The PDF file includes:

• Supplementary Text
• Fig. S1. Single-particle cryo-EM map quality.
• Fig. S2. Single-particle cryo-EM of HDH.
• Fig. S3. Details of the assembly factor Kustc1130.
• Fig. S4. Electrostatics of HDH complex.
• Fig. S5. Central cavity in HDH trimers.
• Fig. S6. Stereo figure showing the quality of the crystallographic electron density map.
• Fig. S7. Structure of 30-mer HDH particles.
• Fig. S8. Interactions in HDH assemblies.
• Table S1. Data collection and refinement statistics.
• Legend for movie S1
• References (38–48)

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mp4 format). Heme network in the HDH complex.