LOCUS pAH144+phi3TI 5732 bp DNA circular 15-FEB-2019 DEFINITION . ACCESSION urn.local...bz-a48udo2 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature 1..18 /label="P3" CDS 13..1095 /label="LacI" misc_feature 49..54 /Recognition_pattern="CAG^CTG" /label="PvuII" /note="Geneious type: restriction site" misc_feature 142..147 /Recognition_pattern="CAG^CTG" /label="PvuII" /note="Geneious type: restriction site" misc_feature 1342..1347 /Recognition_pattern="T^CATGA" /label="BspHI" /note="Geneious type: restriction site" misc_feature 1464..1469 /Recognition_pattern="AT^CGAT" /label="ClaI" /note="Geneious type: restriction site" misc_feature 1471..1488 /label="P1" protein_binding 1506..1527 /label="Lac operator" RBS 1560..1565 misc_feature 1571..1576 /Recognition_pattern="C^CATGG" /label="NcoI" /note="Geneious type: restriction site" misc_feature 1573..1575 /label="start codon" protein_binding 1579..1596 /label="His-Tag" misc_feature 1594..1599 /Recognition_pattern="CAC^GTG" /label="PmlI" /note="Geneious type: restriction site" misc_signal 1615..1629 /label="Enterokinase cut site" misc_feature 1636..1805 /label="T5P25lac-promoter+RBS" /note="Geneious type: promoter prokaryotic" misc_feature 1679..1684 /Recognition_pattern="T^CATGA" /label="BspHI" /note="Geneious type: restriction site" CDS 1806..3137 /label="Phi3TI MT" misc_feature 1812..1817 /Recognition_pattern="A^AGCTT" /label="HindIII" /note="Geneious type: restriction site" misc_feature 1908..1913 /Recognition_pattern="AT^CGAT" /label="ClaI" /note="Geneious type: restriction site" misc_feature 1974..1979 /Recognition_pattern="GAT^ATC" /label="EcoRV" /note="Geneious type: restriction site" misc_feature 2487..2492 /Recognition_pattern="CTGCA^G" /label="PstI" /note="Geneious type: restriction site" misc_feature 2628..2633 /Recognition_pattern="CAG^CTG" /label="PvuII" /note="Geneious type: restriction site" misc_feature 2901..2906 /Recognition_pattern="AT^CGAT" /label="ClaI" /note="Geneious type: restriction site" misc_feature 2909..2914 /Recognition_pattern="C^CATGG" /label="NcoI" /note="Geneious type: restriction site" misc_feature 2979..2984 /Recognition_pattern="CTGCA^G" /label="PstI" /note="Geneious type: restriction site" misc_feature 3039..3044 /Recognition_pattern="CAG^CTG" /label="PvuII" /note="Geneious type: restriction site" misc_feature 3099..3108 /Recognition_pattern="GAANN^NNTTC" /label="XmnI" /note="Geneious type: restriction site" misc_feature 3142..3160 /label="P2" misc_feature 3170..3177 /Recognition_pattern="GG^CGCGCC" /label="AscI" /note="Geneious type: restriction site" misc_feature 3177..3182 /Recognition_pattern="CTGCA^G" /label="PstI" /note="Geneious type: restriction site" misc_feature 3189..3194 /Recognition_pattern="A^AGCTT" /label="HindIII" /note="Geneious type: restriction site" protein_binding 3216..3260 /label="S-Tag" misc_feature 3275..3282 /Recognition_pattern="TTAAT^TAA" /label="PacI" /note="Geneious type: restriction site" misc_feature 3294..3311 /label="P4" misc_feature 3887..3892 /Recognition_pattern="C^CATGG" /label="NcoI" /note="Geneious type: restriction site" misc_feature 4011..4016 /Recognition_pattern="A^AGCTT" /label="HindIII" /note="Geneious type: restriction site" misc_feature 4355..5365 /label="CDS 1" misc_feature 5143..5148 /Recognition_pattern="T^CATGA" /label="BspHI" /note="Geneious type: restriction site" misc_feature 5433..5438 /Recognition_pattern="AT^CGAT" /label="ClaI" /note="Geneious type: restriction site" misc_feature 5716..5721 /Recognition_pattern="A^AGCTT" /label="HindIII" /note="Geneious type: restriction site" ORIGIN 1 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 61 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct 121 tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg 181 cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa 241 cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatgtccgc 301 accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt 361 ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa 421 accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt 481 gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc 541 taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc 601 gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa 661 cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata 721 gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc 781 ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg 841 agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac 901 gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt 961 cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg 1021 gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa 1081 cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat 1141 accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg 1201 actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa 1261 ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca 1321 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg 1381 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga 1441 tgcgtccggc gtagaggatc gagatcgatc tcgatcccgc gaaattaata cgactcacta 1501 taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga 1561 aggagatata ccatggcaca tcaccaccac catcacgtgg gtaccggttc gaatgatgac 1621 gacgacaaga gtcccaattg tgagcggata acaattacga gcttcatgca cagtgaaatc 1681 atgaaaaatt tatttgcttt gtgagcggat aacaattata atatgtggaa ttgtgagcgc 1741 tcacaattcc acaacggttt ccctctagaa ataattttgt ttaactttta taggaggtgt 1801 tccatatgtc aaagcttcgc gttatgtcct tattttccgg tataggcgcc tttgaggcgg 1861 cccttagaaa tataggtgtt gactatgaac ttataggttt tagcgagatc gataaatatg 1921 ctattaaatc atattgcgcc atccataacg tatcagagac tttgaatgtg ggtgatatca 1981 gtaaagcgaa aaaagataat ataccatatt ttgacctgtt aacttctggt tttccctgtc 2041 ctacctttag cgttgcaggc ggcagagatg gtatggaata caaatgttcg aattgtagcc 2101 acgaacattt aattacgtac gaagactata aaaagggtgt taaatgccca aaatgtgagg 2161 cggtcagcaa ggcaaaagat gagcgcggta cgttgttttt tgaaacggcg ttactggccg 2221 aggaaaaaaa gccgaaattt gtgatcctgg aaaatgtaaa aggtttgatt aactccggaa 2281 atggccaggt cttacgtatc ataagtgaaa caatgaataa tattggttac cgcatagatc 2341 tggagctgtt aaacagtaag ttcttcaatg tgccacagaa tcgcgaacgt gtgtatatca 2401 ttggtattcg tgaagacttg gtggaaaatg aacaatgggt ggtgggtcag aagcggaatg 2461 atgtcttaag caaggggaag aagcgtctgc aggaaattaa tatcaagtcg tttaacttta 2521 aatggccgct tcaagacaca gtcactaagc gtctgcgcga gattttagaa gattttgtgg 2581 acgagaaata ctatcttaac gaggaaaaga caaaaaaact ggtggagcag ctgggcacgg 2641 ccccgcttca gaagcaggaa gtccgcgaac cgttgatggt aggccatgtg gatcttaaag 2701 gtcacgatgc tataaaacgc gtgtacagcc cggaagggct gtctccaact ctgacgacga 2761 tgggtggagg acatcgcgaa cccaaaatag ctgaaaaaca aaaagaagta cgggcagtcc 2821 tgacccctga acgcgaagaa aaacggcaga atggaagacg tttcaaagag aatggcgagc 2881 cggcgtttac tgttaataca atcgatcgcc atggcgttgc cataggcgaa tacccaaaat 2941 ataaaattcg taaattgagc cctttagaat gctggcggct gcaggcattc gatgacgagg 3001 atttcgaaaa agcctttgcg gcgggaatca gtaatagcca gctgtacaag caggcgggaa 3061 acagtatcac ggtatcagtc ttggagtcga tatttcagga actgattcac acatatgtta 3121 acaaagaaag cgaataatat gggtatccca attgggagct cgtgtacacg gcgcgcctgc 3181 aggtcgacaa gcttgcggcc gcactcgagt ctggtaaaga aaccgctgct gcgaaatttg 3241 aacgccagca catggactcg tctactagcg cagcttaatt aacctaggct gctgccaccg 3301 ctgagcaata aaattctcat gtttgacagc ttatcactga tcagtgaatt aatggcgatg 3361 acgcatcctc acgataatat ccgggtaggc gcaatcactt tcgtctctac tccgttacaa 3421 agcgaggctg ggtatttccc ggcctttctg ttatccgaaa tccactgaaa gcacagcggc 3481 tggctgagga gataaataat aaacgagggg ctgtatgcac aaagcatctt ctgttgagtt 3541 aagaacgagt atcgagatgg cacatagcct tgctcaaatt ggaatcaggt ttgtgccaat 3601 accagtagaa acagacgaag aagctagcta atgctctgtc tcaggtcact aatactatct 3661 aagtagttga ttcatagtga ctggatatgt tgcgttttgt cgcattatgt agtctatcat 3721 ttaaccacag attagtgtaa tgcgatgatt tttaagtgat taatgttatt ttgtcatcct 3781 ttaggtgaat aagttgtata tttaaaatct ctttaattat cagtaaatta atgtaagtag 3841 gtcattatta gtcaaaataa aatcatttgt cgatttcaat tttgtcccat ggctaattcc 3901 catgtcagcc gttaagtgtt cctgtgtcac tcaaaattgc tttgagaggc tctaagggct 3961 tctcagtgcg ttacatccct ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa 4021 agccttatat attctttttt ttcttataaa acttaaaacc ttagaggcta tttaagttgc 4081 tgatttatat taattttatt gttcaaacat gagagcttag tacgtgaaac atgagagctt 4141 agtacgttag ccatgagagc ttagtacgtt agccatgagg gtttagttcg ttaaacatga 4201 gagcttagta cgttaaacat gagagcttag tacgtgaaac atgagagctt agtacgtact 4261 atcaacaggt tgaactgctg atcttcagat cctctacgcc ggacgcatcg tggccggatc 4321 ttgcggccgc tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct 4381 cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt 4441 cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca 4501 ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc 4561 tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg 4621 gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg 4681 gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg 4741 tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt 4801 cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga 4861 tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg 4921 aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta 4981 cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg 5041 agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta 5101 cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactttg 5161 ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac 5221 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac 5281 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg 5341 ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga accgaacagg 5401 cttatgtcca ctgggttcgt gccttcatcc gtatcgatgg cccccgatgg tagtgtgggg 5461 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa 5521 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa 5581 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg 5641 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt 5701 tgcgtggcca gtgccaagct tgcatgcctg ca //