A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila -- Data Supplement - Supporting Movies -- Proceedings of the National Academy of Sciences

Supporting information for Morin et al. (December 11, 2001) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.261408198.

Supporting Movie 4 Movie 4.
This movie shows the distribution of a new nuclear protein, trapped with GFP, during a syncitial division of the Drosophila embryo (line G180). The only feature deduced from the predicted protein sequence is a nuclear targeting signal. Our analysis confirms this nuclear localization of GFP, which is detected in the extrachromosomal nuclear domain (END). Time-lapse analysis shows the dynamics of this protein’s distribution during the cell cycle (compare with the chromosomal distribution of GFP in Movie 3). Note the exclusion of the signal from the equatorial plate at metaphase. (Time scale: accelerated 225 times.)