Quantification of 24 circulating endocannabinoids, endocannabinoid-related compounds, and their phospholipid precursors in human plasma by UHPLC-MS/MS

Supplemental Data

• Supplementary figure 1 (.pdf, 247 KB) - GC-MS chromatograms of synthesized fatty acid ethanolamides and deuterated standards.
• Supplementary figure 2 (.pdf, 66 KB) - Release of 2-AG and 1-AG during the incubation of plasma that was acidified to pH 4.7 and pH 3.8, respectively.
• Supplementary table 1 (.pdf, 86 KB) - Analytical range of endocannabinoids and ERCs used for calibration and validation experiments.
• Supplementary table 2 (.pdf, 85 KB) - Quantifier and qualifier mass transitions of all analytes investigated in this study.
• Supplementary table 3 (.pdf, 88 KB) - Stability of endogenous glycerol esters and representative fatty acid ethanolamides during storage at -80 ??C.
• Supplementary table 4 (.pdf, 91 KB) - Influence of matrix on electrospray ionization efficiency of isotope labelled standards in plasma and NAPE extracts, and total recovery of spiked IS from all tested matrices.
• Supplementary table 5 (.pdf, 101 KB) - Linearity of calibration curves in solvent and in spiked matrix.