FungiUstilaginalesUstilaginaceaeLiYing-MingShivasRoger G.LiBao-JuCaiLeiDiversity of Moesziomyces (Ustilaginales, Ustilaginomycotina) on Echinochloa and Leersia (Poaceae)MycoKeys09520195211610.3897/mycokeys.52.30461 Moesziomyces globuligerus (Berk. & Broome) Vánky, Bot. Not. 130: 135 (1977)Figure 2e–g Thecaphora globuligera Berk. & Broome, Trans. Linn. Soc. London, Bot., Ser. 2, 1: 407 (1880). — Type: AUSTRALIA, Queensland, Brisbane, on Leersiahexandra, F.M. Bailey, No. 86 (K(M) 252436, lectotype designated here, MBT 385180, not seen; K(M) 252437, syntype). [Basionym] Tolyposporium globuligerum (Berk. & Broome) Ricker, J. Mycol. 11:112 (1905). Testicularia leersiae Cornu, Ann. Sci. Nat. Bot., Sér. 6, 15: 275 (1883).Description.

Sori in some of the ovaries, often deciduous, ellipsoidal to oval, 2.5–4 × 1.5–3 mm, green at first, later brown, smooth, ruptures irregularly to reveal a granular, dark brown mass of spore balls; columella absent. Spore balls subglobose, ellipsoidal or irregular, 75–150 µm in diameter, yellowish brown, composed of up to several hundred spores that separate by moderate pressure. Spores subglobose, ovoid to irregularly polyhedral, (8–) 8.5–11 (–13) × (6–) 7–9 (–10) μm ( = 9.6 ± 1.2 × 7.9 ± 0.9 μm, n = 50), subhyaline to pale yellowish brown, attached together by multiple narrow cylindrical protuberances about 2 μm wide and 1–2 μm long; wall with irregular meshes and wings, less than 0.5 μm thick, smooth. (Based on specimen BRIP 27384).

Specimens examined.

AUSTRALIA, Queensland, Willowbank, on Leersiahexandra, 9 Mar. 1998, C. Vánky & K. Vánky leg., BRIP 27384; Queensland, Mareeba, on L.hexandra, 1 May 2004, M.D.E. Shivas & R.G. Shivas leg., BRIP 44301; Queensland, Mt Garnet, on L.hexandra, 5 May 2005, T.S. Marney & R.G. Shivas leg., BRIP 44569; Northern Territory, Darwin, on L.hexandra, 15 Apr. 2008, J. Ray, A.A. Mitchell, A.R. McTaggart & R.G. Shivas leg., BRIP 51872. CHINA, Guangxi province, on L.hexandra, Oct. 2017, R.G. Shivas, M.D.E. Shivas, Y.-M. Li, P. Zhao & X.-H. Qi leg., HMAS 248027. THAILAND, Kanchanaburi, on L.hexandra, 16 Dec. 2005, R.G. Shivas & M.D.E. Shivas leg., BRIP 47767; Chiang Mai, on L.hexandra, 26 Dec. 2005, R.G. Shivas & M.D.E. Shivas leg., BRIP 47768.

Notes.

Vánky (1986) considered that M.globuligerus was a synonym of M.bullatus based on their similar morphologies. Phylogenetic analyses support M.globuligerus as a distinct species (Fig. 1), with a teleomorph specific to the pantropical grass Leersiahexandra (Berkeley and Broome 1880). The name Testicularialeersiae (Cornu 1883), described from infected Leersiahexandra in Algeria, is likely a heterotypic synonym of M.globuligerus, but this has not been checked by molecular phylogenetic analysis. The type material of Thecaphoraglobuligera was collected circa 1878 from near the Brisbane River, Queensland, Australia by the botanist F. M. Bailey (Berkeley and Broome 1880). Original material of this specimen (F.M. Bailey, No. 86) could not be found in the Australian herbaria BRI and BRIP, where most of F.M. Bailey’s specimens are held. Two syntypes were located in K(M), of which K(M) 252436 ex C.E. Broome herbarium (BM) was selected as lectotype of T.globuligera (now M.globuligerus). The material in the second specimen, K(M) 252437 from the Berkeley herbarium, was scant (Dr Begoña Aguirre-Hudson pers. comm).

a–dMoesziomyceskimberleyensis (holotype BRIP 51843) e–gMoesziomycesglobuligerus (BRIP 27384) h–kMoesziomycesantarcticus (HMAS 60130). a, b: sori. c, d, f, j, k: spores under LM. g: spores under SEM. Scale bars: 1 mm (b, i); 10 µm (c, d, f, g, j, k).

Phylogram obtained from a ML analysis based on the ITS sequence alignment. Values above the branches represent ML bootstrap values (> 70%) from RaxML and PhyML analysis respectively. Thickened branches represent Bayesian posterior probabilities (> 0.95). The scale bar indicates 0.03 expected substitutions per site. * indicates type specimens or type strains.

VánkyK (1986) The genus Moesziomyces (Ustilaginales).Nordic Journal of Botany6: 6773. https://doi.org/10.1111/j.1756-1051.1986.tb00860.xBerkeleyMJBroomeCE (1880) . XXII. List of fungi from Brisbane, Queensland; with descriptions of new species. Transactions of the Linnean Society London, Botany 1: 399−407. https://doi.org/10.1111/j.1095-8339.1879.tb00140.xCornuM (1883) Sur quelques Ustilaginees nouvelles ou peu connues. Ann Sci Nat Bot, Ser 6 15: 269−296.