The PDF file includes:

• Fig. S1. LPA losses during sample preparation.
• Fig. S2. Live ciliary tracking.
• Fig. S3. SEM cilia coverage of the ventricular surface.
• Fig. S4. LPAR RNA expression in the ventricular region.
• Fig. S5. Dependence of LPA-induced PHH on age, sex, strain, and injection concentration.
• Fig. S6. Data from Fig. 5E analyzed by genotype subgroup and PHH distribution for LPA₁-null pressure measurements.
• Fig. S7. LPAR antagonist efficacy for PHH prevention.
• Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mp4 format). Real-time video of live ciliary beating.
• Movie S2 (.mp4 format). Example of ciliary tracking analysis.