This PDF file includes:

• Table S1. Sequence of the synthesized oligonucleotides.
• Fig. S1. Annealing and gene silencing ability of synthesized siRNA.
• Fig. S2. Chemical structure of DEX-MAES.
• Fig. S3. Schematic illustration of siRNA conjugation to hydrogels via Michael-addition reaction and its release.
• Fig. S4. Effect of hydrogel concentration on the tethered siRNA release behavior and bioactivity of released siRNA.
• Fig. S5. Lipofectamine-mediated bioactivity of released siRNA from siRNA-tethered hydrogels.
• Fig. S6. Transfection reagent-mediated bioactivity of siRNA loaded or tethered in the hydrogels.
• Fig. S7. Schematic illustration of siRNA conjugation to hydrogels via photoconjugation and its release.
• Fig. S8. Additional release profiles of tethered siGFP from hydrogels with longer sampling intervals compared to Fig. 3A.
• Fig. S9. Additional bioactivity data of released siGFP (200 and 350 nM RNA treatment).

Download PDF