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["Read count statistics"]
["Sample name","Read count","Single, mapped %","Single, unmapped %","Paired, mapped pairs %","Paired, broken pairs %","Paired, not mapped %"]
["DRBD2_2 trimmed RNA-Seq report-1",3.9211175E7,26.94,73.06,"N/A","N/A","N/A"]
["DRBD2_3 trimmed RNA-Seq report",1.4754641E7,47.85,52.15,"N/A","N/A","N/A"]
["DRBD2_4 trimmed RNA-Seq report",1.7112744E7,47.26,52.74,"N/A","N/A","N/A"]
["Control_1 trimmed RNA-Seq report",4.723556E7,6.18,93.82,"N/A","N/A","N/A"]
["Control_3 trimmed RNA-Seq report",4.506757E7,11.32,88.68,"N/A","N/A","N/A"]
["Control_2 trimmed RNA-Seq report",4.6474498E7,16.08,83.92,"N/A","N/A","N/A"]
["DRBD2_1 trimmed-1 RNA-Seq report",3.2343987E7,23.6,76.4,"N/A","N/A","N/A"]
[]
["Single, unmapped %: >50% of the reads did not map. If the correct reference genome has been selected, this could indicate a serious problem with the sample.\n • Check that the correct reference genome and any relevant gene/mRNA tracks have been provided.\n • The mapping parameters may be too strict. Try resetting them to the default values.\n • Try mapping the un-mapped reads against possible contaminants. If the sample is contaminated, enrich for the target species before library preparation in future experiments.\n • Library preparation may have failed. Check the quality of the sample RNA."]
[]
["For paired data, there are two reads in a pair."]
[]
["Fragment counting statistics"]
["Sample name","Mapped to genes %","Mapped to intergenic %"]
["DRBD2_2 trimmed RNA-Seq report-1",28.5,71.5]
["DRBD2_3 trimmed RNA-Seq report",13.04,86.96]
["DRBD2_4 trimmed RNA-Seq report",13.06,86.94]
["Control_1 trimmed RNA-Seq report",34.92,65.08]
["Control_3 trimmed RNA-Seq report",18.36,81.64]
["Control_2 trimmed RNA-Seq report",26.24,73.76]
["DRBD2_1 trimmed-1 RNA-Seq report",26.08,73.92]
[]
["Default counting scheme ('Fragment counts'): An intact pair is counted as one, broken pairs are ignored."]
[]
["Strand specificity"]
["Sample name","Strand specific setting","Forward % of reads mapped","Reverse % of reads mapped","Ignored reads (wrong strand)","Ignored reads % (wrong strand)"]
["DRBD2_2 trimmed RNA-Seq report-1","Both",77.59,22.41,0.0,0.0]
["DRBD2_3 trimmed RNA-Seq report","Both",90.93,9.07,0.0,0.0]
["DRBD2_4 trimmed RNA-Seq report","Both",90.47,9.53,0.0,0.0]
["Control_1 trimmed RNA-Seq report","Both",59.52,40.48,0.0,0.0]
["Control_3 trimmed RNA-Seq report","Both",51.34,48.66,0.0,0.0]
["Control_2 trimmed RNA-Seq report","Both",51.78,48.22,0.0,0.0]
["DRBD2_1 trimmed-1 RNA-Seq report","Both",79.03,20.97,0.0,0.0]
[]
["Strand specific setting: >90% of reads were mapped in the same orientation. Consider re-running the tool with a strand specific setting (\"Forward\"/\"Reverse\")."]
[]
["Distribution of biotypes"]
["Sample name","unknown"]
["DRBD2_2 trimmed RNA-Seq report-1",100.0]
["DRBD2_3 trimmed RNA-Seq report",100.0]
["DRBD2_4 trimmed RNA-Seq report",100.0]
["Control_1 trimmed RNA-Seq report",100.0]
["Control_3 trimmed RNA-Seq report",100.0]
["Control_2 trimmed RNA-Seq report",100.0]
["DRBD2_1 trimmed-1 RNA-Seq report",100.0]
[]
["Transcript length coverage"]
["Sample name","Difference between average 3’ and 5’ normalized counts","% reads mapping to transcripts that are longer than 10,000 bp"]
["DRBD2_2 trimmed RNA-Seq report-1",-8.02,1.36]
["DRBD2_3 trimmed RNA-Seq report",2.53,3.37]
["DRBD2_4 trimmed RNA-Seq report",3.64,3.27]
["Control_1 trimmed RNA-Seq report",5.45,1.75]
["Control_3 trimmed RNA-Seq report",-2.56,0.64]
["Control_2 trimmed RNA-Seq report",-7.94,1.88]
["DRBD2_1 trimmed-1 RNA-Seq report",2.96,1.58]