This PDF file includes:

• Fig. S1. Characterization of cell decision-making at trifurcating Ψ-like branch channels of different hydraulic resistances.
• Fig. S2. Effects of different cytoskeletal constituents on cell decision-making in response to hydraulic resistance.
• Fig. S3. Hydrostatic pressure induces intracellular calcium increase and a thicker cortical actin meshwork.
• Fig. S4. High hydraulic resistance results in larger changes of membrane curvature.
• Fig. S5. Variations in bleb size and cortical actin intensity reach a minimum at the decision-making time point, suggesting a physical balance between internal and external cell forces.
• Table S1. Dimensions of the different trifurcated designs.
• Table S2. Comparison between the experimental and theoretical probabilities of cells entering branches in two different devices.
• Table S3. Comparison between the experimental and theoretical probabilities of cells entering branches in multiple devices.

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