LOCUS pOSY075 9954 bp DNA circular UNA 04-FEB-2019 DEFINITION pLVX SFFV moxGFP F3 PIF(SSA) HA1.7 TCRβ IRES puro. FEATURES Location/Qualifiers LTR 1..635 /label="5' LTR" misc_signal 685..822 /Name="Psi packaging signal" /label="Ψ" misc_feature 1243..1597 /Name="Rev response element" /label="RRE" misc_feature 2028..2151 /Name="central polypurine tract" /label="cPPT" promoter 2185..2627 /label="SFFV promoter" sig_peptide 2667..2723 /label="IgH-SP" CDS 2718..3434 /label="moxGFP" CDS 3435..3452 /label="F3" CDS 3453..3752 /label="PIF(SSA)" CDS 3753..3776 /label="TCRβ linker" CDS 3777..4640 /label="HA1.7 TCRβ" misc_feature 4656..5231 /Name="internal ribosomal entry site" /Origin="Encephalomyocarditis virus" /label="IRES" CDS 5263..5862 /Name="puromycin N-acetyl-transferase" /label="pac" misc_feature 5876..6467 /Name="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6671..7305 /label="3' LTR" rep_origin complement(7777..8447) /label="pUC origin" CDS complement(8593..9453) /Name="beta-lactamase" /label="bla" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgattgaaag accccacctg taggtttggc aagctagctg 2221 cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag aagttcagat 2281 caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg cggtgagcag 2341 tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc ccggcccgag 2401 gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta agacccatca 2461 gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg aattaaccaa 2521 tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc 2581 acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccgga tctatttccg 2641 gtgaattcct cgagactagt gccaccatgg gatggagctg tatcatgctc ttcttggcag 2701 caacagctac aggtgtccac tcctccaagg gcgaggagct gttcaccggg gtggtgccca 2761 tcctggtcga gctggacggc gacgtaaacg gccacaagtt ctccgtgcgg ggcgagggcg 2821 agggcgatgc caccaacggc aagctgaccc tgaagttcat cagcaccacc ggcaagctgc 2881 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagagc ttctcccgct 2941 accccgacca catgaagcgc cacgacttct tcaagagcgc catgcccgaa ggctacgtcc 3001 aggagcgcac catctccttc aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt 3061 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 3121 gcaacatcct ggggcacaag ctggagtaca acttcaactc ccacaacgtc tatatcaccg 3181 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac gtggaggacg 3241 gctccgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 3301 tgctgcccga caaccactac ctgtccaccc agtccaagct gtccaaagac cccaacgaga 3361 agcgcgatca catggtcctt ctggaattcg tgaccgccgc cgggatcact cacggcatgg 3421 acgagctgta caagggcagc aggagaaaga gaatgatgtt cttaccaacc gattactcga 3481 gcaggttaag cgatcaagag tatatggagc ttgtgtttga gaatggccag attcttgcaa 3541 agggccaaag atccaacgtt gctctgcata atcaacgtac caaatcgatc atggatttgt 3601 atgaggcaga gtataacgag gatttcatga agagtatcat ccatggtggt ggtggtgcca 3661 tcacaaatct cggggacacg caggttgttc cacaaagtca tgttgctgct gcccatgaaa 3721 caaacatgtt ggaaagcaat aaacatgttg acctcgacgg ttcaggtgga gatgtgaaag 3781 taacccagag ctcgagatat ctagtcaaaa ggacgggaga gaaagttttt ctggaatgtg 3841 tccaggatat ggaccatgaa aatatgttct ggtatcgaca agacccaggt ctggggctac 3901 ggctgatcta tttctcatat gatgttaaaa tgaaagaaaa aggagatatt cctgaggggt 3961 acagtgtctc tagagagaag aaggagcgct tctccctgat tctggagtcc gccagcacca 4021 accagacatc tatgtacctc tgtgccagca gttcgacagg gttgccctat ggctacacct 4081 tcggttcggg gaccaggtta accgttgtag aggacctgaa caaggtgttc ccacccgagg 4141 tcgctgtgtt tgagccatca gaagcagaga tctcccacac ccaaaaggcc acactggtgt 4201 gcctggccac aggcttcttc cccgaccacg tggagctgag ctggtgggtg aatgggaagg 4261 aggtgcacag tggggtcagc acagacccgc agcccctcaa ggagcagccc gccctcaatg 4321 actccagata ctgcctgagc agccgcctga gggtctcggc caccttctgg cagaaccccc 4381 gcaaccactt ccgctgtcaa gtccagttct acgggctctc ggagaatgac gagtggaccc 4441 aggatagggc caaacccgtc acccagatcg tcagcgccga ggcctggggt agagcagact 4501 gtggctttac ctcggtgtcc taccagcaag gggtcctgtc tgccaccatc ctctatgaga 4561 tcctgctagg gaaggccacc ctgtatgctg tgctggtcag cgcccttgtg ttgatggcca 4621 tggtcaagag aaaggatttc tgaggatcca attccgcccc tctccctccc ccccccctaa 4681 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 4741 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 4801 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 4861 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 4921 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 4981 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 5041 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 5101 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc 5161 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 5221 acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga gtacaagccc 5281 acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg 5341 ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc 5401 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 5461 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 5521 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 5581 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 5641 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 5701 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 5761 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 5821 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgtct ggaacaatca 5881 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 5941 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 6001 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 6061 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 6121 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 6181 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 6241 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 6301 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 6361 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 6421 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc 6481 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct 6541 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag 6601 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 6661 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg 6721 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc 6781 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta 6841 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg 6901 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac 6961 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag 7021 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga 7081 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga 7141 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 7201 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 7261 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 7321 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg 7381 acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 7441 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 7501 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 7561 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 7621 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 7681 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 7741 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 7801 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 7861 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 7921 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 7981 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 8041 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 8101 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 8161 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 8221 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 8281 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 8341 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 8401 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 8461 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 8521 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 8581 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 8641 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 8701 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 8761 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 8821 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 8881 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 8941 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 9001 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 9061 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 9121 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 9181 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 9241 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 9301 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 9361 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 9421 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 9481 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 9541 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg 9601 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 9661 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 9721 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc 9781 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg 9841 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact 9901 tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt //