The PDF file includes:

• Fig. S1. Purification of CD4 T cells from young and old mice.
• Fig. S2. Dimensionality reduction of scRNA-seq data.
• Fig. S3. Subset identity and abundance in mice used for scRNA-seq.
• Fig. S4. RECs activation state and correlation with serum cytokines.
• Fig. S5. RECs abundance with time and across different immunological sites.
• Fig. S6. Gene regulatory networks and cytokine secretion in RECs.
• Fig. S7. Characterization of T helper polarizations within RECs.

Download PDF

Other Supplementary Material for this manuscript includes the following:

• Table S1 (Microsoft Excel format). The number of cells per mouse before and after quality control.
• Table S2 (Microsoft Excel format). Subset and age-group expression of genes.
• Table S3 (Microsoft Excel format). RECs comparison to closely related subsets.