LOCUS Exported 13283 bp ds-DNA circular SYN 06-JUN-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSOMA-T679A-NES SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13283) AUTHORS Patrick Krysan TITLE Direct Submission JOURNAL Exported Jun 6, 2018 from SnapGene 2.8.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13283 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 368..1162 /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 1246..1270 /note="RB T-DNA repeat" /note="right border repeat from nopaline C58 T-DNA" promoter 1916..2261 /note="CaMV 35S promoter" /note="strong constitutive promoter from cauliflower mosaic virus" 5'UTR 2276..2333 /note="AtADH 5'-UTR" /note="translational enhancer from the 5'-UTR of the Arabidopsis thaliana alcohol dehydrogenase gene (Sugio et al., 2008" CDS 2349..5238 /codon_start=1 /product="FRET Biosensor" /note="FRET Sensor CDS" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL EFLTAAGITEGMNELYLEKFSQEQIGENIVCRVICTTGQIPIRDLSADISQVLKEKRSI KKVWTFGRNPACDYHLGNISRLSNKHFQILLGEDGNLLLNDISTNGTWLNGQKVEKNSN QLLSQGDEITVGVGVESDILSLVIFINDKFKQCLEQNKVDRGTSAGGSAGGSAGGSAGG SAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAG GSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSA GGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTS AGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGPGSKKM FWRSASWSASRTASQVPEGDEQSLNIPCAISSGPSRRCPAAPLAPRDHHNSKARACLPP LQPLAISRRSLDEWPGGRMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG KLTLKFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIF FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISGNVYITADKQK NGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFLTAAGITLGMDELSRLQLPPLERLTLD" misc_feature 5197..5238 /note="NES" terminator 5316..5564 /note="HSP terminator" /note="efficient transcription terminator from the Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya et al., 2010)" promoter 5867..6050 /function="NOS promoter" /note="NOS promoter" /note="nopaline synthase promoter" CDS 6071..6865 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" terminator 7139..7391 /note="NOS terminator" /note="nopaline synthase terminator and poly(A) signal" misc_feature 7729..7753 /note="LB T-DNA repeat" /note="left border repeat from nopaline C58 T-DNA" rep_origin 12639..13227 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 gttgccatgt tttacggcag tgagagcaga gatagcgctg atgtccggcg gtgcttttgc 61 cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca 121 ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc atcacccata 181 attgtggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact 241 ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt 301 tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa 361 ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg 421 ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa 481 tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga 541 acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca 601 ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg 661 ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg 721 catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag 781 ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa 841 ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga 901 aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt 961 gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg 1021 gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt 1081 cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat 1141 tttactggat gaattgtttt agtcacatac aaatggacga acggataaac cttttcacgc 1201 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata 1261 tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct gatcctggcg 1321 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga 1381 cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc aggtccccag attagccttt 1441 tcaatttcag aaagaatgct aacccacaga tggttagaga ggcttacgca gcaggtctca 1501 tcaagacgat ctacccgagc aataatctcc aggaaatcaa ataccttccc aagaaggtta 1561 aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc 1621 tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcac aaaccaaggc 1681 aagtaataga gattggagtc tctaaaaagg tagttcccac tgaatcaaag gccatggagt 1741 caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa cagttcatac 1801 agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca 1861 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga 1921 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc 1981 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata 2041 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac 2101 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt 2161 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc 2221 cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggact ctagttacat 2281 cacaatcaca caaaactaac aaaagatcaa aagcaagttc ttcactgttg atacatatgg 2341 aattcggcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 2401 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 2461 ccacctacgg caagctgacc ctgaagcttc tatgcaccac cggcaagctg cccgtgccct 2521 ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc taccccgacc 2581 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 2641 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 2701 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 2761 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc gccgacaagc 2821 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc 2881 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 2941 acaaccacta cctgagctac cagtccgccc tgttcaaaga ccccaacgag aagcgcgatc 3001 acatggtcct gctggagttc ctgaccgccg ccgggatcac tgagggcatg aacgagctgt 3061 acctcgagaa gttttctcaa gaacagatcg gcgaaaacat tgtgtgcagg gtcatttgta 3121 ccacgggtca aattcccatc cgagatttgt cagctgatat ttcacaagtg cttaaggaaa 3181 aacgatccat aaagaaagtt tggacatttg gtagaaaccc agcctgtgac tatcatttag 3241 gaaacatttc aagactgtca aataagcatt tccaaatact actaggagaa gacggtaacc 3301 ttttattgaa tgacatttcc actaatggga cctggttaaa tgggcaaaaa gtcgagaaga 3361 acagcaatca gttactgtcc caaggtgatg aaataaccgt tggtgtaggc gtggaatcag 3421 atattttatc tctggtcatt ttcataaacg acaaatttaa gcagtgcctg gagcagaaca 3481 aagttgatcg cggtaccagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg 3541 gtggtagtgc tggtggttcc ggcagtgctg gtggtagtgc tggtggtagt accagtgctg 3601 gtggtagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggttccggca 3661 gtgctggtgg tagtgctggt ggtagtacca gtgctggtgg tagtgctggt ggtagtgctg 3721 gtggtagtgc tggtggtagt gctggtggtt ccggcagtgc tggtggtagt gctggtggta 3781 gtaccagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg 3841 gtggttccgg cagtgctggt ggtagtgctg gtggtagtac cagtgctggt ggtagtgctg 3901 gtggtagtgc tggtggtagt gctggtggta gtgctggtgg ttccggcagt gctggtggta 3961 gtgctggtgg tagtaccagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg 4021 gtggtagtgc tggtggttcc ggcagtgctg gtggtagtgc tggtggtagt accagtgctg 4081 gtggtagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggttccggca 4141 gtgctggtgg tagtgctggt ggtagtacca gtgctggtgg tagtgctggt ggtagtgctg 4201 gtggtagtgc tggtggtagt gctggtggtt ccggacctgg ttctaagaaa atgttttggc 4261 ggtctgcctc ttggtctgct tcacggactg catcacaagt tcctgagggt gatgagcaaa 4321 gcctgaacat tccgtgtgct attagttctg ggccgagtcg aagatgtcca gctgctcctt 4381 tggcacctcg tgatcatcat aacagcaagg ctagagcttg tttgccacca ttgcagcctc 4441 ttgccatttc taggaggagc ttagacgagt ggcctggcgg ccgcatggtg agcaagggcg 4501 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 4561 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 4621 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgt 4681 cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca 4741 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 4801 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 4861 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 4921 acatcagcgg gaacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact 4981 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga 5041 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt 5101 ccgccttaag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcttga 5161 ccgccgccgg gatcactctc ggcatggacg agctgtctag actgcagctg cctcctctgg 5221 aacgcctgac tctggattaa gtcgacaatt ctgaatcaac aactctcctg gcgcaccatc 5281 gtcggctaca gcctcgggaa ttgctaccga gctcttatga agatgaagat gaaatatttg 5341 gtgtgtcaaa taaaaagcta gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg 5401 tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt aagatctcat tataatgaat 5461 aaacaaatgt ttctataatc cattgtgaat gttttgttgg atctcttcgc atataactac 5521 tgtatgtgct atggtatgga ctatggaata tgattaaaga taaggaattg caattcgtaa 5581 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 5641 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 5701 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca ggatcatgag 5761 cggagaatta agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac 5821 gtttggaact gacagaaccg caacgttgaa ggagccactc agccgcgggt ttctggagtt 5881 taatgagcta agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc 5941 actatcagct agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc 6001 tcggtatcca attagagtct catattcact ctcaatccaa ataatctgca ccggatctgg 6061 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 6121 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 6181 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 6241 gaatgaacta caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 6301 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 6361 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 6421 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 6481 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 6541 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 6601 tatgcccgac ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 6661 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 6721 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 6781 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 6841 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 6901 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 6961 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 7021 gagttcttcg cccacgggat ctctgcggaa caggcggtcg aaggtgccga tatcattacg 7081 acagcaacgg ccgacaagca caacgccacg atcctgagcg acaatatgat cgggccccga 7141 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat 7201 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat 7261 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc 7321 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat 7381 gttactagat cgggaatttg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 7441 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 7501 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggaaccac catcaaacag 7561 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 7621 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccccagta 7681 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca 7741 atatatcctg ccacaagcta gctttctgag ccgccgattt tcctcctcga gttggatgaa 7801 ctcgccgagt tcatcgtcaa ctgaaacaga cacggccgga ttctgtgaga caggttgaac 7861 cgcagctctc ttccattgat aataggtctg aacggaaata cccacgatct taacggcgtc 7921 cttcaaggtt gcgccgccag cgacctgagc ttcgatttga ccgatcttct ccagtttttc 7981 tcggttgctg aggccgcggg ttttcggctt cacggatttg aacgatcccg tgcgggctgt 8041 ttcggctggt gctttctttg ctcttctacc tctaggagca gccggctcaa cttcggcagc 8101 agcagtaccg tccggcggat tctggatctc ttcgtcagcc attaatcgtc ctctgtgtgg 8161 gttattgctt tgtctgccag ctcgatccaa gagtcaacgt ttgtgcctag ggcagtaaat 8221 aggcagtgct ccgcgactac atgcctcggc cggcaaaata ccgccgcatg tagagcaggc 8281 tctccttcac gatcaacgat cggcatgggg ccttcgtgct tgttgagtaa tgttatcgct 8341 cccatcagag cacgcttggt actccgggaa tcggatggtc tgtcgatcat ccaaaaaacg 8401 ctcatgtttt caacctatta ggtctgtggt cagctgacca cagaccatcc tgctccatac 8461 tcgctaattc tagccaaacc gcaacgtccc ctgcccgcta gccttcaaga gcgccattat 8521 catcgggcca agtgaaaact tcccgagttc gctccgccgt gtcagatctc ggagatagcc 8581 cccaggcgaa ttgatgaagt tcgctcgctc caaaatgcac gccatcgctg ctgccgcatt 8641 ctccggtccc attgcctcac acgcgtcttg gtaagccgac gggctgaccc ccagcataga 8701 ccgaaccacc accgcagccg acatgaggtc acgccagcta gcaaccgcac cgctcggccc 8761 ataattgcca atggtcgggc acgctttcag gatcatcccg agggggaacg cttttatcgg 8821 ctcgctcctt gcccggtcta tttcactcgg cttagcgccc tgctcctttt cagagcgagg 8881 ttcaagttca ttaacggatt cgggttttga gttctgtatg tgctgctcgc tctgggcagc 8941 attggtgcta ttattttctg aattgtctct aatttccaac cggttgatta tctcttcctg 9001 gagcatccac atctcttcga gaattgactc tacatcagca agcgtcgggg cgcgtgggat 9061 tctacccaca agttccacat agacttcctc gacagcttgc cagtcgccct ccgctccctc 9121 ttccatagct gccgtaatta gcttccgaac gtcccgtcgg caaatcgtca gactttcttt 9181 ggccatcctg aatgctgctc gatcggccat cacctgctgt gccatcatcg ctagctcttc 9241 ggaccgcgcg agaagcggag acaaatcgaa gccaaacgcg cgctcgatct gaccagcgcc 9301 atccttacga gcgtaacgct ttccgttggc gctatccttc cggacgatca agcctgactc 9361 cacgagcatg gcgatgtgcc tacgcaaagt cgcgccagcc atcccatgcg cccgaagggc 9421 aagctgagca ttcgacggga agacgatcag ctgtgcctcc tgacgcaact ccgtttccgg 9481 gtgaaagctc aatagcgcat caaggacggc aagactgttg gactggattc caagtagttc 9541 catggccgcg gacgcgtccc taaagacctt ccacttgtcc gctgtcttgc cttgtttgat 9601 atcggccagc gccgtctggc gccgcacaag cgcaagcgtc attggccgcc gcccgaatgg 9661 cgtcgttaca cttcctgtct gcatcatctt tcacctttca gcaggcaaag gaaatcagct 9721 caccaaaacg gcgctaaaaa ctcttgacga ggattcgagg aaatgcgatt ctgttcgcgc 9781 tagagagaca gaagggcttc cgcgacggcg acgttgaggg ggctcttttc ttttgcggtt 9841 tactctcccc gtttccgttg gttctcagcg tggtacgctt gatacagcgc tggcacatga 9901 tcgagcacga aggtcgcaaa atcgggcgtc gccttcctgt caatcgtgat ttccagtttg 9961 gccttgctct gcgtcacctg tgcaattctg gtgccgtctg gggtggccat gacctcggga 10021 agtccacgcg caacccgact gggcttcaga ctagcgatca ccgccttgaa tcgttctgcc 10081 gatggcagcg cttgaacttc ctccgacata gcatatttag ccacgtcggc cggtgaagaa 10141 actttctcaa tcagctcggc aagttgttgc caactcggcc gtccaacacc aggagcggca 10201 ccaatagcat cggtcagttc agaggggagg gcgtcaacga gcagaagcat cttggacaaa 10261 ttgctcttgt cgatcgacat cgcggcgatg acaatctctc gagaaaactg cctgttcagg 10321 cgatgtgcga agcgcgcctt ttcgatgaag gtaagatctt cgcgcacatt gttttcctga 10381 ccctgtgcta cgaccacttg ctcgtccgtc agttcgcgaa cgaccgccct gaccggaagt 10441 ccgagttctg aaacggcgcg tagccggcgg tggccgaagg caacctgata tcggcccggc 10501 tggctcggat gcggtcgcac aaggattggg acttgctgtc cttgttcccg gatcgaagta 10561 aggagcccgt caatgtcccc tcgcatacga tcctgcacga aagacggttc tattgacgag 10621 gcatccaact ctatcactgc ctgaccttca gcgagacgcc gctcgatctc ttcggcacgg 10681 ctaagacgat cgttttgctc tcgcagtgcg ttaccaatgt tcgctgtgag cttcgttgcc 10741 ggatcgcgct ccttccttgt tacgccgagg agcggcatgg agcggttctt tgccgtccta 10801 ttgtcggcgg gcgacgtctc aggggcgtca gttgagacgc caaggatgtg cttccggctc 10861 atgtgggcct accccatgct tttttgatca gtgtttcgat ctcgtcgttg acggcgttca 10921 tcgcctccaa ggctcgatca taggtcgagc gcgtgaacag gccacgctcc acttcgaata 10981 gagtctggtt tgtcaggcca gcgtccgaaa ccgcggtggt tttaagcatc ggaaaattga 11041 ggacattttc gccaaaaatc gaccgcagat aacctaccat ttggttctgt ggtccgtcgc 11101 tcggttcgaa acgggttatc agatagcgca tccaattaaa cttgaacttg gcgccagcat 11161 tctcgatttc acgcaaaagg ttcgatgtca ttgccagaaa ctggttcatc gacatcacat 11221 ccagcatctg cggatggacc gtgacaagaa tggacgtcgc cgcagtcaat gcggatagcg 11281 tgagataccc aagctgggga gggcagtcga tgaccacgac gtcatagtta tccgcgatat 11341 cttcaattac ttggctgatg cgaccataaa agagcgtgtc gccctctttg cggttcatca 11401 gcgcgcgtgg cgtatcgtgt tcaaactcca tcagctcaag gttaccagga atcaggtgga 11461 ggtcgggaat gtaagtccct cggacgactc gttcgattgc cacctgctca tcatcatacc 11521 ttatagcgcc gtagagcgtt tcgttcgggc caacgtccgt ctccggttgg ctcccaaaga 11581 gtgcagaaag gctcgcttga ggatcgagat caatggccaa gactcgatat ccgcgcatag 11641 cgaggtactg cgccagatgc gcggcggtgg tggtcttacc cgacccacct ttgaaattca 11701 tcacagagat aacctgaagc tgctcgccgc ctcgacgatg tggcaggtag cgccggttcc 11761 cgcggccgac ctgatccata tacttccgaa tcacatggat atcttcaatt gagaacattc 11821 gcctgccacc tgggctcatg ctaacattca actctggcat ctcagacgcg gtctgccgta 11881 aatatgactc gccaacgccg agcagcttgg acgcctccga tggcccgaat gttcgaatac 11941 ccttctcgga atgcggcggg aaaaccttaa gatgatgtgc ttgaagttgg ctcgagaggg 12001 catcggcatg acgctccatc aaggccgtca accctacaac tacaggcgct gcttttagga 12061 cagacttcgc catctcaaac ccattccttg ccagtggcga tatttttcgc gaaactggaa 12121 aagttccgcc gctggcaatt agcgccgatt ctgctgtttg ggcaagagct tttaggttaa 12181 cagaaggtta acgccctcag gtcgaaaaac tccacccaac tgttatttgt atttatttcc 12241 aatgccttag agagattgcc atttgaatat gttcatgtat tgttttagtg ataatcctac 12301 aatcgtaacc caaaaagagg tcgccctctg cgcgccgtcg tccaatatag gcgaagtcac 12361 ccttgcgact caggcggatt ctaccttgta ggatcgagtc aggcaactat ggatgaacga 12421 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 12481 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 12541 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 12601 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 12661 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 12721 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 12781 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 12841 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 12901 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 12961 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 13021 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 13081 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 13141 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 13201 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 13261 gccttttgct ggccttttgc tca //