LOCUS Exported 16276 bp DNA circular 22-NOV-2018 FEATURES Location/Qualifiers gene 2029..2589 /label=Csy4 /note="Csy4" misc_feature 2599..2655 /label=P2A /note="P2A" polyA_signal 6801..6851 /note="polyA" misc_feature 6873..6878 /note="BsaI" misc_feature 7511..7586 /note="sgRNA" primer_bind 7608..7632 /note="TX056-C" /note="TX056-C;gaggaaggttTGAGGATCCatatgaagatgaagatgaaa" primer_bind complement(7608..7618) /note="TX056-B" /note="TX056-B;tcatatGGATCCTCAaaccttcctcttcttcttagga" misc_feature 7608..7612 /note="BamHI" terminator 7613..7862 /note="HSP terminater" /note="HSP terminater;color: #ffffff" primer_bind complement(7778..7801) /label=ZY010-R3 /note="ZY010-R3" primer_bind complement(7843..7862) /label=TC021R2 /note="TC021R2" misc_feature 7883..7907 /note="RB T-DNA repeat" /note="RB T-DNA repeat;right border repeat from nopaline C58 T-DNA;color: #ffe4c4" primer_bind complement(7911..7933) /note="ZY065-RB" CDS 9207..9836 /dnas_title="pVS1 StaA;color: #993366" /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /note="stability protein from Pseudomonas plasmid pVS1" /note="pVS1 StaA;color: #993366" /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 10270..11337 /dnas_title="pVS1 RepA;color: #993366" /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /note="replication protein from Pseudomonas plasmid pVS1" /note="pVS1 RepA;color: #993366" /protein_id="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQ AAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDK PGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSE GEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRF LYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQ AGPILVMRYRNLIEGEASAGS" /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQ AAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDK PGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSE GEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRF LYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQ AGPILVMRYRNLIEGEASAGS" misc_feature 11941..12081 /note="bom" /note="bom;basis of mobility region from pBR322;color: #a6acb3" primer_bind complement(12148..12167) /label=VR /note="VR" rep_origin complement(12267..12855) /direction=LEFT /note="ori" /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication;color: #ffff00" CDS complement(12942..13736) /dnas_title="KanR;confers resistance to kanamycin;color: #ccffcc" /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /note="aphA-3" /note="KanR;confers resistance to kanamycin;color: #ccffcc" /protein_id="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 14161..14185 /note="LB T-DNA repeat" /note="LB T-DNA repeat;left border repeat from nopaline C58 T-DNA;color: #ffe4c4" primer_bind 14164..14186 /label=ZY065-LB /note="ZY065-LB" polyA_signal 14263..14437 /note="CaMV poly" /note="CaMV poly(A) signal;cauliflower mosaic virus polyadenylation signal;color: #a6acb3" CDS complement(14463..15488) /dnas_title="hygromycin phosphotransferase" /codon_start=1 /product="hygromycin phosphotransferase" /note="hygromycin phosphotransferase" /note="Hyg;color: #993366" /db_xref="GI:53829093" /protein_id="AAU94554.1" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPGSWR" promoter complement(15527..16204) /note="CaMV 35S promoter" /note="CaMV 35S promoter (enhanced);cauliflower mosaic virus 35S promoter with a duplicated enhancer region;color: #ffffff; direction: LEFT" primer_bind complement(15596..15614) /label=ZY010-F /note="ZY010-F" primer_bind complement(287..307) /label=fJG53_R promoter 9..2004 /note="ZmUbi1 promoter" primer_bind 676..695 /label=fJG53_F primer complement(930..969) /note="ZY226-H" primer_bind 1040..1058 /note="TX067-ZmUbi-1" primer_bind complement(1165..1186) /note="TX067-ZmUbi-2" primer_bind 1885..1904 /label=fJG54_F primer 1591..1643 /note="ZY226-I" primer_bind 1891..1913 /note="TX067-ZmUbi-F" CDS 2656..6795 /dnas_title="Cas9-NLS" /note="Cas9" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSRADPKKKRK V" primer_bind 2656..2679 /note="ZY295-Cas9-HP-1" /note="" primer_bind complement(3333..3355) /note="ZY295-Cas9-HP-2" /note="" primer_bind complement(3824..3847) /note="ZY295-Cas9-HP-3" /note="" primer_bind 3730..3751 /note="ZY295-Cas9-HP-5" /note="" primer_bind complement(4888..4910) /note="ZY295-Cas9-HP-6" /note="" primer_bind 4782..4803 /note="ZY295-Cas9-HP-7" /note="" primer_bind 5886..5907 /note="ZY295-Cas9-HP-8" /note="" primer_bind complement(5976..6000) /note="ZY295-Cas9-HP-9" /note="" primer_bind 6327..6345 /note="TX056-A" misc_feature 7504..7509 /note="BsaI" gene complement(6879..7503) /label=ccdb /note="ccdb" misc_feature complement(7484..7503) /label=TC002R /note="TC002R" /note="Geneious type: primer_bind_reverse" BASE COUNT 4176 a 4026 c 4038 g 4036 t ORIGIN 1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct ctTgagataa tgagcattgc 61 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt 121 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact 181 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga 241 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct 301 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc 361 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt 421 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat 481 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa 541 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg 601 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg 661 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca 721 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag 781 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt 841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac 901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gaActccccc 961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct 1021 ctctaccttc tcAagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg 1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctActagcgt tcgtacacgg 1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga 1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc 1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg 1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg 1381 gtcgttcAag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt 1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga 1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga 1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttcA 1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat 1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga 1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc 1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat 1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt 1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc 1981 tgttgtttgg tgttacttCT GCAGcctgca ggtagatcgc tcgtcgacat ggatcattat 2041 cttgatatta gacttagacc tgatccagaa tttccaccag ctcaacttat gtctgttctt 2101 tttggaaaac ttcatcaagc tcttgttgct caaggaggag atagaattgg agtttctttt 2161 cctgatcttg atgaatcaag atcaagactt ggagaaagac ttagaattca tgcttctgct 2221 gatgatctta gagctttgct tgctagacct tggcttgaag gacttagaga tcatcttcaa 2281 tttggagaac cagctgttgt tccacatcca actccttata gacaagtttc aagagttcaa 2341 gctaaatcta atccagaaag acttagaaga agacttatga gaagacatga tctttctgaa 2401 gaagaagcta gaaaaagaat tcctgatact gttgctagag ctttggattt gccttttgtt 2461 acacttagat cacaatctac tggacaacat tttagacttt ttattagaca tggaccactt 2521 caagttactg ctgaagaagg aggatttact tgttatggac tttctaaggg aggttttgtt 2581 ccttggtttg gatctggagc tactaatttt tctcttctta agcaagctgg agatgttgaa 2641 gaaaatcctg gacccatgga taagaagtac tctatcggac tcgatatcgg aactaactct 2701 gtgggatggg ctgtgatcac cgatgagtac aaggtgccat ctaagaagtt caaggttctc 2761 ggaaacaccg ataggcactc tatcaagaaa aaccttatcg gtgctctcct cttcgattct 2821 ggtgaaactg ctgaggctac cagactcaag agaaccgcta gaagaaggta caccagaaga 2881 aagaacagga tctgctacct ccaagagatc ttctctaacg agatggctaa agtggatgat 2941 tcattcttcc acaggctcga agagtcattc ctcgtggaag aagataagaa gcacgagagg 3001 caccctatct tcggaaacat cgttgatgag gtggcatacc acgagaagta ccctactatc 3061 taccacctca gaaagaagct cgttgattct actgataagg ctgatctcag gctcatctac 3121 ctcgctctcg ctcacatgat caagttcaga ggacacttcc tcatcgaggg tgatctcaac 3181 cctgataact ctgatgtgga taagttgttc atccagctcg tgcagaccta caaccagctt 3241 ttcgaagaga accctatcaa cgcttcaggt gtggatgcta aggctatcct ctctgctagg 3301 ctctctaagt caagaaggct tgagaacctc attgctcagc tccctggtga gaagaagaac 3361 ggacttttcg gaaacttgat cgctctctct ctcggactca cccctaactt caagtctaac 3421 ttcgatctcg ctgaggatgc aaagctccag ctctcaaagg atacctacga tgatgatctc 3481 gataacctcc tcgctcagat cggagatcag tacgctgatt tgttcctcgc tgctaagaac 3541 ctctctgatg ctatcctcct cagtgatatc ctcagagtga acaccgagat caccaaggct 3601 ccactctcag cttctatgat caagagatac gatgagcacc accaggatct cacacttctc 3661 aaggctcttg ttagacagca gctcccagag aagtacaaag agattttctt cgatcagtct 3721 aagaacggat acgctggtta catcgatggt ggtgcatctc aagaagagtt ctacaagttc 3781 atcaagccta tcctcgagaa gatggatgga accgaggaac tcctcgtgaa gctcaataga 3841 gaggatcttc tcagaaagca gaggaccttc gataacggat ctatccctca tcagatccac 3901 ctcggagagt tgcacgctat ccttagaagg caagaggatt tctacccatt cctcaaggat 3961 aacagggaaa agattgagaa gattctcacc ttcagaatcc cttactacgt gggacctctc 4021 gctagaggaa actcaagatt cgcttggatg accagaaagt ctgaggaaac catcacccct 4081 tggaacttcg aagaggtggt ggataagggt gctagtgctc agtctttcat cgagaggatg 4141 accaacttcg ataagaacct tccaaacgag aaggtgctcc ctaagcactc tttgctctac 4201 gagtacttca ccgtgtacaa cgagttgacc aaggttaagt acgtgaccga gggaatgagg 4261 aagcctgctt ttttgtcagg tgagcaaaag aaggctatcg ttgatctctt gttcaagacc 4321 aacagaaagg tgaccgtgaa gcagctcaaa gaggattact tcaagaaaat cgagtgcttc 4381 gattcagttg agatttctgg tgttgaggat aggttcaacg catctctcgg aacctaccac 4441 gatctcctca agatcattaa ggataaggat ttcttggata acgaggaaaa cgaggatatc 4501 ttggaggata tcgttcttac cctcaccctc tttgaagata gagagatgat tgaagaaagg 4561 ctcaagacct acgctcatct cttcgatgat aaggtgatga agcagttgaa gagaagaaga 4621 tacactggtt ggggaaggct ctcaagaaag ctcattaacg gaatcaggga taagcagtct 4681 ggaaagacaa tccttgattt cctcaagtct gatggattcg ctaacagaaa cttcatgcag 4741 ctcatccacg atgattctct cacctttaaa gaggatatcc agaaggctca ggtttcagga 4801 cagggtgata gtctccatga gcatatcgct aacctcgctg gatctcctgc aatcaagaag 4861 ggaatcctcc agactgtgaa ggttgtggat gagttggtga aggtgatggg aaggcataag 4921 cctgagaaca tcgtgatcga aatggctaga gagaaccaga ccactcagaa gggacagaag 4981 aactctaggg aaaggatgaa gaggatcgag gaaggtatca aagagcttgg atctcagatc 5041 ctcaaagagc accctgttga gaacactcag ctccagaatg agaagctcta cctctactac 5101 ctccagaacg gaagggatat gtatgtggat caagagttgg atatcaacag gctctctgat 5161 tacgatgttg atcatatcgt gccacagtca ttcttgaagg atgattctat cgataacaag 5221 gtgctcacca ggtctgataa gaacaggggt aagagtgata acgtgccaag tgaagaggtt 5281 gtgaagaaaa tgaagaacta ttggaggcag ctcctcaacg ctaagctcat cactcagaga 5341 aagttcgata acttgactaa ggctgagagg ggaggactct ctgaattgga taaggcagga 5401 ttcatcaaga ggcagcttgt ggaaaccagg cagatcacta agcacgttgc acagatcctc 5461 gattctagga tgaacaccaa gtacgatgag aacgataagt tgatcaggga agtgaaggtt 5521 atcaccctca agtcaaagct cgtgtctgat ttcagaaagg atttccaatt ctacaaggtg 5581 agggaaatca acaactacca ccacgctcac gatgcttacc ttaacgctgt tgttggaacc 5641 gctctcatca agaagtatcc taagctcgag tcagagttcg tgtacggtga ttacaaggtg 5701 tacgatgtga ggaagatgat cgctaagtct gagcaagaga tcggaaaggc taccgctaag 5761 tatttcttct actctaacat catgaatttc ttcaagaccg agattaccct cgctaacggt 5821 gagatcagaa agaggccact catcgagaca aacggtgaaa caggtgagat cgtgtgggat 5881 aagggaaggg atttcgctac cgttagaaag gtgctctcta tgccacaggt gaacatcgtt 5941 aagaaaaccg aggtgcagac cggtggattc tctaaagagt ctatcctccc taagaggaac 6001 tctgataagc tcattgctag gaagaaggat tgggacccta agaaatacgg tggtttcgat 6061 tctcctaccg tggcttactc tgttctcgtt gtggctaagg ttgagaaggg aaagagtaag 6121 aagctcaagt ctgttaagga acttctcgga atcactatca tggaaaggtc atctttcgag 6181 aagaacccaa tcgatttcct cgaggctaag ggatacaaag aggttaagaa ggatctcatc 6241 atcaagctcc caaagtactc actcttcgaa ctcgagaacg gtagaaagag gatgctcgct 6301 tctgctggtg agcttcaaaa gggaaacgag cttgctctcc catctaagta cgttaacttt 6361 ctttacctcg cttctcacta cgagaagttg aagggatctc cagaagataa cgagcagaag 6421 caacttttcg ttgagcagca caagcactac ttggatgaga tcatcgagca gatctctgag 6481 ttctctaaaa gggtgatcct cgctgatgca aacctcgata aggtgttgtc tgcttacaac 6541 aagcacagag ataagcctat cagggaacag gcagagaaca tcatccatct cttcaccctt 6601 accaacctcg gtgctcctgc tgctttcaag tacttcgata caaccatcga taggaagaga 6661 tacacctcta ccaaagaagt gctcgatgct accctcatcc atcagtctat cactggactc 6721 tacgagacta ggatcgatct ctcacagctc ggtggtgatt caagggctga tcctaagaag 6781 aagaggaagg ttTGAggatc taaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 6841 aaaaaaaaaa agttcactgc cgtataggca gggagacctt atattcccca gaacatcagg 6901 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata 6961 acggaaaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata 7021 tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg 7081 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga 7141 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt 7201 ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg 7261 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct 7321 taccagaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca 7381 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca 7441 tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg 7501 cttggtctca gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac 7561 ttgaaaaagt ggcaccgagt cggtgcgttc actgccgtat aggcagggat ccatatgaag 7621 atgaagatga aatatttggt gtgtcaaata aaaagcttgt gtgcttaagt ttgtgttttt 7681 ttcttggctt gttgtgttat gaatttgtgg ctttttctaa tattaaatga atgtaagatc 7741 acattataat gaataaacaa atgtttctat aatccattgt gaatgttttg ttggatctct 7801 tctgcagcat ataactactg tatgtgctat ggtatggact atggaatatg attaaagata 7861 aggagctcaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga 7921 gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt 7981 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct 8041 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat 8101 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct 8161 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc 8221 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca 8281 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga 8341 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg 8401 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct 8461 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc 8521 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc 8581 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc 8641 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg 8701 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca 8761 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag 8821 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga 8881 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt 8941 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc 9001 gcgcacggct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg 9061 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga 9121 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta 9181 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg 9241 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg 9301 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg 9361 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg 9421 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg 9481 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca 9541 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa 9601 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg 9661 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg 9721 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg 9781 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta 9841 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc 9901 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt 9961 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg 10021 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa 10081 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac 10141 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg 10201 taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat 10261 cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac 10321 ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca 10381 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg 10441 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt 10501 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc 10561 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca 10621 gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac 10681 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag 10741 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg 10801 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc 10861 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc 10921 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag 10981 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg 11041 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc 11101 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc 11161 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg 11221 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg 11281 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt 11341 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggcctcttt 11401 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac 11461 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa 11521 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc 11581 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct 11641 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct 11701 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg 11761 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga 11821 tgacggtgaa aacctctgac acatgcagct cccggaaacg gtcacagctt gtctgtaagc 11881 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 11941 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca 12001 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta 12061 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 12121 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 12181 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 12241 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 12301 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 12361 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 12421 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 12481 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 12541 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 12601 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 12661 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 12721 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 12781 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 12841 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 12901 gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt 12961 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc 13021 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca 13081 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct 13141 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc 13201 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc 13261 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag 13321 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg 13381 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc 13441 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca 13501 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc 13561 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt 13621 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg 13681 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat 13741 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga 13801 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt 13861 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc 13921 ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga 13981 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat 14041 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct 14101 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg 14161 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg 14221 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact 14281 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac 14341 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc 14401 cttatctggg aactactcac acattattat ggagaaaata gagcttgtcg atcgttaatt 14461 aatcacctcc aagatcccgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact 14521 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca 14581 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg 14641 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc 14701 cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc 14761 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc 14821 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca 14881 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa 14941 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt 15001 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat 15061 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca 15121 gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc 15181 aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg 15241 aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa 15301 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc 15361 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg 15421 gacacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc 15481 tttttcatcc taggagagat agatttgtag agagagactg gtgatttcag cgtgtcctct 15541 ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt gggattgtgc 15601 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg 15661 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc 15721 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc 15781 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag 15841 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac 15901 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tatcacatca 15961 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg 16021 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt 16081 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg 16141 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt 16201 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt 16261 gtcgtgctcc accatg //