LOCUS Exported 13960 bp ds-DNA circular SYN 06-JUN-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSOMA-NLS SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 13960) AUTHORS Patrick Krysan TITLE Direct Submission JOURNAL Exported Jun 6, 2018 from SnapGene 2.8.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13960 /organism="recombinant plasmid" /mol_type="other DNA" misc_feature 22..663 /note="UBQ10 Promoter" CDS 673..3558 /codon_start=1 /product="FRET Biosensor" /note="FRET Sensor CDS" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL EFLTAAGITEGMNELYLEKFSQEQIGENIVCRVICTTGQIPIRDLSADISQVLKEKRSI KKVWTFGRNPACDYHLGNISRLSNKHFQILLGEDGNLLLNDISTNGTWLNGQKVEKNSN QLLSQGDEITVGVGVESDILSLVIFINDKFKQCLEQNKVDRGTSAGGSAGGSAGGSAGG SAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAG GSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSA GGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTS AGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGPGSKKM FWRSASWSASRTASQVPEGDEQSLNIPCAISSGPSRRCPAAPLTPRDHHNSKARACLPP LQPLAISRRSLDEWPGGRMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG KLTLKFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIF FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISGNVYITADKQK NGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFLTAAGITLGMDELSRLPKKKRKVGG" CDS 3529..3549 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /translation="PKKKRKV" terminator 3574..3826 /note="NOS terminator" /note="nopaline synthase terminator and poly(A) signal" misc_feature 4081..4105 /note="RB T-DNA repeat" /note="right border repeat from nopaline C58 T-DNA" rep_origin complement(8466..9054) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(10340..11134) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 11559..11583 /note="LB T-DNA repeat" /note="left border repeat from nopaline C58 T-DNA" CDS complement(11875..12900) /codon_start=1 /product="hygromycin B phosphotransferase" /note="HygR" /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK K" promoter complement(12968..13645) /note="CaMV 35S promoter (enhanced)" /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" ORIGIN 1 aattcgagct cggtacccgg ggatccttgt cgacgagtca gtaataaacg gcgtcaaagt 61 ggttgcagcc ggcacacacg agtcgtgttt atcaactcaa agcacaaata cttttcctca 121 acctaaaaat aaggcaatta gccaaaaaca actttgcgtg taaacaacgc tcaatacacg 181 tgtcatttta ttattagcta ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct 241 cgtcttttct tcttcttctt ctataaaaca atacccaaag agctcttctt cttcacaatt 301 cagatttcaa tttctcaaaa tcttaaaaac tttctctcaa ttctctctac cgtgatcaag 361 gtaaatttct gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa 421 tttcgtatat gttctttggt ttagattctg ttaatcttag atcgaagacg attttctggg 481 tttgatcgtt agatatcatc ttaattctcg attagggttt catagatatc atccgatttg 541 ttcaaataat ttgagttttg tcgaataatt actcttcgat ttgtgatttc tatctagatc 601 tggtgttagt ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta 661 acagaattcg gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 721 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 781 gatgccacct acggcaagct gaccctgaag cttctatgca ccaccggcaa gctgcccgtg 841 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc 901 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 961 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 1021 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1081 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac 1141 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc 1201 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1261 cccgacaacc actacctgag ctaccagtcc gccctgttca aagaccccaa cgagaagcgc 1321 gatcacatgg tcctgctgga gttcctgacc gccgccggga tcactgaggg catgaacgag 1381 ctgtacctcg agaagttttc tcaagaacag atcggcgaaa acattgtgtg cagggtcatt 1441 tgtaccacgg gtcaaattcc catccgagat ttgtcagctg atatttcaca agtgcttaag 1501 gaaaaacgat ccataaagaa agtttggaca tttggtagaa acccagcctg tgactatcat 1561 ttaggaaaca tttcaagact gtcaaataag catttccaaa tactactagg agaagacggt 1621 aaccttttat tgaatgacat ttccactaat gggacctggt taaatgggca aaaagtcgag 1681 aagaacagca atcagttact gtcccaaggt gatgaaataa ccgttggtgt aggcgtggaa 1741 tcagatattt tatctctggt cattttcata aacgacaaat ttaagcagtg cctggagcag 1801 aacaaagttg atcgcggtac cagtgctggt ggtagtgctg gtggtagtgc tggtggtagt 1861 gctggtggta gtgctggtgg ttccggcagt gctggtggta gtgctggtgg tagtaccagt 1921 gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggttcc 1981 ggcagtgctg gtggtagtgc tggtggtagt accagtgctg gtggtagtgc tggtggtagt 2041 gctggtggta gtgctggtgg tagtgctggt ggttccggca gtgctggtgg tagtgctggt 2101 ggtagtacca gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggtagt 2161 gctggtggtt ccggcagtgc tggtggtagt gctggtggta gtaccagtgc tggtggtagt 2221 gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggttccgg cagtgctggt 2281 ggtagtgctg gtggtagtac cagtgctggt ggtagtgctg gtggtagtgc tggtggtagt 2341 gctggtggta gtgctggtgg ttccggcagt gctggtggta gtgctggtgg tagtaccagt 2401 gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggttcc 2461 ggcagtgctg gtggtagtgc tggtggtagt accagtgctg gtggtagtgc tggtggtagt 2521 gctggtggta gtgctggtgg tagtgctggt ggttccggac ctggttctaa gaaaatgttt 2581 tggcggtctg cctcttggtc tgcttcacgg actgcatcac aagttcctga gggtgatgag 2641 caaagcctga acattccgtg tgctattagt tctgggccga gtcgaagatg tccagctgct 2701 cctttgacac ctcgtgatca tcataacagc aaggctagag cttgtttgcc accattgcag 2761 cctcttgcca tttctaggag gagcttagac gagtggcctg gcggccgcat ggtgagcaag 2821 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 2881 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 2941 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 3001 ctgtcctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc 3061 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 3121 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 3181 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 3241 aactacatca gcgggaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc 3301 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag 3361 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 3421 cagtccgcct taagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 3481 ttgaccgccg ccgggatcac tctcggcatg gacgagctgt ctagacttcc taagaagaag 3541 agaaaggttg gaggatagtc gacgaatttc cccgatcgtt caaacatttg gcaataaagt 3601 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat 3661 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt 3721 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca 3781 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggag atctgtcgac 3841 ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 3901 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 3961 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta 4021 gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt 4081 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataacggata 4141 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag 4201 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc 4261 ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg 4321 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata 4381 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct 4441 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc 4501 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc 4561 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg 4621 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc 4681 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg 4741 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc 4801 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc 4861 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc 4921 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca 4981 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag 5041 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa 5101 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg 5161 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc 5221 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg 5281 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct 5341 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga 5401 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc 5461 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga 5521 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt 5581 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt 5641 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga 5701 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt 5761 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt 5821 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta 5881 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc 5941 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc 6001 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc 6061 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg 6121 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag 6181 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta 6241 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat 6301 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga 6361 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg 6421 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca 6481 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc 6541 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa 6601 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg 6661 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac 6721 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt 6781 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc 6841 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc 6901 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg 6961 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag 7021 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt 7081 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc 7141 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct 7201 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc 7261 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc 7321 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc 7381 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg 7441 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc 7501 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa 7561 attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt 7621 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac 7681 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc 7741 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg 7801 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc 7861 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc 7921 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg 7981 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca 8041 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 8101 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg 8161 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga 8221 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 8281 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 8341 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 8401 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 8461 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 8521 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 8581 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 8641 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 8701 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 8761 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 8821 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 8881 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 8941 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 9001 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 9061 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 9121 ttggtcatgc attctaggga aggtgcgaac aagtccctga tatgagatca tgtttgtcat 9181 ctggagccat agaacagggt tcatcatgag tcatcaactt accttcgccg acagtgaatt 9241 cagcagtaag cgccgtcaga ccagaaaaga gattttcttg tcccgcatgg agcagattct 9301 gccatggcaa aacatggtgg aagtcatcga gccgttttac cccaaggctg gtaatggccg 9361 gcgaccttat ccgctggaaa ccatgctacg cattcactgc atgcagcatt ggtacaacct 9421 gagcgatggc gcgatggaag atgctctgta cgaaatcgcc tccatgcgtc tgtttgcccg 9481 gttatccctg gatagcgcct tgccggaccg caccaccatc atgaatttcc gccacctgct 9541 ggagcagcat caactggccc gccaattgtt caagaccatc aatcgctggc tggccgaagc 9601 aggcgtcatg atgactcaag gcaccttggt cgatgccacc atcattgagg cacccagctc 9661 gaccaagaac aaagagcagc aacgcgatcc ggagatgcat cagaccaaga aaggcaatca 9721 gtggcacttt ggcatgaagg cccacattgg tgtcgatgcc aagagtggcc tgacccacag 9781 cctggtcacc accgcggcca acgagcatga cctcaatcag ctgggtaatc tgctgcatgg 9841 agaggagcaa tttgtctcag ccgatgccgg ctaccaaggg gcgccacagc gcgaggagct 9901 ggccgaggtg gatgtggact ggctgatcgc cgagcgcccc ggcaaggtaa gaaccttgaa 9961 acagcatcca cgcaagaaca aaacggccat caacatcgaa tacatgaaag ccagcatccg 10021 ggccagggtg gagcacccat ttcgcatcat caagcgacag ttcggcttcg tgaaagccag 10081 atacaagggg ttgctgaaaa acgataacca actggcgatg ttattcacgc tggccaacct 10141 gtttcgggcg gaccaaatga tacgtcagtg ggagagatct cactaaaaac tggggataac 10201 gccttaaatg gcgaagaaac ggtctaaata ggctgattca aggcatttac gggagaaaaa 10261 atcggctcaa acatgaagaa atgaaatgac tgagtcagcc gagaagaatt tccccgctta 10321 ttcgcacctt ccctaggtac taaaacaatt catccagtaa aatataatat tttattttct 10381 cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga 10441 tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc 10501 ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc 10561 ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat 10621 acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg 10681 ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat 10741 agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga 10801 taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct 10861 cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag 10921 gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc 10981 ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat 11041 ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt 11101 ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca 11161 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct 11221 tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc 11281 gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct 11341 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc 11401 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca 11461 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt 11521 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta 11581 aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactgaatt 11641 aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt taggaattag 11701 aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc 11761 tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa ctactcacac 11821 attattatgg agaaactcga gcttgtcgat cgacagatcc ggtcggcatc tactctattt 11881 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca 11941 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc 12001 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc 12061 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagtcg 12121 tggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca 12181 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat 12241 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga 12301 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag 12361 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg 12421 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat 12481 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc 12541 atccatagcc tccgcgaccg gttgtagaac agcgggcagt tcggtttcag gcaggtcttg 12601 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc taaactcccc 12661 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg 12721 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac 12781 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct 12841 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat 12901 atctcattgc cccccgggat ctgcgaaagc tcgagagaga tagatttgta gagagagact 12961 ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaaggtcttg 13021 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac 13081 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg 13141 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca 13201 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat 13261 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat 13321 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg 13381 ctccaccatg ttatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt 13441 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc 13501 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc 13561 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga 13621 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat 13681 attcttggag tagacgagag tgtcgtgctc caccatgttg gcaagctgct ctagccaata 13741 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 13801 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 13861 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 13921 taacaatttc acacaggaaa cagctatgac catgattacg //