This PDF file includes:

• Supplemental Materials and Methods
• Experimental Protocols
• Supplementary Note A. Development of the conditions for the dynamic reaction network by characterization of the individual enzyme reactions
• Supplementary Note B. Routine of GE analysis: From the agarose gel to an average chain length
• Supplementary Note C. ATP-fueled transient, dynamic steady-state DNA polymerization system
• Supplementary Note D. DySS and molecular exchange in ATP-fueled dissociative dynamic covalent DNA systems
• Table S1. Oligonucleotide sequences.
• Fig. S1. Hybridization of the self-complementary ends of the DNA monomer strands M₁ in dependence of temperature and ligation reaction catalyzed by T4 DNA ligase.
• Fig. S2. Ligation kinetics of the DNA chain growth as a function of T4 DNA ligase concentration.
• Fig. S3. Ligation kinetics of the DNA chain growth as a function of ATP concentration.
• Fig. S4. Time-dependent T4 DNA ligase catalyzed ligation reaction.
• Fig. S5. Restriction kinetics of the DNA chain cleavage as a function of BamHI concentration.
• Fig. S6. Routine for analysis of GE data: From the agarose GE to an average DNA chain length bp¯w.
• Fig. S7. Control of dispersity in the DySS DNA polymerization system.
• Fig. S8. Refueling experiments of the transient DySS DNA polymerization system.
• Fig. S9. Average chain length in the transient DySS DNA polymerization system in dependence of the concentration of the DNA monomer M₁.
• Fig. S10. Characterization of the FRET duplex F and its cleaved and religated DNA fragments as used for in situ modulation of the DySS.
• Fig. S11. ATP-dependent temporal control of the dynamic DNA bond with transient DySS FRET duplex formation.
• References (40, 41)

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