,S2,,
,Designation ,TRC# ,Sequence
,shCPS1_4,TRCN0000428716,CCGGGAACAGCCAGATGGGTTAATTCTCGAGAATTAACCCATCTGGCTGTTCTTTTTTG
,shCSP1_5,TRCN0000045443,CCGGGCCAACCCTATTATTGGGAATCTCGAGATTCCCAATAATAGGGTTGGCTTTTTG
,shASL_1,TRCN0000078308,CCGGGTGGATGTTCAAGGCAGCAAACTCGAGTTTGCTGCCTTGAACATCCACTTTTTG
,shASL_3,TRCN0000078312,CCGGGAGGACAAGGAAGCTGTGTTTCTCGAGAAACACAGCTTCCTTGTCCTCTTTTTG
,shASS1_1,TRCN0000045553,CCGGCCATCCTTTACCATGCTCATTCTCGAGAATGAGCATGGTAAAGGATGGTTTTTG
,shOTC_2,TRCN0000412388,CCGGCACAACTTCATGGTTCGAAATCTCGAGATTTCGAACCATGAAGTTGTGTTTTTTG
,shARG_2,TRCN000051022,GTTCACCAGATGAATCAGAAA
,Non-Mammalian shRNAshC002,,CCGGCAACAAGATGAAGAGCACCAACTCGAGTTGGTGCTCTTCATCTTGTTGTTTTT
,,,
,S3,,
,Primers,sequence,Roche UPL number probe
,CPS1_FOR,CAAGTTTTGCAGTGGAATCG,8
,CPS1_REV,ACTGGGTAGCCAATGGTGTC,
,b-actin_FOR,CCAACCGCGAGAAGATGA,64
,b-actin_REV,TCCATCACGATGCCAGTG,
,GAPDH_FOR,AGCCACATCGCTCAGACAC,60
,GAPDH_REV,GCCCAATACGACAAATCC,
,ASL_FOR,CAGTGGACCCCATCATGGAGA,
,ASL_REV,GGCTTTGCTGCCTTGAACATC,
,HPRT_FOR,GTGAAAAGGACCCCACGAAG,
,HPRT_REV,AGGCTTTGTATTTTGCTTTTCCA,
,,,
,,,
,S4,,
,Protein ,Clone/ID# ,Vendor
,pEGFR (Y1068) ,#3777 ,Cell Signaling 
,pERK (T202/Y204),#9101S,Cell Signaling 
,Histone H3,#96C10,Cell Signaling 
,B-actin,sc-1616,Santa Cruz Biotech. 
,CPS1,ab3682,Abcam 
,,,
,,,
,S5,,
,1,Acute Promyelocytic Leukemia ,1
,2,Anaplastic Astrocytoma,1
,3,Blast Phase Chronic Myelogenous Leukemia,1
,4,Brain Glioblastoma,3
,5,Breast Adenocarcinoma,3
,6,Breast Carcinoma,1
,7,Bronchioloalveolar Carcinoma,1
,8,Clear Cell Renal Cell Carcinoma,1
,9,Colon Adenocarcinoma,5
,10,Colon Carcinoma,2
,11,Cutaneous Melanoma,3
,12,Ductal Breast Carcinoma,1
,13,Glioblastoma,2
,14,Immunoblastic Lymphoma,1
,15,Invasive Ductal Breast Carcinoma,1
,16,Large Cell Lung Carcinoma,1
,17,Lung Adenocarcinoma,2
,18,Lung Cancer,1
,19,Melanoma,7
,20,Multiple Myeloma,1
,21,Non-Small Cell Lung Carcinoma,3
,22,Ovarian Adenocarcinoma,2
,23,Ovarian Carcinoma,4
,24,Prostate Adenocarcinoma,1
,25,Prostate Carcinoma,1
,26,Renal Carcinoma,1
,27,Renal Cell Carcinoma,6
,28,Squamous Cell Lung Carcinoma ,1
,29,T-Cell Acute Lymphoblastic Leukemia ,2