Cyclodextrin triggers MCOLN1-dependent endo-lysosome secretion in Niemann-Pick type C cells

Supplemental Data

• Supplemental_Figure S1 to S5 (.pdf, 6.3 MB) - Supplemental Fig S1 (A) NPC1 KD (with or without HPCD). (B) Dose response of HPCD treatment (filipin staining and oleic acid incorporation in CE). Supplemental Fig S2. FIB-SEM analysis of cells treated with/without HPCD. Supplemental Fig S3. (A) Quantification of mRNA after KD. (B) Levels of MCOLN1 protein with/without KD or HPCD. (D) Levels of MCOLN1 mRNA with/without HPCD Supplemental Fig S4. Tubulin analysis by western blotting (equal loading controls in Fig 6). Supplemental Fig S5. LAMP1 distribution and quantification in NPC1 and NPC2 KO cells with or without HPCD treatment.