacillus ferrivorans SS3",70.16,69.9,69.33,70.44,73.74,73.44,73.87,73.22,73.27,73.79,73.68,73.69,70.16,69.9,69.33,70.44,70.15,69.4,70.17,null,83.54,84.06,83.54,83.55,83.51,83.92,83.26,83.43,68.66,63.37,96.02,"*",96.39,99.47,96.39,91.77,92.59,73.19]
["Acidithiobacillus ferrivorans PRJEB5721",70,69.81,69.5,70.39,73.31,72.91,73.14,73,73.3,73.28,73.41,73.08,70.16,69.9,69.33,70.44,69.93,69.5,70,86.46,86.55,83.75,86.62,86.62,86.6,87.85,86.57,86.7,84.59,63.37,97.57,96,"*",96.1,100,91.41,91.9,73.08]
["Acidithiobacillus ferrivorans PQ33",69.82,70.04,69.47,70.33,73.93,73.53,73.82,73.7,73.71,73.91,73.9,73.96,70.16,69.9,69.33,70.44,69.82,69.4,69.85,86.62,86.72,87.03,86.88,86.9,86.87,88.22,86.39,86.95,84.65,63.37,96,98.67,96,"*",96,91.43,91.93,73.37]
["Acidithiobacillus ferrivorans CF27",70,69.81,69.5,70.39,74,73.7,73.19,73.55,73.52,73.97,73.98,73.84,70.16,69.9,69.33,70.44,69.93,69.5,70,86.46,86.55,87.33,86.62,86.62,86.6,87.85,86.57,86.7,84.59,63.37,97.57,95.94,100,96.1,"*",91.41,91.9,73.14]
["Acidithiobacillus ferrooxidans BY0502",70.66,70.06,69.98,71.13,74.04,73.43,73.46,74,73.98,74.04,74.05,73.45,70.66,70.06,69.98,71.13,70.68,69.96,70.66,86.46,86.55,84.07,86.62,86.62,86.6,87.85,86.57,86.7,84.59,63.37,91.78,91.7,91.85,91.96,91.85,"*",97.84,73.14]
["Acidithiobacillus ferrivorans 21-59-9",70.25,70.39,69.95,70.44,73.53,73.64,73.43,73.55,73.54,73.49,73.5,73.43,70.16,69.9,69.33,70.44,70.22,69.95,70.29,86.59,86.63,87.32,86.84,86.83,86.86,87.79,86.69,86.78,85.11,63.29,89.79,90.4,90.04,90.27,90.04,96.5,"*",73.19]
["Acidithiobacillus sp. SH",79.71,79.55,79.14,79.78,79.66,78.69,79.01,79.6,79.72,79.83,78.87,79.29,69.06,69.23,68.97,69.53,69.5,69.5,69.53,73.82,73.82,73.95,73.78,74.23,73.56,73.93,73.53,73.51,68.75,62.83,73.19,73.19,73.08,73.37,73.14,73.14,73.19,"*"]