LOCUS pAH55+Cthe_0519 6908 bp DNA circular 15-FEB-2019 DEFINITION . ACCESSION urn.local...c4-a48udoc KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers RBS 90..95 misc_feature 101..106 /Recognition_pattern="C^CATGG" /label="NcoI" /note="Geneious type: restriction site" protein_binding 109..126 /label="His-Tag" misc_feature 124..129 /Recognition_pattern="CAC^GTG" /label="PmlI" /note="Geneious type: restriction site" misc_signal 145..159 /label="Enterokinase cut site" misc_feature 166..335 /label="T5P25lac-promoter+RBS" /note="Geneious type: promoter prokaryotic" misc_feature 209..214 /Recognition_pattern="T^CATGA" /label="BspHI" /note="Geneious type: restriction site" CDS 336..2168 /label="Cthe0519-T2MT" misc_feature 648..653 /Recognition_pattern="CTGCA^G" /label="PstI" /note="Geneious type: restriction site" misc_feature 737..742 /Recognition_pattern="T^CATGA" /label="BspHI" /note="Geneious type: restriction site" misc_feature 1050..1059 /Recognition_pattern="GAANN^NNTTC" /label="XmnI" /note="Geneious type: restriction site" misc_feature 1147..1152 /Recognition_pattern="CAC^GTG" /label="PmlI" /note="Geneious type: restriction site" misc_feature 1370..1375 /Recognition_pattern="CAC^GTG" /label="PmlI" /note="Geneious type: restriction site" misc_feature 1605..1610 /Recognition_pattern="CAG^CTG" /label="PvuII" /note="Geneious type: restriction site" misc_feature 2173..2191 /label="Reverse" misc_feature 2896..2901 /Recognition_pattern="C^CATGG" /label="NcoI" /note="Geneious type: restriction site" misc_feature 3020..3025 /Recognition_pattern="A^AGCTT" /label="HindIII" /note="Geneious type: restriction site" misc_feature 3387..4181 /modified_by="lrf" /label="Kan" misc_feature 3616..3621 /Recognition_pattern="C^CATGG" /label="NcoI" /note="Geneious type: restriction site" misc_feature 3944..3949 /Recognition_pattern="CAG^CTG" /label="PvuII" /note="Geneious type: restriction site" misc_feature 4248..4253 /Recognition_pattern="T^CATGA" /label="BspHI" /note="Geneious type: restriction site" misc_feature 4333..4338 /Recognition_pattern="AT^CGAT" /label="ClaI" /note="Geneious type: restriction site" misc_feature 4616..4621 /Recognition_pattern="A^AGCTT" /label="HindIII" /note="Geneious type: restriction site" misc_feature 4628..4633 /Recognition_pattern="CTGCA^G" /label="PstI" /note="Geneious type: restriction site" misc_feature 5070..6029 /label="laqIQ" misc_feature 5745..5750 /Recognition_pattern="GAT^ATC" /label="EcoRV" /note="Geneious type: restriction site" misc_feature 5895..5900 /Recognition_pattern="CAG^CTG" /label="PvuII" /note="Geneious type: restriction site" misc_feature 5988..5993 /Recognition_pattern="CAG^CTG" /label="PvuII" /note="Geneious type: restriction site" misc_feature 6391..6396 /Recognition_pattern="AT^CGAT" /label="ClaI" /note="Geneious type: restriction site" misc_feature 6663..6668 /Recognition_pattern="A^AGCTT" /label="HindIII" /note="Geneious type: restriction site" ORIGIN 1 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc 61 ctctagaaat aattttgttt aactttaaga aggagatata ccatggcaca tcaccaccac 121 catcacgtgg gtaccggttc gaatgatgac gacgacaaga gtcccaattg tgagcggata 181 acaattacga gcttcatgca cagtgaaatc atgaaaaatt tatttgcttt gtgagcggat 241 aacaattata atatgtggaa ttgtgagcgc tcacaattcc acaacggttt ccctctagaa 301 ataattttgt ttaactttta taggaggtgt tccatatgat cgagactacc ctgactggca 361 agacccctga tattggagaa gaaaacatta aaaagttaat gacgatgttt ccggaagtcg 421 tgactgaagg caaggtggat ttcgaaaaac ttaagcagct tctgggtgaa tatgtagatg 481 attccaacga acggtacaac ttcacatgga atggcaaagg tcgcgcatta cgcttaagcc 541 agacaccgtc cttagggacg ctgcggccgt gtaaggagga atcgaaaaac tgggatacga 601 ctcagaatct gtatattgag ggagacaatc tggaggttct taagttgctg cagaagagtt 661 attatggtaa aatcaaaatg atttatatag atccgccata caatacaggg aaagactttg 721 tctaccgtga tgattttcat gactcattgg agaactataa acggattacg ggccaggtgg 781 acggaaacgg caaagccata agcacaaaca cagagacgtc aggccgttat cacacggatt 841 ggctgaacat gatgtatccg agattacggc tggcacgcaa tctgctgtcg gacgacggcg 901 tgatatttat ttccattgat gataacgaag ttgataatct taaaaaaatc tgcaacgaaa 961 tctttggcga ggataatttt atcgcaaatt gcgtccgcaa acgcagagat agccaggcga 1021 atctgagcca aaacatcagt cctattcacg aatatgttct tatctacgcg aaaagatttg 1081 ggaacatact taataaagtt acaccgagtt tggatatggg ctcctataaa aatccagaca 1141 atgacccacg tgggccgtat acaacgatgc cgtgtacaaa cgtgggtggg gccgtttata 1201 gcattgtgac ccccactggc aaaacgatta ctgacgaatg gcgttttaaa aaagagacgt 1261 ttgaaaagct gctgcttgac aaccgcatag tatttccacg taacggagaa ggcaaaccca 1321 gatacaaaat attcttaagc gaaaagatgg cggaaggagt tttagcgaac acgtggctgg 1381 ataagattgc aagtaatcag gaggggacgc gcgaaattaa agaactgttt ggcggtttgc 1441 tgtttaacaa tccgaaacca actggcctgt tgaagttcct gttagaactt ggtagctcta 1501 aagactcaat tattttagac tttttcagtg gtagtgcaac cacagcgcat gcggtaatgc 1561 agcttaatgc cgaggatgga ggcaatagaa gattcataat ggtccagctg cccgaaccta 1621 ccgacgaaaa ttcagaagcc tacaaagcag gctatatgaa tattagcgaa atcggaaagg 1681 aacgtatacg tcgggcaggc gaaaagatca aagaagaata taaagataaa gggaatatcg 1741 agaatttaga tattggattt aaagttttta aactggacac cagcaacata cgtaaatggc 1801 aacccgatta cgataattta gagcaaagtc ttatggatta cgtggacaat tttgtcgaag 1861 gtagaaccga actggatgtg gtttatgaaa taatgttaaa atatggcctt gatcttacat 1921 atccggtgga cgagttcacc attgctggta agaaagtata tagtattgga tacggtatgt 1981 taatgatctg tctggacaac gaaataacta ccgaagttgc aaagggtatt cttactaaga 2041 ttaaagaatt atccccagag agttccagag tggtttttaa agataatggc tttaaaaccg 2101 attctaataa gactaatata aaagagatat taaagagtgg tggtatagag gagtttataa 2161 caatttaata tgggtatccc aattgggagc taattctcat gtttgacagc ttatcactga 2221 tcagtgaatt aatggcgatg acgcatcctc acgataatat ccgggtaggc gcaatcactt 2281 tcgtctctac tccgttacaa agcgaggctg ggtatttccc ggcctttctg ttatccgaaa 2341 tccactgaaa gcacagcggc tggctgagga gataaataat aaacgagggg ctgtatgcac 2401 aaagcatctt ctgttgagtt aagaacgagt atcgagatgg cacatagcct tgctcaaatt 2461 ggaatcaggt ttgtgccaat accagtagaa acagacgaag aagctagctt tgcactggat 2521 tgcgaggctt tgtgcttctc tggagtgcga caggtttgat gacaaaaaat tagcgcaaga 2581 agacaaaaat caccttgcgc taatgctctg ttacaggtca ctaataccat ctaagtagtt 2641 gattcatagt gactgcatat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc 2701 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttttta 2761 tactaagttg gcattataaa aaagcattgc ttatcaattt gttgcaacga acaggtcact 2821 atcagtcaaa ataaaatcat tatttgattt caattttgtc ccactccctg cctctgtcat 2881 cacgatactg tgatgccatg gctaattccc atgtcagccg ttaagtgttc ctgtgtcact 2941 caaaattgct ttgagaggct ctaagggctt ctcagtgcgt tacatccctg gcttgttgtc 3001 cacaaccgtt aaaccttaaa agctttaaaa gccttatata ttcttttttt tcttataaaa 3061 cttaaaacct tagaggctat ttaagttgct gatttatatt aattttattg ttcaaacatg 3121 agagcttagt acgtgaaaca tgagagctta gtacgttagc catgagagct tagtacgtta 3181 gccatgaggg tttagttcgt taaacatgag agcttagtac gttaaacatg agagcttagt 3241 acgtgaaaca tgagagctta gtacgtacta tcaacaggtt gaactgctga tcttcagatc 3301 ctctacgccg gacgcatcgt ggccggatct tgcggccgca aaaattaaaa atgaagtttt 3361 gacggtatcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg 3421 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg 3481 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc 3541 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc 3601 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat ccgcgccttg 3661 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga 3721 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg 3781 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg 3841 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc 3901 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg 3961 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt ggagttcatt cagggcaccg 4021 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg 4081 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa 4141 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct 4201 gtctcttgat ccactagatt attgaagcat ttatcagggt tattgtctca tgagcggata 4261 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 4321 agtgccacct gcatcgatgg cccccgatgg tagtgtgggg tctccccatg cgagagtagg 4381 gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 4441 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga 4501 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc 4561 atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtggcca gtgccaagct 4621 tgcatgcctg cagcatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 4681 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 4741 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 4801 aaacgcgcga ggcagccgga tcaattctca tgtttgacag cttatcatcg agcttactcc 4861 ccatccccga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg 4921 aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt 4981 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg 5041 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg 5101 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg 5161 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg 5221 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc 5281 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg 5341 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg 5401 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg 5461 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg 5521 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc 5581 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa 5641 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg 5701 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg 5761 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg 5821 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg 5881 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc 5941 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 6001 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 6061 cattaggcac cccaggcttt acactttatg cttccgggtt ttctttgcct cacgatcgcc 6121 cccaaaacac ataaccaatt gtatttattg aaaaataaat agatacaact cactaaacat 6181 agcaattcag atccggtcca gtaatgacct cagaactcca tctggatttg ttcagaacgc 6241 tcggttgccg ccgggcgttt tttattggtg agaatcgcag caacttgtcg cgccaatcga 6301 gccatgtcgt cgtcaacgac cccccattca agaacagcaa gcagcattga gaactttgga 6361 atccagtccc tcttccacct gctgatccgg atcgatcccg ttatcgactg cacggtgcac 6421 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat 6481 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg 6541 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc 6601 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacaga attgatccgg 6661 ccaagcttgt aagaaggaga tatacatatg gcgagacgta ttctggtcgt agaagatgaa 6721 gctccaattc gcgaaatggt ctgcttcgtg ctcgaacaaa atggctttca gccggtcgaa 6781 gcggaagatt atgacagtgc tgtgaatcaa ctgaatgaac cctggccgga tttaattctc 6841 ctcgactgga tgttacctgg cggctccggt atccagttca tcaaacacct caagcgcgag 6901 tcgatgac //