This PDF file includes:

• Fig. S1. MmSPO16 has a conserved XPF-like domain.
• Fig. S2. Localization of SPO16 and generation of knockout mice.
• Fig. S3. SPO16 deletion leads to massive germline loss.
• Fig. S4. Insufficient meiotic prophase progression in Spo16^−/− testes.
• Fig. S5. Insufficient meiotic recombination in SPO16-deleted spermatocytes and oocytes.
• Fig. S6. Detection of RPA complex in WT and SPO16-deleted spermatocytes.
• Table S1. Genes specifically expressed in meiotic prophase I.
• Table S2. Homology of MmSPO16 to known proteins.
• Table S3. Primer sequences.
• Table S4. Antibody information.

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