{"text_format":"2","workbook":"xlsx","n_sheets":3,"sheets":["Feuil1","Feuil2","Feuil3"],"not_rendered":{"charts":0,"images":0,"macros":0,"external_links":0,"defined_names":0,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"run_cost":"one","rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900"}

{"sheet":"Feuil1","index":0,"n_rows":52,"n_cols":3,"rows_kept":52,"cut":false,"hidden":false,"merged":[[1,0,1,2],[33,0,33,2],[41,0,41,2]]}
["Primer  ","Sequence (5’ to 3’)","Description"]
["Gene inactivation using group II intron (pQint)"]
["EBSuniv","CGAAATTAGAAACTTGCGTTCAGTAAAC","Universal primer for retargeting intron in pQint."]
["2241IBS","AAAACATATGATAATTATCCTTAGAAAGCAAGTTAGTGCGCCCAGATAGGGTG","Retarget intron to make antisense insertion in cphy2241 164 bp from start codon. Targeton score=10, e-value=0.021."]
["2241EBS1d","CAGATTGTACAAATGTGGTGATAACAGATAAGTCAAGTTACTTAACTTACCTTTCTTTGT","Retarget intron to make antisense insertion in cphy2241 164 bp from start codon. Targeton score=10, e-value=0.021."]
["2241EBS2","TGAACGCAAGTTTCTAATTTCGATTCTTTCTCGATAGAGGAAAGTGTCT","Retarget intron to make antisense insertion in cphy2241 164 bp from start codon. Targeton score=10, e-value=0.021."]
["OK2241F","CCAACCGACAATTCCACCAA","Primers flanking cphy2241 to confirm intron insertion."]
["OK2241R","ACTTGGGGTTTGTGACAGGA","Primers flanking cphy2241 to confirm intron insertion."]
["2465IBS","AAAACATATGATAATTATCCTTAGCTAACAGGAGAGTGCGCCCAGATAGGGTG","Retarget intron to make antisense insertion in cphy2465 293 bp from start codon. Targeton score=8.47, e-value=0.077."]
["2465EBS1d","CAGATTGTACAAATGTGGTGATAACAGATAAGTCAGGAGAGATAACTTACCTTTCTTTGT","Retarget intron to make antisense insertion in cphy2465 293 bp from start codon. Targeton score=8.47, e-value=0.077."]
["2465EBS2","TGAACGCAAGTTTCTAATTTCGATTTTAGCTCGATAGAGGAAAGTGTCT","Retarget intron to make antisense insertion in cphy2465 293 bp from start codon. Targeton score=8.47, e-value=0.077."]
["OK2465F","CACCATCAACATAGGCTGCC","Primers flanking cphy2465 to confirm intron insertion."]
["OK2465R","GGGGATTGTCAAAGGTAGGC","Primers flanking cphy2465 to confirm intron insertion."]
["2732IBS","AAAACATATGATAATTATCCTTATTTGCCCTGCTTGTGCGCCCAGATAGGGTG","Retarget intron to make antisense insertion in cphy2732 622 bp from start codon. Clostron (Perutka)  score=5.24, e-value=0."]
["2732EBS1d","CAGATTGTACAAATGTGGTGATAACAGATAAGTCCTGCTTCGTAACTTACCTTTCTTTGT","Retarget intron to make antisense insertion in cphy2732 622 bp from start codon. Clostron (Perutka)  score=5.24, e-value=0."]
["2732EBS2","TGAACGCAAGTTTCTAATTTCGGTTGCAAATCGATAGAGGAAAGTGTCT","Retarget intron to make antisense insertion in cphy2732 622 bp from start codon. Clostron (Perutka)  score=5.24, e-value=0."]
["OK2732","TCGTCTACCCCTCCTATACGA","Primers flanking cphy2732 to confirm intron insertion."]
["OK2732","ACTGCATCTGTATTCCGCTT","Primers flanking cphy2732 to confirm intron insertion."]
["3589IBS","AAAACATATGATAATTATCCTTACATGGCAAGAAGGTGCGCCCAGATAGGGTG","Retarget intron to make antisense insertion in cphy3589 332 bp from start codon. Targeton score=8.43, e-value=0.079."]
["3589EBS1d","CAGATTGTACAAATGTGGTGATAACAGATAAGTCAAGAAGATTAACTTACCTTTCTTTGT","Retarget intron to make antisense insertion in cphy3589 332 bp from start codon. Targeton score=8.43, e-value=0.079."]
["3589EBS2","TGAACGCAAGTTTCTAATTTCGGTTCCATGTCGATAGAGGAAAGTGTCT","Retarget intron to make antisense insertion in cphy3589 332 bp from start codon. Targeton score=8.43, e-value=0.079."]
["OK3589F","AAGCGTTCCATTGGTTGACA","Primers flanking cphy3589 to confirm intron insertion."]
["OK3589R","TAGGAGGAGGTGCAGGTTTT","Primers flanking cphy3589 to confirm intron insertion."]
["3859IBS","AAAACATATGATAATTATCCTTATTGCACCATCGAGTGCGCCCAGATAGGGTG","Retarget intron to make antisense insertion in cphy3859 586 bp from start codon. Targeton score=8.01, e-value=0.108."]
["3859EBS1d","CAGATTGTACAAATGTGGTGATAACAGATAAGTCCATCGATTTAACTTACCTTTCTTTGT ","Retarget intron to make antisense insertion in cphy3859 586 bp from start codon. Targeton score=8.01, e-value=0.108."]
["3859EBS2","TGAACGCAAGTTTCTAATTTCGGTTTGCAATCGATAGAGGAAAGTGTCT","Retarget intron to make antisense insertion in cphy3859 586 bp from start codon. Targeton score=8.01, e-value=0.108."]
["OK3859F","TGTCTCTCATGGTGCGAAGT","Primers flanking cphy3859 to confirm intron insertion."]
["OK3859R","AGTTTTCATAGCAACCCTCCTC","Primers flanking cphy3859 to confirm intron insertion."]
["OKtargetF","CGCCAGAAAACAAAAGAAAGCC","Confirm retargeting of pQint by sequencing"]
["OktargetR","CGCTTTCGTTTCGTTCCCAT","Confirm retargeting of pQint by sequencing"]
["Intron_Right","TGGGAAATGGCAATGATAGCG","Amplify ligation product during the inverse PCR"]
["Intron_Left","AACTCATCGCTTTCCAACCG","Amplify ligation product during the inverse PCR"]
[]
["Expression of His-tagged hexose kinases"]
["2237_F ","AAAGAAGGAGATAGGATCATGATACATCCTAGTATAGAAATACTG","Amplify cphy2237 coding sequence. 5’ red sequence is for ligation-independent clonging in pET-22B(+)"]
["2237_R ","GTGTAATGGATAGTGATCTTAATGGTGATGGTGATGATGAAGTTTATCCAAATGAATTGCTCC","Amplify cphy2237 coding sequence. 5’ red sequence for ligation-independent clonging in pET-22B(+).\nGreen sequence is C-terminal His tag."]
["0329_F ","AAAGAAGGAGATAGGATCATGGACAAGTTTTGCTTTGGAATTG","Amplify cphy0329 coding sequence. 5’ red sequence is for ligation-independent clonging in pET-22B(+)"]
["0329_R ","GTGTAATGGATAGTGATCTTAATGGTGATGGTGATGATGGCCAATCACTAATTTTGCACAAC","Amplify cphy0329 coding sequence. 5’ red sequence for ligation-independent clonging in pET-22B(+).\nGreen sequence is C-terminal His tag."]
["P2116","ATCGAGATCTCGATCCCGCG","Confirm cloning in pET-22(B) by sequencing"]
["P2117","GCAGCAGCCAACTCAGCTTCC","Confirm cloning in pET-22(B) by sequencing"]
[]
["Expression of His-tagged extracellular solute binding proteins"]
["2243_pur_F","AAAGAAGGAGATAGGATCatgAAAGGATCTTCTTTAAATGTAGG","Amplify cphy2243 coding sequence. 5’ red sequence is for ligation-independent clonging in pET-22B(+)"]
["2243_pur_R","GTGTAATGGATAGTGATCTTAATGGTGATGGTGATGATGtTCACCGATATAAGCAGCAT","Amplify cphy2243 coding sequence. 5’ red sequence for ligation-independent clonging in pET-22B(+).\nGreen sequence is C-terminal His tag."]
["2466_pur_F","AAAGAAGGAGATAGGATCatgAAGAACGACCAAAAAGCAAACAAG","Amplify cphy2466 coding sequence. 5’ red sequence is for ligation-independent clonging in pET-22B(+)"]
["2466_pur_R","GTGTAATGGATAGTGATCTTAATGGTGATGGTGATGATGTTTACTAAGTTCTGGATGCTT","Amplify cphy2466 coding sequence. 5’ red sequence for ligation-independent clonging in pET-22B(+).\nGreen sequence is C-terminal His tag."]
["2733_pur_F","AAAGAAGGAGATAGGATCatgAGCAAGAACGATGGACCAAAG","Amplify cphy2733 coding sequence. 5’ red sequence is for ligation-independent clonging in pET-22B(+)"]
["2733_pur_R","GTGTAATGGATAGTGATCTTAATGGTGATGGTGATGATGGTTGTTTAAAGAATCTAAAATTTCC","Amplify cphy2733 coding sequence. 5’ red sequence for ligation-independent clonging in pET-22B(+).\nGreen sequence is C-terminal His tag."]
["3590_pur_F","AAAGAAGGAGATAGGATCatgAAGACAGGTAGTAACAATGCAGG","Amplify cphy3590 coding sequence. 5’ red sequence is for ligation-independent clonging in pET-22B(+)"]
["3590_pur_R","GTGTAATGGATAGTGATCTTAATGGTGATGGTGATGATGTTGACCCATCTGGAATTCTAA","Amplify cphy3590 coding sequence. 5’ red sequence for ligation-independent clonging in pET-22B(+).\nGreen sequence is C-terminal His tag."]
["3858_pur_F","AAAGAAGGAGATAGGATCatgGCCTGCACTAAGAAAGAGGATC","Amplify cphy3858 coding sequence. 5’ red sequence is for ligation-independent clonging in pET-22B(+)"]
["3858_pur_R","GTGTAATGGATAGTGATCTTAATGGTGATGGTGATGATGTTGAGCAGCTGTTAAATCTAAAAC","Amplify cphy3858 coding sequence. 5’ red sequence for ligation-independent clonging in pET-22B(+).\nGreen sequence is C-terminal His tag."]

{"sheet":"Feuil2","index":1,"n_rows":0,"n_cols":0,"rows_kept":0,"cut":false,"hidden":false,"merged":[]}

{"sheet":"Feuil3","index":2,"n_rows":0,"n_cols":0,"rows_kept":0,"cut":false,"hidden":false,"merged":[]}