LOCUS pOSY028 9225 bp DNA circular UNA 04-FEB-2019 DEFINITION pLVX SFFV PIF(SSA) HA1.7 TCRβ IRES puro. FEATURES Location/Qualifiers LTR 1..635 /label="5' LTR" misc_signal 685..822 /Name="Psi packaging signal" /label="Ψ" misc_feature 1243..1597 /Name="Rev response element" /label="RRE" misc_feature 2028..2151 /Name="central polypurine tract" /label="cPPT" promoter 2185..2627 /label="SFFV promoter" sig_peptide 2667..2723 /label="IgH-SP" CDS 2724..3023 /label="PIF(SSA)" CDS 3024..3047 /label="TCRβ linker" CDS 3048..3914 /label="HA1.7 TCRβ" misc_feature 3927..4502 /Name="internal ribosomal entry site" /Origin="Encephalomyocarditis virus" /label="IRES" CDS 4534..5133 /Name="puromycin N-acetyl-transferase" /label="pac" misc_feature 5147..5738 /Name="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5942..6576 /label="3' LTR" rep_origin complement(7048..7718) /label="pUC origin" CDS complement(7864..8724) /Name="beta-lactamase" /label="bla" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgattgaaag accccacctg taggtttggc aagctagctg 2221 cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag aagttcagat 2281 caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg cggtgagcag 2341 tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc ccggcccgag 2401 gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta agacccatca 2461 gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg aattaaccaa 2521 tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc 2581 acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccgga tctatttccg 2641 gtgaattcct cgagactagt gccaccatgg gatggagctg tatcatgctc ttcttggcag 2701 caacagctac aggtgtccac tccatgatgt tcttaccaac cgattactcg agcaggttaa 2761 gcgatcaaga gtatatggag cttgtgtttg agaatggcca gattcttgca aagggccaaa 2821 gatccaacgt tgctctgcat aatcaacgta ccaaatcgat catggatttg tatgaggcag 2881 agtataacga ggatttcatg aagagtatca tccatggtgg tggtggtgcc atcacaaatc 2941 tcggggacac gcaggttgtt ccacaaagtc atgttgctgc tgcccatgaa acaaacatgt 3001 tggaaagcaa taaacatgtt gacctcgacg gttcaggtgg agatgtgaaa gtaacccaga 3061 gctcgagata tctagtcaaa aggacgggag agaaagtttt tctggaatgt gtccaggata 3121 tggaccatga aaatatgttc tggtatcgac aagacccagg tctggggcta cggctgatct 3181 atttctcata tgatgttaaa atgaaagaaa aaggagatat tcctgagggg tacagtgtct 3241 ctagagagaa gaaggagcgc ttctccctga ttctggagtc cgccagcacc aaccagacat 3301 ctatgtacct ctgtgccagc agttcgacag ggttgcccta tggctacacc ttcggttcgg 3361 ggaccaggtt aaccgttgta gaggacctga acaaggtgtt cccacccgag gtcgctgtgt 3421 ttgagccatc agaagcagag atctcccaca cccaaaaggc cacactggtg tgcctggcca 3481 caggcttctt ccccgaccac gtggagctga gctggtgggt gaatgggaag gaggtgcaca 3541 gtggggtcag cacagacccg cagcccctca aggagcagcc cgccctcaat gactccagat 3601 actgcctgag cagccgcctg agggtctcgg ccaccttctg gcagaacccc cgcaaccact 3661 tccgctgtca agtccagttc tacgggctct cggagaatga cgagtggacc caggataggg 3721 ccaaacccgt cacccagatc gtcagcgccg aggcctgggg tagagcagac tgtggcttta 3781 cctcggtgtc ctaccagcaa ggggtcctgt ctgccaccat cctctatgag atcctgctag 3841 ggaaggccac cctgtatgct gtgctggtca gcgcccttgt gttgatggcc atggtcaaga 3901 gaaaggattt ctgaggatcc aattccgccc ctctccctcc ccccccccta acgttactgg 3961 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt 4021 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc 4081 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc 4141 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg 4201 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc 4261 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa 4321 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg 4381 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa 4441 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata 4501 agcttgccac aacccacaag gagacgacct tccatgaccg agtacaagcc cacggtgcgc 4561 ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac 4621 taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg 4681 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 4741 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 4801 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 4861 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 4921 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 4981 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 5041 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 5101 acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtc tggaacaatc aacctctgga 5161 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 5221 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 5281 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 5341 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 5401 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 5461 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 5521 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 5581 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 5641 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 5701 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac 5761 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 5821 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 5881 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga 5941 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 6001 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 6061 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 6121 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 6181 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 6241 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 6301 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 6361 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 6421 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6481 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6541 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 6601 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta 6661 gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 6721 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 6781 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 6841 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 6901 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 6961 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7021 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 7081 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7141 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7201 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7261 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7321 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7381 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7441 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7501 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 7561 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7621 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7681 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7741 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 7801 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 7861 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 7921 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 7981 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 8041 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 8101 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8161 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8221 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8281 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8341 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8401 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8461 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8521 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8581 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8641 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8701 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 8761 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 8821 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact 8881 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 8941 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 9001 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat 9061 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg 9121 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttt 9181 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt //