LOCUS pOSY076 9948 bp DNA circular UNA 04-FEB-2019 DEFINITION pLVX SFFV moxGFP noF PIF(SSA) HA1.7 TCRβ IRES puro. FEATURES Location/Qualifiers LTR 1..635 /label="5' LTR" misc_signal 685..822 /Name="Psi packaging signal" /label="Ψ" misc_feature 1243..1597 /Name="Rev response element" /label="RRE" misc_feature 2028..2151 /Name="central polypurine tract" /label="cPPT" promoter 2185..2627 /label="SFFV promoter" sig_peptide 2667..2723 /label="IgH-SP" CDS 2718..3434 /label="moxGFP" CDS 3435..3446 /label="noF" CDS 3447..3746 /label="PIF(SSA)" CDS 3747..3770 /label="TCRβ linker" CDS 3771..4634 /label="HA1.7 TCRβ" misc_feature 4650..5225 /Name="internal ribosomal entry site" /Origin="Encephalomyocarditis virus" /label="IRES" CDS 5257..5856 /Name="puromycin N-acetyl-transferase" /label="pac" misc_feature 5870..6461 /Name="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6665..7299 /label="3' LTR" rep_origin complement(7771..8441) /label="pUC origin" CDS complement(8587..9447) /Name="beta-lactamase" /label="bla" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgattgaaag accccacctg taggtttggc aagctagctg 2221 cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag aagttcagat 2281 caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg cggtgagcag 2341 tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc ccggcccgag 2401 gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta agacccatca 2461 gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg aattaaccaa 2521 tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc 2581 acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccgga tctatttccg 2641 gtgaattcct cgagactagt gccaccatgg gatggagctg tatcatgctc ttcttggcag 2701 caacagctac aggtgtccac tcctccaagg gcgaggagct gttcaccggg gtggtgccca 2761 tcctggtcga gctggacggc gacgtaaacg gccacaagtt ctccgtgcgg ggcgagggcg 2821 agggcgatgc caccaacggc aagctgaccc tgaagttcat cagcaccacc ggcaagctgc 2881 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagagc ttctcccgct 2941 accccgacca catgaagcgc cacgacttct tcaagagcgc catgcccgaa ggctacgtcc 3001 aggagcgcac catctccttc aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt 3061 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 3121 gcaacatcct ggggcacaag ctggagtaca acttcaactc ccacaacgtc tatatcaccg 3181 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac gtggaggacg 3241 gctccgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 3301 tgctgcccga caaccactac ctgtccaccc agtccaagct gtccaaagac cccaacgaga 3361 agcgcgatca catggtcctt ctggaattcg tgaccgccgc cgggatcact cacggcatgg 3421 acgagctgta caagggaggt tctggcatga tgttcttacc aaccgattac tcgagcaggt 3481 taagcgatca agagtatatg gagcttgtgt ttgagaatgg ccagattctt gcaaagggcc 3541 aaagatccaa cgttgctctg cataatcaac gtaccaaatc gatcatggat ttgtatgagg 3601 cagagtataa cgaggatttc atgaagagta tcatccatgg tggtggtggt gccatcacaa 3661 atctcgggga cacgcaggtt gttccacaaa gtcatgttgc tgctgcccat gaaacaaaca 3721 tgttggaaag caataaacat gttgacctcg acggttcagg tggagatgtg aaagtaaccc 3781 agagctcgag atatctagtc aaaaggacgg gagagaaagt ttttctggaa tgtgtccagg 3841 atatggacca tgaaaatatg ttctggtatc gacaagaccc aggtctgggg ctacggctga 3901 tctatttctc atatgatgtt aaaatgaaag aaaaaggaga tattcctgag gggtacagtg 3961 tctctagaga gaagaaggag cgcttctccc tgattctgga gtccgccagc accaaccaga 4021 catctatgta cctctgtgcc agcagttcga cagggttgcc ctatggctac accttcggtt 4081 cggggaccag gttaaccgtt gtagaggacc tgaacaaggt gttcccaccc gaggtcgctg 4141 tgtttgagcc atcagaagca gagatctccc acacccaaaa ggccacactg gtgtgcctgg 4201 ccacaggctt cttccccgac cacgtggagc tgagctggtg ggtgaatggg aaggaggtgc 4261 acagtggggt cagcacagac ccgcagcccc tcaaggagca gcccgccctc aatgactcca 4321 gatactgcct gagcagccgc ctgagggtct cggccacctt ctggcagaac ccccgcaacc 4381 acttccgctg tcaagtccag ttctacgggc tctcggagaa tgacgagtgg acccaggata 4441 gggccaaacc cgtcacccag atcgtcagcg ccgaggcctg gggtagagca gactgtggct 4501 ttacctcggt gtcctaccag caaggggtcc tgtctgccac catcctctat gagatcctgc 4561 tagggaaggc caccctgtat gctgtgctgg tcagcgccct tgtgttgatg gccatggtca 4621 agagaaagga tttctgagga tccaattccg cccctctccc tccccccccc ctaacgttac 4681 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat 4741 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat 4801 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga 4861 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca 4921 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac 4981 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt 5041 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca 5101 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt 5161 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg 5221 ataagcttgc cacaacccac aaggagacga ccttccatga ccgagtacaa gcccacggtg 5281 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc 5341 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag 5401 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 5461 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 5521 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 5581 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 5641 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 5701 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 5761 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 5821 cgcacctggt gcatgacccg caagcccggt gcctgaacgc gtctggaaca atcaacctct 5881 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 5941 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 6001 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 6061 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 6121 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 6181 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 6241 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc 6301 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 6361 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 6421 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga 6481 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt 6541 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct 6601 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg 6661 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac 6721 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat 6781 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag 6841 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac 6901 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc 6961 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt 7021 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc 7081 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat 7141 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 7201 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 7261 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 7321 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg 7381 ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 7441 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 7501 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 7561 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 7621 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 7681 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 7741 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 7801 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 7861 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 7921 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 7981 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 8041 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 8101 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 8161 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 8221 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 8281 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 8341 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 8401 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 8461 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 8521 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 8581 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 8641 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 8701 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 8761 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 8821 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 8881 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 8941 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 9001 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 9061 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 9121 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 9181 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 9241 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 9301 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 9361 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 9421 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 9481 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 9541 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca 9601 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 9661 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 9721 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc 9781 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct 9841 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag 9901 tttttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt //