LOCUS pOSY063 5989 bp DNA circular UNA 04-FEB-2019 DEFINITION pMAL tac MBP PIF(A) His(6). FEATURES Location/Qualifiers misc_feature 72..1163 /label="lacI" promoter 1407..1435 /label="tac promoter" CDS 1528..2628 /label="MBP" CDS 2629..2676 /label="MBP linker" CDS 2677..2700 /label="HRV3C site" CDS 2701..2724 /label="TCRβ linker" CDS 2725..3024 /label="PIF(A)" CDS 3025..3042 /label="His(6)" terminator 3253..3280 /label="rrnB_T2 terminator" promoter 3329..3357 /label="bla promoter" CDS 3399..4259 /Name="beta-lactamase" /label="bla" rep_origin complement(4337..4937) /label="ColE1 origin" gene complement(5305..5496) /label="ROP" ORIGIN 1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatttcggta gtgggatacg 901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag 1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg 1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg 1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt 1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga 1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga 1501 gcaattgacc aacaaggacc atagattatg aaaatcgaag aaggtaaact ggtaatctgg 1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat 1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt 1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac 1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat 1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt 1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa 1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac 1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag 2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg 2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac 2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg 2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc 2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt 2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg 2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag 2461 ttggtgaaag atccgcgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg 2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc 2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg 2641 aacaacaaca acaataacaa taacaacaac ctcgggctgg aagtgctgtt tcagggcccg 2701 ctcgacggtt caggtggaga tgtgatgatg ttcttaccaa ccgattattg ttgcaggtta 2761 agcgatcaag agtatatgga gcttgtgttt gagaatggcc agattcttgc aaagggccaa 2821 agatccaacg ttgctctgca taatcaacgt accaaatcga tcatggattt gtatgaggca 2881 gagtataacg aggatttcat gaagagtatc atccatggtg gtggtggtgc catcacaaat 2941 ctcggggaca cgcaggttgt tccacaaagt catgttgctg ctgcccatga aacaaacatg 3001 ttggaaagca ataaacatgt tgaccaccat catcaccatc actaagaatt ccctgcaggt 3061 aattaaataa gcttcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 3121 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga 3181 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc 3241 atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt 3301 cggtccgttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 3361 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 3421 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 3481 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 3541 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttc ccaatgatga 3601 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc 3661 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 3721 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 3781 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 3841 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 3901 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 3961 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 4021 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 4081 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 4141 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 4201 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 4261 tgtcagacca agtttactca tatatacttt agattgattt ccttaggact gagcgtcaac 4321 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 4381 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 4441 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 4501 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 4561 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 4621 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 4681 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 4741 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 4801 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 4861 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 4921 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 4981 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 5041 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 5101 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt 5161 tcacaccgca tataaggtgc actgtgactg ggtcatggct gcgccccgac acccgccaac 5221 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt 5281 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag 5341 gcagctgcgg taaagctcat cagcgtggtc gtgcagcgat tcacagatgt ctgcctgttc 5401 atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg 5461 ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt 5521 tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac 5581 tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat 5641 gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt 5701 aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca 5761 gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt 5821 tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg 5881 tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag 5941 gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgaaatt //