Northern Blotting Name Orientation Sequence dme-miR-184-3p (standard) sense UGGACGGAGAACUGAUAAGGGC dme-miR-14-3p (standard) sense UCAGUCUUUUUCUCUCUCCUAU dme-miR-7-5p (standard) sense UGGAAGACUAGUGAUUUUGUUGU anti-dme-miR-184-3p (probe) rev-comp GCCCTTATCAGTTCTCCGTCCA anti-dme-miR-14-3p (probe) rev-comp AAAAAATAGGAGAGAGAAAAAGACTGAAAAAA anti-dme-miR-7-5p (probe) rev-comp ACAACAAAATCACTAGTCTTCCA anti-2SrRNA (probe) rev-comp TACAACCCTCAACCATATGTAGTCCAAGCA dsRNA mediated knockdown eGFP fw TAATACGACTCACTATAGGGACGTAAACGGCCACAAGTTC eGFP rv TAATACGACTCACTATAGGGTGCTCAGGTAGTGGTTGTCG AGO1 fw TAATACGACTCACTATAGGGCGAAAGGTGAACCGTGAGAT AGO1 rv TAATACGACTCACTATAGGGTGAGCATCATCTTCCACTGC real-time PCR primer AGO1 fw TGTGGGACGACAATCACTTT AGO1 rv CAGATGATATCTGGCACGGA b-Tub84B fw TGGGCCCGTCTGGACCACAA b-Tub84B rv TCGCCGTCACCGGAGTCCAT HITSCLIP/PARCLIP 5'Adapter GUUCAGAGUUCUACAGUCCGACGAUC-NNNN 3'Adapter 5'App-NNNNTGGAATTCTCGGGTGCCAAGG-3'InvT Reverse transcription rv GCCTTGGCACCCGAGAATTCCA Forwards primer (FP) fw AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGA Reverse Indexing Primer (RPI1*) rv CAAGCAGAAGACGGCATACGAGATCGTGAT*GTGACTGGAGTTCCTTGGCACCCGAGAATTCCA *Other Index RPI 2-48 can be found on Illumina webpage ÒIllumina Adapter SequencesÓ RNA size marker 19nt CGUACGCGGGUUUAAACGA 24nt CGUACGCGGAAUAGUUUAAACUGU 35nt CUCAUCUUGGUCGUACGCGGAAUAGUUUAAACUGU Small RNA-seq library cloning anti-2SrRNA blocking oligo rv TACAACCCTCAACCATATGTAGTCCAAGCA/3SpC3/_3Õ Ribosomal footprinting and in-house smallRNA-seq 5'Adapter GUUCAGAGUUCUACAGUCCGACGAUC-NNNN 3'Adapter 5'App-NNNNTGGAATTCTCGGGTGCCAAGG-3'InvT SQRC-ERDN spike-in information Name Sequence molecules/ul * NSPK_RNA_1 TGCTATCATATCACAGTACGCGAGC 5757292 NSPK_RNA_2 ATCGTCTCGTCATCTCATATCTACA 73286344 NSPK_RNA_3 GATCATCGACACGTATATGCTAGAC 9407006622 NSPK_RNA_4 GCTCTACACTCTACTCGTCAGCTGT 1092697467 NSPK_RNA_6 AGTGTACATGAGCTATCTGACTACA 47523292166 NSPK_RNA_7 GAGCTATAGTCATCACATACACGTC 42252917626 NSPK_RNA_8 CGCATCAGTCGTCATCTAGATACAG 1432436499 NSPK_RNA_9 GCAGCTATCTCGCGTCATACACATA 226993393 NSPK_RNA_10 CTGATGATAGATACGCGCACACAGT 12366167843 NSPK_RNA_11 TACGCGATACTATATGATGAGACAC 49591625651 NSPK_RNA_12 CGATGCTATAGACTCTCACGTGATG 4531488342 NSPK_RNA_13 TATGCATATGATCACGAGACTCAGT 424262296 NSPK_RNA_14 CTCGACGTAGACTATCATGACTCAC 3992706081 NSPK_RNA_15 TAGATGAGATACTGATAGCGACGTA 448484998 NSPK_RNA_16 GTCAGCATCGTCGTCATACATCATA 10568572965 NSPK_RNA_17 TGTAGTATCGTGAGCTCTAGTGTCG 25524975901 NSPK_RNA_18 ACTCATCTACGTACGCATCTAGTCT 7114071 NSPK_RNA_19 ATATGTCGTCGCTATACTCTGATCG 228155917 NSPK_RNA_20 GTAGTAGATAGATGTAGCGTAGAGA 54631325 SSPK_RNA_10 GATCGAGCAG SSPK_RNA_16 CACGCACGCTCAGATA SSPK_RNA_19 TCGATACTCACGTACAGCA SSPK_RNA_22 CATACTAGAGTCGCAGCTATCG SSPK_RNA_25 CTCGCTCAGATATACTCGACTCACT SSPK_RNA_28 AGTACTAGTCACACGTAGCGATCACAGA SSPK_RNA_34 ATCTCAGACACTCGCAGACTATACTCTATCTCAG SSPK_RNA_40 ATGTCACTATAGTCACAGACAGCATCACACTCTATACTCT SSPK_RNA_50 GTCACAGATATCGTATAGCTACTCTCAGTACGCAGCAGACATCGATCATA SSPK_RNA_60 TAGCACAGTGACATAGACTCATAGATCGAGATGCTCTGATGTACGTGTACGCAGACACTA SSPK_RNA_70 TCTAGTACTATGTATATGATATCACACTCAGCACTACGTACTCATAGCTATGCTACACTATGCACGCGAT *Originally: add 2µl to 5µg of total RNA; Here: 2µl of a 1:2.5 dilution to 2µg total RNA