The PDF file includes:

• Fig. S1. Figure of merit that compares our work and previous work by others.
• Fig. S2. Complete schematic of the FT-CARS flow cytometer.
• Fig. S3. Digital signal processing.
• Fig. S4. Structure of the acoustofluidic-focusing microfluidic chip.
• Fig. S5. Steps for fabricating the acoustofluidic-focusing microfluidic chip.
• Fig. S6. Stability of the FT-CARS flow cytometer.
• Fig. S7. Stability of the FT-CARS spectrometer.
• Fig. S8. Image of an E. gracilis cell under a conventional optical microscope.
• Fig. S9. Images of H. lacustris cells under the nitrogen deficiency stress obtained by a conventional optical microscope.
• Fig. S10. Raman spectra obtained by FT-CARS and conventional spontaneous Raman spectroscopy.
• Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mp4 format). High-speed imaging and FT-CARS flow cytometry of fast-flowing polymer beads of multiple species.
• Movie S2 (.mp4 format). High-speed imaging and FT-CARS flow cytometry of fast-flowing E. gracilis cells.