**** Brookhaven Instruments Corp.**** ZetaPlus Particle Sizing Software Version 5.23 Sample Identification:liposomes-ACZ nanoemuls-1-3-2018 (Combined) Operator Identification: sherif Notes: water Measurement Date: Mar 1, 2018 Measurement Time: 17:36:06 Batch: 0 **** Measurement Parameters **** Temperature = 25.0 Suspension = Water Viscosity = 0.890 cp Ref.Index Fluid = 1.330 Angle = 90.00 Wavelength = 658.0 nm Runs Completed = 5 Run Duration = 00:00:15 Total Elapsed Time = 00:01:15 Average Count Rate = 482.1 kcps Ref.Index Real = 1.590 Ref.Index Imag = 0.000 **** Measurement Results **** liposomes-ACZ nanoemuls-1-3-2018 (Combined) Effective Diameter: 229.3 Polydispersity: 0.173 Baseline Index: 3.9 Elapsed Time = 00:01:15 Run Eff. Diam. (nm) Half Width (nm) Polydispersity Baseline Index --------------------------------------------------------------------------- 1 231.5 109.5 0.224 1.3 2 223.0 91.1 0.167 0.0 3 232.0 105.5 0.207 6.5 4 226.2 100.6 0.198 7.4 5 230.5 82.5 0.128 0.4 --------------------------------------------------------------------------- Mean 228.6 97.9 0.185 3.1 Std.Error 1.7 4.9 0.017 1.6 Combined 229.3 95.4 0.173 3.9 **** Lognormal Size Distribution Results **** GSD: 1.491 d(nm) G(d) C(d) | d(nm) G(d) C(d) | d(nm) G(d) C(d) -------------------------------------------------------------- 56.3 26 5 | 95.2 97 40 | 135.0 80 75 64.5 44 10 | 99.8 99 45 | 143.9 70 80 70.8 58 15 | 104.7 100 50 | 154.8 58 85 76.2 70 20 | 109.8 99 55 | 169.9 44 90 81.2 80 25 | 115.2 97 60 | 194.8 26 95 85.9 87 30 | 121.1 93 65 | 90.5 93 35 | 127.6 87 70 | **** Multimodal Size Distribution Results **** Mean Diameter: 273.863 Relative Variance: 0.121 Skew: -0.930 d(nm) G(d) C(d) | d(nm) G(d) C(d) | d(nm) G(d) C(d) -------------------------------------------------------------- 92.3 0 0 | 159.8 0 27 | 276.5 0 27 97.0 0 0 | 167.9 0 27 | 290.6 17 31 102.0 0 0 | 176.5 0 27 | 305.5 55 42 107.2 9 2 | 185.5 0 27 | 321.1 97 62 112.7 27 8 | 195.0 0 27 | 337.5 100 83 118.5 42 16 | 205.0 0 27 | 354.8 62 96 124.5 35 24 | 215.5 0 27 | 372.9 20 100 130.9 16 27 | 226.5 0 27 | 392.0 0 100 137.6 0 27 | 238.1 0 27 | 412.0 0 100 144.6 0 27 | 250.3 0 27 | 433.1 0 100 152.0 0 27 | 263.1 0 27 | 455.3 0 100 **** Lognormal Size Distribution Results/Spreadsheet Format **** 56.3, 26, 5 64.5, 44, 10 70.8, 58, 15 76.2, 70, 20 81.2, 80, 25 85.9, 87, 30 90.5, 93, 35 95.2, 97, 40 99.8, 99, 45 104.7,100, 50 109.8, 99, 55 115.2, 97, 60 121.1, 93, 65 127.6, 87, 70 135.0, 80, 75 143.9, 70, 80 154.8, 58, 85 169.9, 44, 90 194.8, 26, 95 **** Multimodal Size Distribution Results/Spreadsheet Format **** 92.3, 0, 0 97.0, 0, 0 102.0, 0, 0 107.2, 9, 2 112.7, 27, 8 118.5, 42, 16 124.5, 35, 24 130.9, 16, 27 137.6, 0, 27 144.6, 0, 27 152.0, 0, 27 159.8, 0, 27 167.9, 0, 27 176.5, 0, 27 185.5, 0, 27 195.0, 0, 27 205.0, 0, 27 215.5, 0, 27 226.5, 0, 27 238.1, 0, 27 250.3, 0, 27 263.1, 0, 27 276.5, 0, 27 290.6, 17, 31 305.5, 55, 42 321.1, 97, 62 337.5,100, 83 354.8, 62, 96 372.9, 20,100 392.0, 0,100 412.0, 0,100 433.1, 0,100 455.3, 0,100