The temperature-signaling cascade in sponges involves a heat-gated cation channel, abscisic acid, and cyclic ADP-ribose -- Data Supplement - Supporting Information -- Proceedings of the National Academy of Sciences

Supporting information for Zocchi et al. (2001) Proc. Natl. Acad. Sci. USA 98 (26), 14859–14864. (10.1073/pnas.261448698)

Supporting Figure 9 Fig. 9.
Efflux of ⁸⁶Rb⁺ from A. polypoides cells. (A) Kinetics of ⁸⁶Rb⁺ efflux evoked by AA or 28°C. AA (50 m M at 14°C) or high temperature were applied starting from the fifth 10-min incubation interval. Each point represents the mean radioactivity per fraction from four different experiments (SD £ 10% for all time points). ♦, AA; ▲, 28°C; <, control 14°C. (B) Summary of the effects of K⁺ channel activators or inhibitors on ⁸⁶Rb⁺ efflux. Total radioactivity released during three consecutive 10-min incubation intervals was determined under the various conditions. Gd³⁺ (50 mM), Ba²⁺ (1 mM), La³⁺ (50 mM), or 8-bromo-cAMP (500 mM) were added to the cells at the time of AA (50 mM) or temperature (28°C) stimulation (see A). K252a (1 mM) or bupivacaine (BP, 1 mM) were added at time = zero. Control efflux was the radioactivity spontaneously released from the cells at 14°C during the same time.