Figure 2 Supplementary Table 1. Effect size and ANOVA statistics for optogenetics Fixed 12s delay, varied optogenetic perturbation, correct rate Effect size (Cohen's d) 3s Early 3s Mid 3s Late 6s Early 6s Mid 6s Late 10s Ctrl 0.36 0.42 0.68 0.48 0.54 0.02 0.52 ChR2 0.29 0.58 1.07 0.72 0.67 1.52 1.50 Mixed-between-within-ANOVA SumSq DF MeanSq F pValue __________ ___ __________ ______ __________ (Intercept) 1.1219e+06 1 1.1219e+06 11128 1.3863e-26 Genotype 165.68 1 165.68 1.6434 0.21613 Error 1814.7 18 100.82 (Intercept):OptoCondition 252.36 7 36.051 5.6494 1.0913e-05 Genotype:OptoCondition 171.99 7 24.57 3.8502 0.00079122 Error(OptoCondition) 804.06 126 6.3814 Post-hoc paired-permutation test of 10000 repeats, Bonferroni adjusted p-value 3s Early 3s Mid 3s Late 6s Early 6s Mid 6s Late 10s Ctrl 1.413 1.014 0.178 0.276 0.359 6.471 0.834 ChR2 1.569 0.751 0.046 0.025 0.130 0.026 0.029 Fixed 12s delay, varied optogenetic perturbation, false alarm rate Effect size (Cohen's d) 3s Early 3s Mid 3s Late 6s Early 6s Mid 6s Late 10s Ctrl -0.40 -0.49 -0.46 -0.67 -0.52 -0.19 -0.56 ChR2 -0.08 -0.38 -1.13 -0.56 -0.45 -1.52 -1.66 Mixed-between-within-ANOVA SumSq DF MeanSq F pValue ______ ___ ______ _______ __________ (Intercept) 36981 1 36981 134.42 8.7652e-10 Genotype 29.439 1 29.439 0.10701 0.74735 Error 4952.1 18 275.12 (Intercept):OptoCondition 627.56 7 89.651 7.0342 4.4305e-07 Genotype:OptoCondition 472.71 7 67.531 5.2986 2.4952e-05 Error(OptoCondition) 1605.9 126 12.745 Post-hoc paired-permutation test of 10000 repeats, Bonferroni adjusted p-value 3s Early 3s Mid 3s Late 6s Early 6s Mid 6s Late 10s Ctrl 0.699 0.603 0.337 0.068 0.311 2.806 0.223 ChR2 4.997 1.000 0.080 0.235 1.450 0.051 0.021 Fixed 12s delay, varied optogenetic perturbation, miss rate Effect size (Cohen's d) 3s Early 3s Mid 3s Late 6s Early 6s Mid 6s Late 10s Ctrl -0.03 0.02 -0.20 0.12 -0.10 0.14 0.00 ChR2 -0.24 -0.28 -0.08 -0.22 -0.32 -0.30 -0.55 Mixed-between-within-ANOVA SumSq DF MeanSq F pValue ______ ___ ______ _______ __________ (Intercept) 42602 1 42602 99.508 9.2762e-09 Genotype 412.81 1 412.81 0.96423 0.33914 Error 7706.2 18 428.12 (Intercept):OptoCondition 83.909 7 11.987 0.84111 0.55531 Genotype:OptoCondition 125.79 7 17.97 1.2609 0.27505 Error(OptoCondition) 1795.7 126 14.251 Post-hoc paired-permutation test of 10000 repeats, Bonferroni adjusted p-value 3s Early 3s Mid 3s Late 6s Early 6s Mid 6s Late 10s Ctrl 6.080 5.599 3.434 3.470 3.198 2.358 6.894 ChR2 2.003 1.611 5.245 1.947 0.890 1.168 0.118 Effect of laser duration and laser-offset-test-onset interval, 12s delay, correct rate Repeated-measure ANOVA SumSq DF MeanSq F pValue __________ __ __________ ______ __________ (Intercept) 4.307e+05 1 4.307e+05 4603.5 2.4782e-12 Error 748.48 8 93.56 (Intercept):Duration 3.53e+05 1 3.53e+05 4185.9 3.6229e-12 Error(Duration) 674.64 8 84.329 (Intercept):Interval 2.8852e+05 1 2.8852e+05 4685.5 2.3096e-12 Error(Interval) 492.62 8 61.577 (Intercept):Duration:Interval 3.2191e+05 1 3.2191e+05 4763.8 2.1616e-12 Error(Duration:Interval) 540.59 8 67.574 Effect of laser duration and laser-offset-test-onset interval, 12s delay, false alarm rate Repeated-measure ANOVA SumSq DF MeanSq F pValue ______ __ ______ ______ __________ (Intercept) 16788 1 16788 83.489 1.6578e-05 Error 1608.7 8 201.08 (Intercept):Duration 15402 1 15402 99.58 8.6227e-06 Error(Duration) 1237.4 8 154.67 (Intercept):Interval 8169.7 1 8169.7 60.212 5.4355e-05 Error(Interval) 1085.5 8 135.68 (Intercept):Duration:Interval 10133 1 10133 68.687 3.3831e-05 Error(Duration:Interval) 1180.2 8 147.53 Effect of laser duration and laser-offset-test-onset interval, 12s delay, miss rate Repeated-measure ANOVA SumSq DF MeanSq F pValue ______ __ ______ ______ __________ (Intercept) 21118 1 21118 48.545 0.00011637 Error 3480.2 8 435.02 (Intercept):Duration 18439 1 18439 48.454 0.00011713 Error(Duration) 3044.4 8 380.55 (Intercept):Interval 13417 1 13417 48.505 0.00011671 Error(Interval) 2212.9 8 276.61 (Intercept):Duration:Interval 15383 1 15383 49.959 0.00010526 Error(Duration:Interval) 2463.4 8 307.92 Fixed 20s delay, varied optogenetic perturbation, correct rate Effect size (Cohen's d) 3s Early 3s Mid 3s Late 6s Early 6s Mid 6s Late 12s Early 12s Mid 12s Late Ctrl 0.67 1.47 0.61 1.45 -0.41 -0.03 0.71 0.93 1.07 ChR2 0.43 1.34 2.01 0.92 1.42 1.41 0.87 0.95 1.83 Mixed-between-within-ANOVA SumSq DF MeanSq F pValue __________ ___ __________ _______ __________ (Intercept) 1.1831e+06 1 1.1831e+06 11462 8.4284e-22 Gene 99.128 1 99.128 0.96031 0.34374 Error 1445.1 14 103.22 (Intercept):OptoCondition 392.53 9 43.615 4.2871 7.1013e-05 Gene:OptoCondition 244.91 9 27.212 2.6748 0.0070858 Error(OptoCondition) 1281.8 126 10.173 Post-hoc paired-permutation test of 10000 repeats, Bonferroni adjusted p-value 3s Early 3s Mid 3s Late 6s Early 6s Mid 6s Late 12s Early 12s Mid 12s Late Ctrl 2.066 0.125 1.984 0.304 3.794 8.433 2.785 0.499 0.564 ChR2 1.800 0.061 0.025 0.292 0.097 0.075 0.254 0.443 0.034 Fixed 20s delay, varied optogenetic perturbation, false alarm rate Effect size (Cohen's d) 3s Early 3s Mid 3s Late 6s Early 6s Mid 6s Late 12s Early 12s Mid 12s Late Ctrl -0.65 -0.89 -0.74 -1.57 -0.36 -0.20 -0.96 -0.49 -1.08 ChR2 -0.55 -1.15 -1.80 -0.86 -1.06 -1.21 -0.63 -0.99 -1.50 Mixed-between-within-ANOVA SumSq DF MeanSq F pValue ______ ___ ______ ______ __________ (Intercept) 38498 1 38498 93.008 1.4607e-07 Gene 689.74 1 689.74 1.6664 0.21766 Error 5794.9 14 413.92 (Intercept):OptoCondition 989.89 9 109.99 4.2232 8.5281e-05 Gene:OptoCondition 521.37 9 57.93 2.2244 0.024474 Error(OptoCondition) 3281.5 126 26.043 Post-hoc paired-permutation test of 10000 repeats, Bonferroni adjusted p-value 3s Early 3s Mid 3s Late 6s Early 6s Mid 6s Late 12s Early 12s Mid 12s Late Ctrl 1.127 0.585 1.593 0.292 3.670 6.152 0.139 3.212 0.149 ChR2 0.599 0.060 0.039 0.280 0.000 0.104 0.616 0.246 0.034 Fixed 20s delay, varied optogenetic perturbation, miss rate Effect size (Cohen's d) 3s Early 3s Mid 3s Late 6s Early 6s Mid 6s Late 12s Early 12s Mid 12s Late Ctrl -0.16 -0.47 0.06 -0.75 0.75 0.25 -0.03 -0.43 0.07 ChR2 -0.12 -0.05 -0.47 -0.47 -0.38 -0.13 -0.58 -0.00 -0.39 Mixed-between-within-ANOVA SumSq DF MeanSq F pValue ______ ___ ______ _______ __________ (Intercept) 19132 1 19132 60.055 1.9792e-06 Gene 40.326 1 40.326 0.12658 0.72731 Error 4460.1 14 318.58 (Intercept):OptoCondition 211.08 9 23.454 1.2032 0.29882 Gene:OptoCondition 311.79 9 34.643 1.7772 0.078832 Error(OptoCondition) 2456.2 126 19.494 Post-hoc paired-permutation test of 10000 repeats, Bonferroni adjusted p-value 3s Early 3s Mid 3s Late 6s Early 6s Mid 6s Late 12s Early 12s Mid 12s Late Ctrl 6.341 2.509 8.012 0.132 1.912 3.383 8.708 0.820 7.621 ChR2 7.679 8.109 1.240 0.637 1.992 7.352 0.620 9.000 2.015 Effect of laser duration and laser-offset-test-onset interval, 20s delay, correct rate Repeated-measure ANOVA SumSq DF MeanSq F pValue __________ __ __________ ______ __________ (Intercept) 5.9059e+05 1 5.9059e+05 4388 3.0011e-12 Error 1076.7 8 134.59 (Intercept):Duration 4.5673e+05 1 4.5673e+05 3350.7 8.809e-12 Error(Duration) 1090.5 8 136.31 (Intercept):Interval 4.2852e+05 1 4.2852e+05 3978.6 4.4379e-12 Error(Interval) 861.66 8 107.71 (Intercept):Duration:Interval 3.2729e+05 1 3.2729e+05 2778.2 1.8606e-11 Error(Duration:Interval) 942.44 8 117.81 Effect of laser duration and laser-offset-test-onset interval, 20s delay, false alarm rate Repeated-measure ANOVA SumSq DF MeanSq F pValue ______ __ ______ ______ __________ (Intercept) 27743 1 27743 47.212 0.00012822 Error 4701.1 8 587.64 (Intercept):Duration 22379 1 22379 38.946 0.0002482 Error(Duration) 4596.9 8 574.61 (Intercept):Interval 16046 1 16046 44.851 0.00015317 Error(Interval) 2862.1 8 357.76 (Intercept):Duration:Interval 13111 1 13111 34.441 0.00037479 Error(Duration:Interval) 3045.5 8 380.69 Effect of laser duration and laser-offset-test-onset interval, 20s delay, miss rate Repeated-measure ANOVA SumSq DF MeanSq F pValue ______ __ ______ ______ _________ (Intercept) 9299.7 1 9299.7 20.615 0.0018983 Error 3609 8 451.12 (Intercept):Duration 7432.7 1 7432.7 21.001 0.0017956 Error(Duration) 2831.3 8 353.92 (Intercept):Interval 6540.7 1 6540.7 22.061 0.0015476 Error(Interval) 2371.8 8 296.48 (Intercept):Duration:Interval 5464 1 5464 21.373 0.0017033 Error(Duration:Interval) 2045.2 8 255.65 Fixed 3s late delay laser, varied delay duration, correct rate Effect size (Cohen's d) 5s Delay 8s Delay 12s Delay 20s Delay Ctrl -0.32 0.12 0.42 0.94 ChR2 1.06 1.21 1.92 2.26 Mixed-between-within-ANOVA SumSq DF MeanSq F pValue __________ __ __________ ______ __________ (Intercept) 1.0114e+06 1 1.0114e+06 20777 1.3154e-23 Gene 140.92 1 140.92 2.8949 0.11096 Error 681.52 14 48.68 (Intercept):Delay 7.9097e+05 1 7.9097e+05 21875 9.1732e-24 Gene:Delay 81.475 1 81.475 2.2533 0.15554 Error(Delay) 506.21 14 36.158 (Intercept):Laser 88.463 1 88.463 5.3516 0.036415 Gene:Laser 113.72 1 113.72 6.8798 0.020061 Error(Laser) 231.42 14 16.53 (Intercept):Delay:Laser 66.548 1 66.548 4.1651 0.060587 Gene:Delay:Laser 58.842 1 58.842 3.6828 0.075594 Error(Delay:Laser) 223.68 14 15.977 Post-hoc paired-permutation test of 10000 repeats, Bonferroni adjusted p-value 5s Delay 8s Delay 12s Delay 20s Delay Ctrl 0.223 0.878 0.747 0.856 ChR2 0.025 0.038 0.064 0.284 Fixed 3s late delay laser, varied delay duration, false alarm rate Effect size (Cohen's d) 5s Delay 8s Delay 12s Delay 20s Delay Ctrl 0.37 0.22 -0.34 0.23 ChR2 -0.38 -0.96 -0.80 -1.17 Mixed-between-within-ANOVA SumSq DF MeanSq F pValue ______ __ ______ ______ __________ (Intercept) 11312 1 11312 147.68 7.9536e-09 Gene 249.61 1 249.61 3.2587 0.092591 Error 1072.4 14 76.599 (Intercept):Delay 9169.2 1 9169.2 121.23 2.7998e-08 Gene:Delay 94.956 1 94.956 1.2555 0.28136 Error(Delay) 1058.9 14 75.634 (Intercept):Laser 69.545 1 69.545 4.1992 0.059666 Gene:Laser 245.86 1 245.86 14.845 0.0017573 Error(Laser) 231.86 14 16.561 (Intercept):Delay:Laser 136.6 1 136.6 6.9726 0.019383 Gene:Delay:Laser 182.15 1 182.15 9.2979 0.0086627 Error(Delay:Laser) 274.27 14 19.591 Post-hoc paired-permutation test of 10000 repeats, Bonferroni adjusted p-value 5s Delay 8s Delay 12s Delay 20s Delay Ctrl 0.258 0.062 0.937 0.528 ChR2 0.393 0.039 0.365 0.022 Fixed 3s late delay laser, varied delay duration, miss rate Effect size (Cohen's d) 5s Delay 8s Delay 12s Delay 20s Delay Ctrl 0.20 -0.30 -0.32 -1.35 ChR2 -0.68 -0.71 -0.99 -1.42 Mixed-between-within-ANOVA SumSq DF MeanSq F pValue ______ __ ______ _______ __________ (Intercept) 16191 1 16191 74.67 5.5198e-07 Gene 62.992 1 62.992 0.29051 0.59836 Error 3035.7 14 216.83 (Intercept):Delay 17741 1 17741 90.86 1.6857e-07 Gene:Delay 68.964 1 68.964 0.35319 0.5618 Error(Delay) 2733.7 14 195.26 (Intercept):Laser 109.72 1 109.72 1.8168 0.1991 Gene:Laser 31.868 1 31.868 0.52767 0.47956 Error(Laser) 845.52 14 60.395 (Intercept):Delay:Laser 21.44 1 21.44 0.44223 0.51686 Gene:Delay:Laser 3.3962 1 3.3962 0.07005 0.79512 Error(Delay:Laser) 678.76 14 48.483 Post-hoc paired-permutation test of 10000 repeats, Bonferroni adjusted p-value 5s Delay 8s Delay 12s Delay 20s Delay Ctrl 0.610 0.060 0.619 0.687 ChR2 0.083 0.148 0.092 0.191