This PDF file includes:

• Table S1. The list of antibodies used in this study.
• Table S2. Primer information for quantitative real-time fluorescence PCR analysis of expression of target genes.
• Fig. S1. Specificity of the pT735-RalGAPα1 antibody.
• Fig. S2. Effects of HFD/fatty acids on the RalGAPα1/β complex and RalA/B activities in skeletal muscle and L6 myotubes.
• Fig. S3. Generation and characterization of the RalGAPα1^Thr735Ala knock-in mice.
• Fig. S4. Uptake of glucose and LCFAs and expression of genes for lipid metabolism in adipose tissues and primary adipocytes.
• Fig. S5. Cell surface and total CD36 in L6 myotubes.
• Fig. S6. Characterization of RalGAPα1-mKO mice.
• Fig. S7. Mapping of interaction domains on RalGAPα1 and RalGAPβ.
• Fig. S8. Expression, degradation, and interaction of RalGAPα1 and RalGAPβ in L6 myotubes expressing β-blockatide.

Download PDF