The PDF file includes:

• Fig. S1. Protein structural conservation in various domain and sex-specific regions of Dsx across insect orders.
• Legends for tables S1 to S3

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Other Supplementary Material for this manuscript includes the following:

• Table S1 (Microsoft Excel format). Species, GenBank accession numbers, and exons of dsx sequences used in this study.
• Table S2 (.txt format). Regression log file.
• Table S3 (Microsoft Excel format). Sites under episodic and pervasive positive selection in different insect groups and specific lineages.