This PDF file includes:

• Fig. S1. Zcwpw1 is generally expressed in different tissues.
• Fig. S2. The SC structure appeared normal in Zcwpw1^−/− spermatocytes.
• Fig. S3. Representative images of leptotene and zygotene stages in Zcwpw1^−/− spermatocytes.
• Fig. S4. The SC structure appeared normal in Zcwpw1^−/− oocytes.
• Fig. S5. Protein profiling analysis of PD14 wild-type and Zcwpw1^−/− testes.
• Table S1. List of 94 differentially expressed proteins in PD14 Zcwpw1^+/+ and Zcwpw1^−/− testes by HPLC-MS.

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