LOCUS pOSY019 9654 bp DNA circular UNA 04-FEB-2019 DEFINITION pLVX SFFV scFv HA1.7 TCRβ IRES puro. FEATURES Location/Qualifiers LTR 1..635 /label="5' LTR" misc_signal 685..822 /Name="Psi packaging signal" /label="Ψ" misc_feature 1243..1597 /Name="Rev response element" /label="RRE" misc_feature 2028..2151 /Name="central polypurine tract" /label="cPPT" promoter 2185..2627 /label="SFFV promoter" sig_peptide 2667..2723 /label="IgH-SP" CDS 2724..3452 /name="NIP-specific scFv" /label="scFv" CDS 3453..3476 /label="TCRβ linker" CDS 3477..4343 /label="HA1.7 TCRβ" misc_feature 4356..4931 /Name="internal ribosomal entry site" /Origin="Encephalomyocarditis virus" /label="IRES" CDS 4963..5562 /Name="puromycin N-acetyl-transferase" /label="pac" misc_feature 5576..6167 /Name="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6371..7005 /label="3' LTR" rep_origin complement(7477..8147) /label="pUC origin" CDS complement(8293..9153) /Name="beta-lactamase" /label="bla" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgattgaaag accccacctg taggtttggc aagctagctg 2221 cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag aagttcagat 2281 caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg cggtgagcag 2341 tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc ccggcccgag 2401 gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta agacccatca 2461 gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg aattaaccaa 2521 tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc 2581 acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccgga tctatttccg 2641 gtgaattcct cgagactagt gccaccatgg gatggagctg tatcatgctc ttcttggcag 2701 caacagctac aggtgtccac tcccaggtcc aactgcagca gtctggggct gagcttgtga 2761 agcctggggc ttcagtgaag ctgtcctgca aggcttctgg ctacaccttc accagctact 2821 ggatgcactg ggtgaagcag aggcctggac gaggccttga gtggattgga aggattgatc 2881 ctaatagtgg tggtactaag tacaatgaga agttcaagag caaggccaca ctgactgtag 2941 acaaaccctc cagcacagcc tacatgcagc tcagcagcct gacatctgag gactctgcgg 3001 tctattattg tgcaagatac gattactacg gtagtagcta ctttgactac tggggccaag 3061 ggaccacggt caccgtctcc tcaggtggag gcggttcagg cggaggtggc tctggcggtg 3121 gcggatctca ggctgttggg acacaggaat ctgcactcac cacatcacct ggtgaaacag 3181 tcacactcac ttgtcgctca agtactgggg ctgttacaac tagtaactat gccaactggg 3241 tccaagaaaa accagatcat ttattcactg gtctaatagg tggtaccaac aaccgagctc 3301 caggtgttcc tgccagattc tcaggctccc tgattggaga caaggctgcc ctcaccatca 3361 caggggcaca gactgaggat gaggcaatat atttctgtgc tctatggtac agcaaccatt 3421 gggtgttcgg tggaggaacc aaactgactg tcctcgacgg ttcaggtgga gatgtgaaag 3481 taacccagag ctcgagatat ctagtcaaaa ggacgggaga gaaagttttt ctggaatgtg 3541 tccaggatat ggaccatgaa aatatgttct ggtatcgaca agacccaggt ctggggctac 3601 ggctgatcta tttctcatat gatgttaaaa tgaaagaaaa aggagatatt cctgaggggt 3661 acagtgtctc tagagagaag aaggagcgct tctccctgat tctggagtcc gccagcacca 3721 accagacatc tatgtacctc tgtgccagca gttcgacagg gttgccctat ggctacacct 3781 tcggttcggg gaccaggtta accgttgtag aggacctgaa caaggtgttc ccacccgagg 3841 tcgctgtgtt tgagccatca gaagcagaga tctcccacac ccaaaaggcc acactggtgt 3901 gcctggccac aggcttcttc cccgaccacg tggagctgag ctggtgggtg aatgggaagg 3961 aggtgcacag tggggtcagc acagacccgc agcccctcaa ggagcagccc gccctcaatg 4021 actccagata ctgcctgagc agccgcctga gggtctcggc caccttctgg cagaaccccc 4081 gcaaccactt ccgctgtcaa gtccagttct acgggctctc ggagaatgac gagtggaccc 4141 aggatagggc caaacccgtc acccagatcg tcagcgccga ggcctggggt agagcagact 4201 gtggctttac ctcggtgtcc taccagcaag gggtcctgtc tgccaccatc ctctatgaga 4261 tcctgctagg gaaggccacc ctgtatgctg tgctggtcag cgcccttgtg ttgatggcca 4321 tggtcaagag aaaggatttc tgaggatcca attccgcccc tctccctccc ccccccctaa 4381 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 4441 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 4501 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 4561 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 4621 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 4681 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 4741 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 4801 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc 4861 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 4921 acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga gtacaagccc 4981 acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg 5041 ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc 5101 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 5161 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 5221 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 5281 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 5341 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 5401 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 5461 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 5521 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgtct ggaacaatca 5581 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 5641 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 5701 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 5761 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 5821 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 5881 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 5941 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 6001 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 6061 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 6121 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc 6181 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct 6241 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag 6301 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 6361 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg 6421 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc 6481 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta 6541 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg 6601 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac 6661 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag 6721 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga 6781 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga 6841 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 6901 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 6961 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 7021 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg 7081 acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 7141 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 7201 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 7261 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 7321 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 7381 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 7441 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 7501 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 7561 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 7621 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 7681 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 7741 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 7801 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 7861 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 7921 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 7981 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 8041 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 8101 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 8161 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 8221 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 8281 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 8341 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 8401 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 8461 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 8521 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 8581 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 8641 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 8701 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 8761 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 8821 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 8881 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 8941 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 9001 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 9061 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 9121 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 9181 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 9241 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg 9301 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 9361 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 9421 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc 9481 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg 9541 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact 9601 tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt //