Three-phase liquid extraction: a simple and fast method for lipidomic workflows

Supplemental Data

• Supporting information on Lippy (.pdf, 89 KB) - A database of known lipid structures, fragmentation patterns, and neutral losses was created in silico using Lipid MAPS nomenclature. Lipids were identified by matching the observed precursor/fragment mass pairs with entries in the database.
• Supplemental Table S1 (.pdf, 202 KB) - Lipid composition of SPLASH LipidoMix, which contains a mixture of all major lipid classes found in biological samples.
• Supplemental table S2 (.pdf, 206 KB) - Fatty acids monitored by single ion monitoring (SIM) in the GC-MS fatty acid profiling technique.
• Supplemental figure S2 (.pdf, 203 KB) - Extracted ion chromatogram of PE(38:4) and PC(36:3) species found in bovine liver extracts. The data was obtained by LC-MS phospholipid profile. The lipids were extracted by Bligh/Dyer (BD) and three phase liquid extraction (3PLE).escription of lippy functions
• Supplemental Figure S1 (.pdf, 243 KB) - Bovine liver extract most abundant, A) neutral lipids and B) phospholipids obtained by direct-infusion MS/MSALL.