LOCUS Exported 15191 bp DNA circular 22-NOV-2018 FEATURES Location/Qualifiers CDS 2013..5795 /note="NLS-LbCpf1-NLS" misc_feature 2491..2514 /note=" /label=%22LbCpf1-seq1" primer_bind 3120..3143 /note=" /label=%22LbCpf1-seq2" primer_bind 3693..3712 /note=" /label=%22LbCpf1-seq3" primer_bind 4326..4349 /note=" /label=%22LbCpf1-seq4" primer_bind 4961..4982 /note=" /label=%22LbCpf1-seq5" primer_bind 5153..5176 /note="TX222-F" misc_feature 5664..5669 /note="BpiI" primer_bind 5842..5872 /note="TX231-F" gene complement(5853..6477) /note="ccdB" primer_bind complement(6456..6484) /note="TX231-R" primer_bind complement(6518..6532) /note="TX222-R" terminator 6527..6776 /note="Hsp terminator" misc_feature 6777..6782 /note="SacI" misc_feature 6797..6821 /note="RB T-DNA repeat" /note="right border repeat from nopaline C58 T-DNA" primer_bind complement(6825..6847) /note="ZY065-RB" CDS 8122..8751 /dnas_title="pVS1 StaA" /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /note="pVS1 StaA" /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 9185..10252 /dnas_title="pVS1 RepA" /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /note="pVS1 RepA" /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQ AAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDK PGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSE GEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRF LYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQ AGPILVMRYRNLIEGEASAGS" /note="" misc_feature 10856..10996 /note="bom" /note="basis of mobility region from pBR322" rep_origin complement(11182..11770) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(11857..12651) /dnas_title="KanR" /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 13076..13100 /note="LB T-DNA repeat" /note="left border repeat from nopaline C58 T-DNA" polyA_signal 13178..13352 /note="CaMV poly(A) signal" /note="cauliflower mosaic virus polyadenylation signal" CDS complement(13378..14403) /dnas_title="Hyg" /codon_start=1 /product="hygromycin phosphotransferase" /note="Hyg" /db_xref="GI:53829093" /protein_id="AAU94554.1" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPGSWR" promoter complement(14442..15119) /note="CaMV 35S promoter (enhanced)" /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" primer_bind complement(287..307) /label=fJG53_R promoter 9..2004 /note="ZmUbi1 promoter" primer_bind 676..695 /label=fJG53_F primer complement(930..969) /note="ZY226-H" primer_bind 1040..1058 /note="TX067-ZmUbi-1" primer_bind complement(1165..1186) /note="TX067-ZmUbi-2" primer_bind 1885..1904 /label=fJG54_F primer 1591..1643 /note="ZY226-I" primer_bind 1891..1913 /note="TX067-ZmUbi-F" polyA_signal 5795..5844 /note="polyA" repeat_region 6485..6504 /note="Lb 20nt DR" BASE COUNT 3946 a 3723 c 3815 g 3707 t ORIGIN 1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct ctTgagataa tgagcattgc 61 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt 121 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact 181 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga 241 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct 301 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc 361 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt 421 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat 481 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa 541 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg 601 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg 661 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca 721 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag 781 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt 841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac 901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gaActccccc 961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct 1021 ctctaccttc tcAagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg 1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctActagcgt tcgtacacgg 1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga 1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc 1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg 1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg 1381 gtcgttcAag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt 1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga 1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga 1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttcA 1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat 1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga 1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc 1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat 1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt 1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc 1981 tgttgtttgg tgttacttCT GCAGcctgca ggatggctcc taagaagaag cggaaggttg 2041 gtattcacgg ggtgcctgcg gcttcaaagc tcgagaaatt caccaactgt tattcgttga 2101 gcaaaacact gcggtttaaa gcgattccag tcggcaagac tcaagagaat atagacaata 2161 agcggctgtt ggtggaagat gaaaagcgcg cggaagacta caaaggggtg aagaagttgt 2221 tggacagata ctacctctct tttatcaatg atgtcttgca ctcaatcaaa ttgaagaatc 2281 tgaacaacta catctccctc ttcagaaaga aaacaaggac agaaaaggag aataaggaac 2341 ttgaaaattt ggagatcaat ctgaggaaag agatcgcgaa agcctttaaa ggcaacgaag 2401 gatacaaaag tctgttcaag aaggatataa ttgagacaat tttgccagag ttcctcgatg 2461 acaaggacga gattgcgctg gtcaattcgt tcaacggatt cacaacagca ttcacaggct 2521 tctttgataa tcgggaaaat atgttctctg aggaggcaaa gtccacttct attgcgttca 2581 ggtgtatcaa tgagaatctc actaggtaca tttccaacat ggatatcttt gagaaggttg 2641 acgcaatttt tgacaagcac gaagttcagg agattaagga gaagatcctc aattccgatt 2701 atgacgttga ggacttcttc gaaggtgagt tttttaattt cgtgctcact caagagggta 2761 tcgacgtgta taatgcgatc atcggtgggt tcgtgactga gtccggtgaa aagattaagg 2821 gattgaacga gtatatcaac ctttacaacc aaaagacgaa acagaagctg ccaaagttca 2881 agcctcttta caaacaggtt ctttcagacc gcgagtcact ctcgttctat ggggagggct 2941 acacttcgga tgaggaagtc ctggaggtgt tcaggaatac tctcaataag aattcggaga 3001 ttttctcttc tataaaaaaa ctggaaaagt tgtttaagaa ttttgacgaa tactctagcg 3061 ccggcatatt tgtgaaaaac ggcccggcca tatcaacgat aagtaaagat atcttcggcg 3121 aatggaacgt gatcagagac aaatggaacg cggagtatga cgatattcac ctgaagaaga 3181 aggctgtcgt aacggagaag tacgaggatg atcgcaggaa aagcttcaaa aagatcggaa 3241 gtttcagcct ggaacagttg caggagtatg ctgacgccga tcttagcgtc gtcgagaagt 3301 tgaaggagat aatcatccaa aaggtcgatg agatatataa agtctatgga tcaagtgaaa 3361 aactgttcga cgccgacttc gttttggaga agtccctgaa gaagaacgac gctgttgttg 3421 ccattatgaa ggatctgctc gacagcgtga agagtttcga gaactatatt aaggcttttt 3481 tcggggaggg gaaggagact aacagagatg agtccttcta cggagacttc gtcctcgcgt 3541 acgatatact ccttaaggta gaccacatct acgacgcaat cagaaattac gtgacacaaa 3601 agccgtacag caaggacaag ttcaaactct acttccagaa cccccagttc atgggcggct 3661 gggacaagga caaggaaacg gattacaggg ctacgatcct gaggtatggt tcaaaatact 3721 acttggcgat tatggacaag aagtacgcca agtgtctcca gaagattgac aaagacgatg 3781 tcaatggcaa ttatgagaag atcaactaca agctgcttcc gggtccgaac aagatgctcc 3841 caaaggtttt cttcagcaag aaatggatgg cctactataa cccaagcgag gacatccaga 3901 agatttataa gaacggtacg ttcaagaagg gcgacatgtt caatcttaac gactgtcaca 3961 agctgatcga cttcttcaaa gactcaatta gccggtaccc aaagtggtct aacgcctatg 4021 acttcaactt ttcggaaacc gagaagtaca aggatatagc cggattttat agagaggtgg 4081 aagagcaggg ctacaaggtg tcattcgagt ccgccagcaa gaaggaagtg gacaagctcg 4141 tggaagaggg taagctctac atgttccaga tttataataa agactttagc gataagagcc 4201 acgggacacc taatctccac acaatgtatt tcaagctgct cttcgacgag aataaccacg 4261 gccaaatcag gttgtcagga ggggctgaac tcttcatgcg gcgcgcatcc ctgaagaagg 4321 aggagcttgt agtccaccct gcgaatagtc caattgcgaa taagaacccg gacaatccta 4381 aaaagactac aacattgagc tacgacgtgt acaaggataa gaggttttcc gaggatcagt 4441 acgagctcca catcccgatt gcgatcaaca agtgcccaaa gaatattttc aagataaaca 4501 cagaggtgcg tgtactcctg aagcatgacg acaatcctta cgtcattggg attgatcggg 4561 gcgagaggaa cctcctctat attgtggtgg tggacgggaa ggggaacata gtcgaacagt 4621 actcccttaa cgaaataatt aacaatttca acggcatccg tatcaagacc gactaccatt 4681 cgttgctgga caagaaggag aaggagagat ttgaggcgcg gcaaaattgg acaagtatcg 4741 agaacatcaa ggaactcaaa gcaggttata tctctcaagt tgtgcataag atatgcgagc 4801 tggttgagaa gtatgacgca gtgatcgctc ttgaggacct caactcgggc tttaagaata 4861 gccgcgttaa agtggagaag caggtctatc aaaagttcga gaagatgctt atagataagc 4921 tcaactacat ggtcgataag aaatcgaacc catgtgccac cggcggcgca ctcaaaggtt 4981 accaaataac aaacaaattc gagtccttca aatcgatgag tactcagaat gggttcatat 5041 tttatatacc ggcgtggctt acgtctaaga tcgacccgtc aactggtttt gtcaacctgt 5101 tgaagacgaa atacacgtcc attgccgatt cgaaaaagtt catatctagt tttgatcgta 5161 ttatgtacgt cccagaggaa gatcttttcg agtttgctct cgactacaaa aacttttcgc 5221 ggaccgatgc ggattacatt aaaaaatgga aactctattc gtacggcaac agaatcagga 5281 tttttcgcaa ccctaagaag aataacgtct ttgattggga ggaagtttgc ttgactagcg 5341 cgtacaagga gctctttaat aagtatggca ttaactacca acagggtgat atcagagcac 5401 tgctttgcga acaatctgac aaggctttct actcatcctt catggctttg atgagcctga 5461 tgctccagat gagaaattca attacaggca gaaccgacgt ggatttcttg atctccccgg 5521 ttaaaaattc tgatggcatc ttttacgata gcaggaacta tgaagcgcaa gagaatgcga 5581 ttctgccaaa aaatgcagac gccaacggtg cctataacat cgccaggaaa gtcctgtggg 5641 cgatcggcca gttcaaaaag gccgaagacg aaaaattgga caaggtcaaa atcgctatca 5701 gcaacaaaga gtggctggag tatgctcaga catccgtaaa gcataagcgt cctgctgcca 5761 ccaaaaaggc cggacaggct aagaaaaaga agtgaaaaaa aaaaaaaaaa aaaaaaaaaa 5821 aaaaaaaaaa aaaaaaaaaa aaaaaggaga ccttatattc cccagaacat caggttaatg 5881 gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggaa 5941 accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc 6001 accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc cagggggatc 6061 accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa 6121 cggctctctc ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc 6181 agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc 6241 cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccag 6301 accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc 6361 actgtaatac gctgcttcat agcatacctc tttttgacat acttcgggta tacatatcag 6421 tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat ctggcttggt 6481 ctcgaatttc tactaagtgt agatggtacc cggccggaat tctagaatat gaagatgaag 6541 atgaaatatt tggtgtgtca aataaaaagc ttgtgtgctt aagtttgtgt ttttttcttg 6601 gcttgttgtg ttatgaattt gtggcttttt ctaatattaa atgaatgtaa gatcacatta 6661 taatgaataa acaaatgttt ctataatcca ttgtgaatgt tttgttggat ctcttctgca 6721 gcatataact actgtatgtg ctatggtatg gactatggaa tatgattaaa gataaggagc 6781 tcaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt 6841 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat 6901 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct 6961 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc 7021 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg 7081 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga 7141 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact 7201 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga 7261 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg 7321 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg 7381 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg 7441 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt 7501 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag 7561 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc 7621 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct 7681 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc 7741 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg 7801 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat 7861 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca 7921 cggctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc 7981 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt 8041 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa 8101 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca 8161 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt 8221 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat 8281 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc 8341 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc 8401 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg 8461 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta 8521 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc 8581 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc 8641 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct 8701 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag 8761 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca 8821 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact 8881 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga 8941 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa 9001 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca 9061 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc 9121 ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg 9181 tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt 9241 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc 9301 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc 9361 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt 9421 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt 9481 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg 9541 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt 9601 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga 9661 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc 9721 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca 9781 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg 9841 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat 9901 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt 9961 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg 10021 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga 10081 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg 10141 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct 10201 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga 10261 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggcc tctttcctgt 10321 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg 10381 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa 10441 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct 10501 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct 10561 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg 10621 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc 10681 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg 10741 gtgaaaacct ctgacacatg cagctcccgg aaacggtcac agcttgtctg taagcggatg 10801 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 10861 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 10921 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 10981 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 11041 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 11101 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 11161 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 11221 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 11281 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 11341 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 11401 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 11461 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 11521 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 11581 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 11641 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 11701 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 11761 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 11821 ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat 11881 ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac 11941 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca 12001 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac 12061 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc 12121 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt 12181 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct 12241 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca 12301 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca 12361 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg 12421 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc 12481 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt 12541 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg 12601 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt 12661 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac 12721 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag 12781 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga 12841 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc 12901 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca 12961 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg 13021 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca 13081 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt 13141 ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt 13201 ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact 13261 aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat 13321 ctgggaacta ctcacacatt attatggaga aaatagagct tgtcgatcgt taattaatca 13381 cctccaagat cccggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac 13441 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc 13501 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt 13561 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag 13621 ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat 13681 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa 13741 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt 13801 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat 13861 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca 13921 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc 13981 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat 14041 cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc 14101 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaattc 14161 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata 14221 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc 14281 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggacac 14341 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt 14401 catcctagga gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa 14461 tgaaatgaac ttccttatat agaggaaggt cttgcgaagg atagtgggat tgtgcgtcat 14521 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt 14581 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag 14641 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt 14701 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt 14761 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat 14821 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttatca catcaatcca 14881 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg 14941 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc 15001 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga 15061 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa 15121 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt 15181 gctccaccat g //